基礎生物学研究所
| Organizers | Robert E. Braun (The Jackson Laboratory) Mark Van Doren (The Jackson Laboratory) Satoru Kobayashi (NIBB) Shosei Yoshida (NIBB) |
|---|---|
| Venue | Okazaki Conference Center, Okazaki, Japan |
| Date | Jul. 17-21, 2012 |
| Link | オフシャルサイト （http://www.nibb.ac.jp/conf58/） |
| Poster |
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「生殖細胞系列」
第58回および60回のNIBBカンファレンス“Germline –Specification, Sex, and Stem Cells- を、2012年7月17日（火）～21日（土）に岡崎コンファレンスセンターにて開催した。当初、第58回”Gamete Stem Cells”を2011年7月に開催する予定であったが、東日本大震災の影響により止む無く延期となった。2012年7月には、第60回カンファレンス”Germline Development”を開催予定であったため、異例の「第58回/第60回合同カンファレンス」として開催するに至った。
本シンポジウムは、生殖細胞系列研究の中心的な３つの課題、生殖細胞の成立、生殖細胞の性、配偶子の幹細胞に焦点を絞り、ほ乳類（霊長類、マウス）、鳥類、魚類、ホヤ、ヒドラ、線虫、プラナリア、ショウジョウバエなど、広く動物種を越えてその普遍性と特殊性を議論した。国内外より130名を越える参加者を得ることとなった。本研究領域を牽引する４名の研究者による特別講演をはじめ、最新のデータ（多くは未発表）に基づく力のこもった発表と白熱した議論が終始展開されたのが印象的であった。
特別講演に引き続き、生殖細胞の成立および性に関する最先端の講演が続いた。生殖細胞の雌化あるいは雄化を細胞自律的に引き起こす遺伝子の同定、体細胞による生殖細胞の性の制御機構、生殖細胞における遺伝子の活性化や遺伝子発現抑制に関わる分子機構等がトピックスであり、それらに関わる遺伝子や分子機構の進化的保存性に関する論議が展開された。また、鳥、プラナリア、ホヤ、ヒドラ等を用いたユニークな生殖細胞研究も紹介され、限られたモデル生物だけではない生殖細胞研究の裾野の広がりを感じることができた。配偶子幹細胞については、マウス、霊長類、魚類、ショウジョウバエなど多彩な生物を対象とする研究者が一堂に会して議論した歴史的機会となった。互いに異なる組織形態を示すショウジョウバエやマウス、その他多くの動物の配偶子幹細胞システムの共通点や、幹細胞の動態についてのコンピュータシュミレーションや数理統計解析を基盤とした議論が展開された。また、医学、畜産学、水産学的観点から生殖細胞を操作する多くの革新的技術も、本シンポジウムの重要なテーマの一つであり、多彩な議論が展開された。
タイトなスケジュールではあったが、会議の合間や懇親会や食事会で参加者同志が密接に時を過ごし、多くの関係が築かれたと思う。今後の本研究領域の発展のための種となれば幸甚である。
最後に、参本シンポジウムに参加されたすべての方々と、運営にあたった国際連携室およびオーガナイザー研究室の方々、共催の文部科学省科学研究費補助金「配偶子幹細胞制御機構」、サポートをいただいた井上科学振興財団、大幸財団に感謝する。（吉田 松生）
| July 17th (Tue) at the Okazaki New Grand Hotel | |
| 19：00 - | A reception for invited speakers |
| July 18th (Wed) at the Okazaki Conference Center (OCC) | |
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The 60th NIBB Conference
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| Opening Remarks | |
| 09:00 - 09:10 | Kiyotaka Okada (Director-General of the National Institute for Basic Biology, Japan) |
| 09:10 - 09:20 | Satoru Kobayashi & Shosei Yoshida (National Institute for Basic Biology, Japan) |
| Special Lectures | |
| Mark Van Doren, Satoru Kobayashi | |
| 09:20 - 10:20 |
“Silencing the Germline” Ruth Lehmann (New York University, USA) |
| 10:20 - 10:30 | Coffee Break |
| 10:30 - 11:30 |
“Development in vivo of Fertilization-Competent Oocyte-like Cells in the Absence of Meiotic Initiation or Prophase” David C Page (Whitehead Institute for Biomedical Research, USA) |
| 11:30 - 13:00 | Lunch Break |
| Session I | Regulatory Mechanism of Germline Specification |
| Akira Nakamura, Geraldine Seydoux | |
| 13:00 - 13:40 |
“RNA Silencing in Germline Stem Cells” Geraldine Seydoux (Johns Hopkins University, USA) |
| 13:40 - 14:20 |
“Germline Transcriptional Repression in Drosophila and Ciona Embryos” Akira Nakamura (RIKEN Center for Developmental Biology, Japan) |
| 14:20 - 14:40 | Coffee Break |
| 14:40 - 15:20 |
“PEM, a Localized Maternal Factor Unique to Ascidians, Represses Germline Gene Expression through RNAP II Regulation and Binding to p-TEFb” Gaku Kumano (Osaka University, Japan) |
| 15:20 - 16:00 |
“Mechanism and Reconstitution In Vitro of Germ Cell Specification in Mice” Mitinori Saitou (Kyoto University, Japan) |
| 16:00 - 16:30 | Coffee Break |
| Short Talks I | |
| Yasuhisa Matsui, Mitinori Saitou | |
| 16:30 - 16:45 |
“Primordial Germ Cells Transmigrate from Blood Stream to Gonad in Avian Embryos: Novel Behavior Revealed by Live-imaging Analyses” Daisuke Saito (Nara Institute of Science and Technology, Japan) |
| 16:45 - 17:00 |
“PRDM14 Promotes Active DNA Demethylation through the Base Excision Repair Pathway in Embryonic Stem Cells” Yoshiyuki Seki (Kwansei Gakuin University, Japan) |
| 17:00 - 17:15 |
“NANOS2-mediated RNA Regulation in the Differentiation of Mouse Male Gonocytes” Atsushi Suzuki (Yokohama National University, Japan) |
| 17:15 - 17:35 | Coffee Break |
| 17:35 - 17:50 |
“Independent Evolution of Sex Chromosomes through Recurrent Use of Sox3 in Medaka Fish and Mammals” Yusuke Takehana (National Institute for Basic Biology, Japan) |
| 17:50 - 18:05 |
“Effect of Fetal Leydig Cell-Specific Ad4BP/SF-1 Gene Disruption on Masculinization and Spermatogenesis” Yuichi Shima (Kyushu University, Japan) |
| 18:05 - 18:20 |
“Y Chromosomal Azoospermic Factor RBMY and Estrogen Signaling Coordinately Regulate Male-specific Alternative Splicing” Matomo Sakari (University of Tokushima, Japan) |
| 18:20 - 18:35 |
“The Germ Plasm-dependent and -independent Mechanisms for the Germ Cell Specification in the Ascidian, C. intestinalis” Maki Shirae-Kurabayashi (Hiroshima University, Japan) |
| 19:00 - 21:00 | Mixer |
| July 19th (Thu) at OCC | |
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The 60th NIBB Conference
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| Session II | Regulatory Mechanism of Germline Sex |
| Mark Van Doren, Peter Koopman | |
| 09:00 - 09:40 |
“Sexual Dimorphism in the Gonad Stem Cell Niche” Mark Van Doren (Johns Hopkins University, USA) |
| 09:40 - 10:20 |
“Mechanism Regulating Germline Sexual Identity in Drosophila Embryos” Satoru Kobayashi (National Institute for Basic Biology, Japan) |
| 10:20 - 10:40 | Coffee Break |
| 10:40 - 11:20 |
“Molecules That Maintain and Regulate Germ Cells during Sexual Differentiation of Medaka Gonads” Minoru Tanaka (National Institute for Basic Biology, Japan) |
| 11:20 - 12:00 |
“Factors Influencing the Sex-specific Differentiation of Fetal Germ Cells” Peter Koopman (The University of Queensland, Australia) |
| 12:00 - 12:40 |
“Genetic Pathways Leading to Sexual Fate Decision in Mouse Germ Cells” Yumiko Saga (National Institute of Genetics, Japan) |
| 12:40 - 13:40 | Lunch Break |
| Poster Session I | |
| 13:40 - 15:30 | Poster Session for odd-numbered posters Poster Session List (pdf 73KB) |
| Session III | Pluripotent Stem Cells and Germline |
| Kazuya Kobayashi, Phillip A Newmark | |
| 15:30 - 16:10 |
“Max is a Repressor of Germ-cell-related Gene Expression in Mouse Embryonic Stem Cells” Yasuhisa Matsui (Tohoku University, Japan) |
| 16:10 - 16:50 |
“Sex-inducing Effects by ‘Feeding’: Switching from Asexual to Sexual Reproduction in the Planarian Dugesia ryukyuensis” Kazuya Kobayashi (Hirosaki University, Japan) |
| 16:50 - 17:30 |
“Germ Cell Development and Regeneration in Planarians:Implications for Understanding Parasitic Flatworms” Phillip A Newmark (University of Illinois, USA) |
| 17:30 - 17:50 | Coffee Break |
| Short Talks II | |
| Yumiko Saga, Minoru Tanaka | |
| 17:50 - 18:05 |
“In Vitro Propagation of Presumptive Germ Cells with Primordial Germ Cell Characteristics Derived from Mouse Induced Pluripotent Stem Cells” Masanori Imamura (Keio University, Japan) |
| 18:05 - 18:20 |
“Acquisition of Pluripotency from Primordial Germ Cells” Go Nagamatsu (Keio University, Japan) |
| 18:20 - 18:35 |
“Nutrition-dependent Control of Ovarian Development by the Fat Body in Drosophila” Hiroko Sano (Kurume University, Japan) |
| 18:35 - 18:50 |
“A Transient Niche for the Germline Stem Cell Precursors in Drosophila Ovary” Miho Asaoka (National Institute of Genetics, Japan) |
| 18:50 - 19:05 |
“t-SNARE Syntaxin2 is Required for Transportation of Glycolipids and Stable Formation of Intercellular Bridges during Spermatogenesis” Yasuhiro Fujiwara (Okayama University, Japan) |
| 19:05 - 19:20 |
“Analysis of Epigenetic Changes Associating with Spermatogonial Stem Cell Differentiation” Kazuyuki Ohbo (Yokohama City University, Japan) |
| July 20th (Fri) at OCC | |
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The 58th NIBB Conference
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| Special Lectures | |
| Robert E Braun, Shosei Yoshida | |
| 09:00 - 10:00 |
“The Germline: Evolution’s Trustee” Allan C Spradling (Carnegie Institution for Science, USA) |
| 10:00 - 10:10 | Coffee Break |
| 10:10 - 11:10 |
“Spermatogonial Stem Cells: Computer Simulation of Their Behaviour in the Stem Cell Niche” Dirk G de Rooij (University of Amsterdam, Netherlands) |
| Short Talks III | |
| Yuzo Niki, Kyle E Orwig | |
| 11:10 - 11:25 |
“Propagation of Nile-tilapia Spermatogonial Stem Cells In Vitro” Samyra Lacerda (Universidade Federal de Minas Gerais, Brazil) |
| 11:25 - 11:40 |
“Establishment of Long-term Culture Conditions for Zebrafish Spermatogonial Stem Cells” Noriyoshi Sakai (National Institute of Genetics, Japan) |
| 11:40 - 11:55 |
“LIF (Leukemia Inhibitory Factor) Promotes Spermatogonial Proliferation in Medaka (Oryzias latipes)” Ryuichi Satoh (Hokkaido University, Japan) |
| 11:55 - 12:10 |
“Low Temperature-induced Circulating T3 Activates Metamorphic Genes and Accelerates Seasonal Testicular Regression” Yusuke Atsumi (Nagoya University, Japan) |
| 12:10 - 13:10 | Lunch Break |
| Poster Session II | |
| 13:10 - 14:50 | Poster Session for even-numbered posters Poster Session List (pdf 73KB) |
| Session IV | Germline Stem Cell Functionality In Vivo |
| Benjamin D Simons, Erika Matunis | |
| 14:50 - 15:30 |
“Stem Cell Renewal in the Drosophila Testis Niche” Erika Matunis (Johns Hopkins University, USA) |
| 15:30 - 16:10 |
“Cellular and Molecular Analyses of a Stable Drosophila Germline Stem Cell Niche Reconstructed In Vitro” Yuzo Niki (Ibaraki University, Japan) |
| 16:10 - 16:30 | Coffee Break |
| 16:30 - 17:10 |
“How are the Self-renewal and Differentiation of Mouse Spermatogenic Stem Cells Balanced?” Shosei Yoshida (National Institute for Basic Biology, Japan) |
| 17:10 - 17:50 |
“The Population Asymmetric Self-renewal of Germ Line Stem Cells” Benjamin D Simons (University of Cambridge, UK) |
| 18:30 - 20:30 | Mixer |
| July 21st (Sat) at OCC | |
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The 58th NIBB Conference
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| Session V | Regulatory Mechanism of Germline Stem Cells |
| Robert E Braun, Takashi Shinohara | |
| 09:00 - 09:40 |
“Functional Compartmentalization of Murine Germ Cells and Translocation of Syncytial Chains across Sertoli Cell Tight Junctions” Robert E Braun (The Jackson Laboratory, USA) |
| 09:40 - 10:20 |
“In Vitro Transformation of Male Germline Stem Cells” Takashi Shinohara (Kyoto University, Japan) |
| 10:20 - 11:00 |
“Translating Spermatogonial Stem Cell Transplantation Toward the Clinic” Kyle E Orwig (University of Pittsburgh, USA) |
| 11:00 - 12:00 | Lunch Break |
| Session VI | Manipulation of Germ Cells |
| Takehiko Ogawa, Goro Yoshizaki | |
| 12:00 - 12:40 |
“Avian Primordial Germ Cell: Its Culture and Manipulation for Transgenesis” Jae Yong Han (Seoul National University, Korea) |
| 12:40 - 13:20 |
“In Vitro Spermatogenesis Using an Organ Culture Method” Takehiko Ogawa (Yokohama City University, Japan) |
| 13:20 - 14:00 |
“Germ Cell Transplantation in Fish: Can Mackerel Produce Tuna?” Goro Yoshizaki (Tokyo University of Marine Science and Technology, Japan) |
| Closing Remarks | |
| 14:00 - 14:10 | Shosei Yoshida & Satoru Kobayashi (National Institute for Basic Biology, Japan) |
