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大学共同利用機関法人 自然科学研究機構

基礎生物学研究所

国際連携

プラクティカルコース - 開催リスト

4th course "The NIBB Laboratory Course and Workshops on Physcomitrella patens 2009"

Organizers Andrew Cuming (Univ. Leeds)
Yuji Hiwatashi (NIBB)
Yasuko Kamisugi (Univ. Leeds)
Minoru Kubo (ERATO, JST)
Tetsuya Kurata (ERATO, JST)
Takashi Murata (NIBB)
Tomoaki Nishiyama (Kanazawa Univ.)
Keiko Sakakibara (ERATO, JST)
Yoshikatsu
Venue National Institute for Basic Biology
Date Jun. 29-Jul. 3, 2009
Link Website (http://www.nibb.ac.jp/course/past/4th/)
Poster 4th course
practical4-group_photo.jpg
 コケ植物のヒメツリガネゴケは、遺伝子ターゲティングが容易で種々の実験に適しており、基生研を日本の代表とした国際コンソーシアムによってゲノムも解読されました。世界中で新しいモデル植物として用いられるようになりました。基礎生物学研究所では世界に先駆けて多くの実験技術を開発してきた経緯もあり、広く実験技術の普及を行っています。今回は第二回目として、英国リーズ大学よりAndrew Cuming 博士、Yasuko Kamisugi 博士、科学技術振興機構ERATO 分化全能性進化プロジェクトより5名の講師を招聘し、基礎生物学研究所生物進化研究部門が中心となって、5日間のコースを開催しました。ヒメツリガネゴケを用いた基礎から最先端までの実験技術が集約された約300ページの英文マニュアルを用いて、連日、朝9時から夜9時すぎまで、講義と実習がおこわれわれました。また、未発表の最新実験技術について、次世代シーケンサー、新規遺伝子誘導システム、イメージングについてのワークショップも開催し、講師と参加者との間で熱心な議論が行われました。さらに、基礎生物学研究所は世界最大のベクター、突然変異体、完全長cDNA の供給センターとなっており、それらの入手方法についての説明も行われました。旅費、滞在費は自己負担でしたが、定員の3倍近い応募があり、全ての希望者を受け入れることができずに残念でした。参加者が帰国後、各国で実験技術を伝えていってくれることを期待したいと思います。昼食、夕食は連日異なったレストランに出向き、中日の岡崎城と八丁味噌工場へのエキスカーションと併せて、多くの参加者が日本の科学とともに文化も堪能し帰国されました。トレーニングコース後に開催された国際学会では国籍の異なるトレーニングコース参加者どうしが親しく議論する場面も良くみかけ、ヒメツリガネゴケコミュニティーの国際化にも大きな貢献ができたようです。(長谷部 光泰)
 
 Ever since the first NIBB International Practical Course, held in 2007, NIBB has continued to provide young scientists from around the world with opportunities to practice cutting-edge skills and to spend valuable time learning from experts in the field. For twenty years prior to 2007 NIBB held Bioscience Training Courses to offer these same opportunities, however they were attended mostly by the students of universities and institutes in Japan. The International Practical Course program was initiated to bring these goals to the world stage and offer the opportunities available at the Bioscience Training Courses to future researchers without regard to nationality. The Fourth International Practical Course was held from June 29th to July 3rd, 2009, focusing on techniques used in the biology of the moss Physcomitrella patens, an important model organism in plant biology.
 
 Seventeen students participated in the course, from Japan, Israel, Germany, Belgium, The Czech Republic, France, Sweden, Singapore, and India. They worked on learning new techniques and capitalized on the many chances to enjoy discussions with established researchers throughout the five days of the course.
 
 The course provided basic techniques including cultivation, observation of developmental processes at the cellular level, gene targeting, bioimaging, and bioinformatics. The course was taught, with the help of laboratory technicians, by Drs. Andrew C. Cuming (Univ. Leeds), Yasuko Kamisugi (Univ. Leeds), Tomoaki Nishiyama (Kanazawa Univ.), Tetsuya Kurata (JST), Yoshikatsu Sato (JST), Yuji Hiwatashi (NIBB), Minoru Kubo (JST), Takashi Murata (NIBB), Mitsuyasu Hasebe (NIBB). In-addition many postdoctoral fellows working at NIBB introduced the research they are perusing using P. patens and shared detailed research procedures with the participants.
 
 The laboratories used were provided by NIBB and equipped with the necessary instruments and tools as well as a good supply of material moss. We hope that the techniques and knowledge provided in this course will prove useful in the future work of all of the participants.
 
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Program

Program

June 28, Sun
17:00 Get together
 
June 29, Mon
09:00 Welcome address
(Kiyotaka Okada, Director General of NIBB)
 
09:05 Introduction of course and Physcomitrella patens Hasebe and Cuming
09:35 Spore germination Hiwatashi
  Culture and storage of protonemata and gametophores Hiwatashi
10:15 Observation of protonemata and gametophores Sato
  Analysis of protonema colony morphology Sato
  Regeneration of protonemata from excised leaves-1 Sato
11:05 Induction of gametangia and sporophytes Cuming and Kamisugi
  Sporangium collection and storage Cuming and Kamisugi
  Observation of antheridia and archegonia Cuming and Kamisugi
  Observation of sperms Cuming and Kamisugi
12:05 Lunch
13:00 PEG-mediated transformation Hiwatashi, Cuming and Kamisugi
17:00 Crosses of mutants defective in the sporophyte formation Cuming and Kamisugi
18:00 Dinner Kurata
20:00 Workshop 1: Next generation sequencer Kurata
 
June 30, Tue
09:00 PEG-mediated transformation Hiwatashi, Cuming and Kamisugi
13:00 Lunch
14:00 Genomic DNA extraction Sakakibara
  Chromatin immunoprecipitation Kurata
  RNA extraction Kurata
  Green PCR Kubo
15:00 DNA-gel-blot analysis Sakakibara
  RNA interference Kurata
  Artificial micro RNA Kurata
  Gene deletion Sakakibara
  Conditional deletion Kubo and Sato
  Dominant repression Kubo
  Overexpression Kubo
  Inducible expression Kubo
18:00 Dinner
20:00 Workshop 2: Targeting strategy Cuming and Kamisugi
 
July 1, Wed
09:00 PEG-mediated transformation Hiwatashi, Cuming and Kamisugi
11:00 How to use genome information Nishiyama
12:00 Lunch
  Free time / Seimar by Dr. Nir Ohad and Dr. Amal J. Johnston
 
July 2, Thu
09:00 How to use genome information Nishiyama
10:00 Analysis of protonema development and growth Sato and Hiwatashi
11:00 Observation of microtubules with indirect immunofluorescence microscopy Murata
  Light and electron microscopy of protonemata embedded with epoxy resin Murata
12:00 Lunch
13:00 DAPI, Hoechst33342, and PI staining of protonemata Murata
  Visualization of cell wall with calcoflur Murata
14:00 Live imaging Sato
  Cellular localization of fluorescent protein Sato
  Regeneration of protonemata from excised leaves-2 Sato
16:00 GUS staining Hiwatashi
17:00 How to use genome information Nishiyama
18:00 Dinner
20:00 Workshop 3: Bioimaging Sato, Murata, Cuming and Kamisugi
July 3, Fri
09:00 Measurement of DNA content with FACS Sato
10:00 Agrobacteria-mediated moss transformation:
Protonema and Agrobacteria Co-culture
Kurata
12:00 Lunch
13:00 Agrobacteria-mediated moss transformation:
Protonema and Agrobacteria Co-culture
Kurata
14:00 Hormone treatments Cuming and Kamisugi
15:00 Light response Sato
  Gravitropic response Sato
16:00 Closing discussion All lecturers
17:00 Farewell party