国際連携

EMBL - シンポジウム

Frontiers in Bioimaging

	Jan Ellenberg (EMBL, Heidelberg), Naoto Ueno (NIBB)
Organizers	Jan Ellenberg (EMBL, Heidelberg), Naoto Ueno (NIBB)
Venue	Okazaki Conference Center, Okazaki, Japan
Date	Mar. 22-23, 2006
Link	Symposium Website　（http://www.nibb.ac.jp/emblsymp/2nd/）
	Symposium Website　（http://www.nibb.ac.jp/emblsymp/2nd/）
Poster

Bioimaging is one of the chief subjects of the collaboration between NIBB and EMBL. This meeting was held with the aim of exchanging information and discussing emerging and innovative technologies including chemical probes, microscopes with new concepts, and image data processing.

Dr. Stelzer (EMBL) reported on the Selective Plane Illumination Microscopy (SPIM), which is a recently developed microscope that allows scientists to observe large-sized (up to a few millimeters) and even living specimens. Dr. Sedat (UCSF) talked about the advanced microscopic system “OMX”, the first practical implementation of structured illumination (SI) in which the grid is superimposed onto the sample to gain sharper images. Dr. Ellenberg talked about the genome-wide screening of mitosis-regulating genes called “MitoCheck” based on the mass image analysis of cultured cells combined with the disruption of genes with RNA interference.

Because of the high quality of the presentations and the lively discussions among the participants, this meeting was of significant importance for the field of bioimaging.

Program

Speaker	Affiliation	Time	Title
March 22nd
Registration
		8:40-	Registration
Opening Remarks
Yoshitaka Nagahama		9:05-9:15	Opening Remarks
Session1: Imaging diffusion & activity Chair: Yasushi Hiraoka
Atsushi Miyawaki	RIKEN, BRI, JAPAN	9:15-9:50	Visualization of the Spatial and Temporal Dynamics of Intracellular Signaling
Kai Johnsson	ISIC, EPFL, SWITZERLAND	9:50-10:25	Protein Chemistry in Living Cells
		10:25-10:50	Coffee break
Tom K Kerppola	Univ. Michigan, USA	10:50-11:25	Visualization of Protein Interactions and Modifications in Living Cells using Bimolecular Fluorescence Complementation Analysis
Philipe Bastiaens	EMBL, GERMANY	11:25-12:00	Reaction-Diffusion Cycles in the Spatial Organization of Cellular Signaling and Morphogenesis
		12:00-13:00	Lunch
Session2: Emerging technologies Chair: Peter T.C.So
Jennifer Lippincott-Schwartz	NIH, USA	13:00-13:35	Deciphering Protein Turnover, Topology and Transport in Living Cells
Masataka Kinjo	Hokkaido Univ., JAPAN	13:35-14:10	Analysis of Microenvironment of Nucleus Using Fluorescence Correlation Spectroscopy
Robert H. Singer	Yale Univ., USA	14:10-14:45	Following Single mRNAs in Living Cells
Akihiro Kusumi	Kyoto Univ., JAPAN	14:45-15:20	Single Molecule Tracking at the Cell Surface: Transient Signal Transduction by Engaged GPI-Anchored Receptors
		15:20-15:40	Coffee break
Session3: Bioluminescence Chair: Tom K Kerppola
Susumu Terakawa	Hamamatsu Med. Univ., JAPAN	15:40-16:15	Exocytosis and Endocytosis in Neuronal Cells Visualized with Ultra High NA Lens
Tarik Issad	INSERM, FRANCE	16:15-16:50	The Use of BRET (Bioluminescence Resonance Energy Transfer) for the Study of Tyrosine-kinase Receptors
Takeaki Ozawa	IMS, JAPAN	16:50-17:25	Methods for Identifying Organelle-Targeting Proteins
Session4: Networks & Screening Chair: Philippe Bastiaens
Tobias Meyer	Stanford, USA	17:25-18:00	Fluorescence Microscopy Approaches for Dissecting Signaling Networks
Jan Ellenberg	EMBL, GERMANY	18:00-18:35	Microscopy-Based RNAi Screening and Quantitative Imaging of Chromosome Structure to Identify and Define the Function of Mitotic Genes

19:00-21:00 Mixer and Poster session

Speaker	Affiliation	Time	Title
March 23rd
Session5: 3D imaging Chair: Jan Ellenberg / Ernst H.K. Stelzer
Ernst H.K.Stelzer	EMBL, GERMANY	9:00-9:35	Selective Plane Illumination Microscopy: Life Sciences Require the Third Dimension
John W Sedat	UCSF, USA	9:35-10:10	OMX, A Microscope Platform for the Future?
		10:10-10:30	Coffee break
Winfried Denk	MPI, GERMANY	10:30-11:05	Watching the Brain Compute and Tracing Its Wires: New Methods to Solve Old Riddles.
Haruo Kasai	NIPS, JAPAN	11:05-11:40	Dynamic Actin Organizations in Single Dendritic Spines of CA1 Pyramidal Neurons Studies with Two-Photon Photoactivation
Peter T.C. So	MIT, USA	11:40-12:15	Mechanotransduction: Understanding How Cells Sense Mechanical Signals with Novel Microscopic and Spectroscopic Tools
		12:15-13:00	Lunch
Session6: Mitosis/Trafficking Chair: Atsushi Miyawaki
Jason Swedlow	Dandee Univ., UK	13:00-13:35	Studies of Mitosis in Living Cells and Tissues
Yasushi Hiraoka	NICT, JAPAN	13:35-14:10	Live-cell Observation of Chromosome Dynamics in Fission Yeast
Yoshinori Ohsumi	NIBB, JAPAN	14:10-14:45	Molecular Dissection of Membrane Dynamics during Autophagy
		14:45-15:05	Coffee break
Session7: Organism model Chair: Jochen Wittbrodt / Naoto Ueno
Cornelis Weijer	Dandee Univ., UK	15:05-15:40	Chemotactic Cell Movement and Its Role in Morphogenesis
Damian Brunner	EMBL, GERMANY	16:15-16:15	Organizing Microtubules in Space and Time
Jochen Wittbrodt	EMBL, GERMANY	16:15-16:50	Individual Cell Migration as the Driving Force for Optic Vesicle Evagination
Kei Ito	Tokyo Univ.	16:50-17:25	Imaging the Structure of the Neural Circuits Using Molecular-Genetic Techniques
Minoru Tanaka	NIBB, JAPAN	17:25-18:00	A Gonadal Field Coordinates Germline and Somatic Precursors to Form a Gonadal Primordium