大学共同利用機関法人 自然科学研究機構

基礎生物学研究所

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EMBL - シンポジウム

Frontiers in Bioimaging

Organizers Jan Ellenberg (EMBL, Heidelberg), Naoto Ueno (NIBB) Frontiers in Bioimaging
Venue Okazaki Conference Center, Okazaki, Japan
Date Mar. 22-23, 2006
Link Symposium Website (http://www.nibb.ac.jp/emblsymp/2nd/)

embl2-1.jpg  embl2-2.jpg

 

 Bioimaging is one of the chief subjects of the collaboration between NIBB and EMBL. This meeting was held with the aim of exchanging information and discussing emerging and innovative technologies including chemical probes, microscopes with new concepts, and image data processing. 
 
 Dr. Stelzer (EMBL) reported on the Selective Plane Illumination Microscopy (SPIM), which is a recently developed microscope that allows scientists to observe large-sized (up to a few millimeters) and even living specimens. Dr. Sedat (UCSF) talked about the advanced microscopic system “OMX”, the first practical implementation of structured illumination (SI) in which the grid is superimposed onto the sample to gain sharper images. Dr. Ellenberg talked about the genome-wide screening of mitosis-regulating genes called “MitoCheck” based on the mass image analysis of cultured cells combined with the disruption of genes with RNA interference. 
 
 Because of the high quality of the presentations and the lively discussions among the participants, this meeting was of significant importance for the field of bioimaging.
Program
March 22nd
Speaker Affiliation Time Title
Registration
    8:40- Registration
Opening Remarks
Yoshitaka Nagahama   9:05-9:15 Opening Remarks
Session1: Imaging diffusion & activity     Chair: Yasushi Hiraoka
Atsushi Miyawaki RIKEN, BRI, JAPAN 9:15-9:50 Visualization of the Spatial and Temporal Dynamics of Intracellular Signaling
Kai Johnsson ISIC, EPFL, SWITZERLAND 9:50-10:25 Protein Chemistry in Living Cells
    10:25-10:50 Coffee break
Tom K Kerppola Univ. Michigan, USA 10:50-11:25 Visualization of Protein Interactions and Modifications in Living Cells using Bimolecular Fluorescence Complementation Analysis
Philipe Bastiaens EMBL, GERMANY 11:25-12:00 Reaction-Diffusion Cycles in the Spatial Organization of Cellular Signaling and Morphogenesis
    12:00-13:00 Lunch
Session2: Emerging technologies     Chair: Peter T.C.So
Jennifer Lippincott-Schwartz NIH, USA 13:00-13:35 Deciphering Protein Turnover, Topology and Transport in Living Cells
Masataka Kinjo Hokkaido Univ., JAPAN 13:35-14:10 Analysis of Microenvironment of Nucleus Using Fluorescence Correlation Spectroscopy
Robert H. Singer Yale Univ., USA 14:10-14:45 Following Single mRNAs in Living Cells
Akihiro Kusumi Kyoto Univ., JAPAN 14:45-15:20 Single Molecule Tracking at the Cell Surface: Transient Signal Transduction by Engaged GPI-Anchored Receptors
    15:20-15:40 Coffee break
Session3: Bioluminescence     Chair: Tom K Kerppola
Susumu Terakawa Hamamatsu Med. Univ., JAPAN 15:40-16:15 Exocytosis and Endocytosis in Neuronal Cells Visualized with Ultra High NA Lens
Tarik Issad INSERM, FRANCE 16:15-16:50 The Use of BRET (Bioluminescence Resonance Energy Transfer) for the Study of Tyrosine-kinase Receptors
Takeaki Ozawa IMS, JAPAN 16:50-17:25 Methods for Identifying Organelle-Targeting Proteins
Session4: Networks & Screening     Chair: Philippe Bastiaens
Tobias Meyer Stanford, USA 17:25-18:00 Fluorescence Microscopy Approaches for Dissecting Signaling Networks
Jan Ellenberg EMBL, GERMANY 18:00-18:35 Microscopy-Based RNAi Screening and Quantitative Imaging of Chromosome Structure to Identify and Define the Function of Mitotic Genes

19:00-21:00 Mixer and Poster session

March 23rd
Speaker Affiliation Time Title
Session5: 3D imaging     Chair: Jan Ellenberg / Ernst H.K. Stelzer
Ernst H.K.Stelzer EMBL, GERMANY 9:00-9:35 Selective Plane Illumination Microscopy: Life Sciences Require the Third Dimension
John W Sedat UCSF, USA 9:35-10:10 OMX, A Microscope Platform for the Future?
    10:10-10:30 Coffee break
Winfried Denk MPI, GERMANY 10:30-11:05 Watching the Brain Compute and Tracing Its Wires: New Methods to Solve Old Riddles.
Haruo Kasai NIPS, JAPAN 11:05-11:40 Dynamic Actin Organizations in Single Dendritic Spines of CA1 Pyramidal Neurons Studies with Two-Photon Photoactivation
Peter T.C. So MIT, USA 11:40-12:15 Mechanotransduction: Understanding How Cells Sense Mechanical Signals with Novel Microscopic and Spectroscopic Tools
    12:15-13:00 Lunch
Session6: Mitosis/Trafficking     Chair: Atsushi Miyawaki
Jason Swedlow Dandee Univ., UK 13:00-13:35 Studies of Mitosis in Living Cells and Tissues
Yasushi Hiraoka NICT, JAPAN 13:35-14:10 Live-cell Observation of Chromosome Dynamics in Fission Yeast
Yoshinori Ohsumi  NIBB, JAPAN 14:10-14:45 Molecular Dissection of Membrane Dynamics during Autophagy
    14:45-15:05 Coffee break
Session7: Organism model     Chair: Jochen Wittbrodt / Naoto Ueno
Cornelis Weijer Dandee Univ., UK 15:05-15:40 Chemotactic Cell Movement and Its Role in Morphogenesis
Damian Brunner EMBL, GERMANY 16:15-16:15 Organizing Microtubules in Space and Time
Jochen Wittbrodt EMBL, GERMANY 16:15-16:50 Individual Cell Migration as the Driving Force for Optic Vesicle Evagination
Kei Ito Tokyo Univ. 16:50-17:25 Imaging the Structure of the Neural Circuits Using Molecular-Genetic Techniques
Minoru Tanaka NIBB, JAPAN 17:25-18:00 A Gonadal Field Coordinates Germline and Somatic Precursors to Form a Gonadal Primordium