|Organizers||Jan Ellenberg (EMBL Heidelberg), Kuniaki Nagayama (OIB, Okazaki), Naoto Ueno (NIBB)|
|Venue||Okazaki Conference Center, Okazaki, Japan|
|Date||Apr. 20-22, 2009|
|Link||Symposium Website （http://www.nibb.ac.jp/emblsymp/index.html）|
This joint meeting was the second bioimaging-related meeting of the NIBB-EMBL joint meeting series following the first symposium “Frontiers of bioimaging”held in 2006, and aimed to overview the recent advance of bioimaging from the molecular to the organismal level. In contrast to the previous meeting in which light microscopy technologies focusing on cell functions and animal and plant development were discussed, in this meeting many cutting edge technologies of single molecule imaging at high resolutions with electron as well as light microscopy combined with quantitative analyses to capture molecular and cellular dynamics were presented. The talks illustrated the rapid advance of bioimaging during the last three years and outlined the future direction of bioimaging using new methodologies of electron and light microscopy, imagingbased large scale screening, and image processing for quantitative analysis of a diverse range of biological phenomena. Several short oral presentations were selected from posters and four young scholars including graduate students of EMBL participated in the meeting as a part of the student exchange program of the NIBB-EMBL collaboration, both of which further stimulated the meeting and contributed to the success of the symposium.
Comments from the Students
Xavier Heiligenstein (EMBL)
I found the content of the conference really interesting and of a great level. The comments, questions and discussion to me really facilitated going deeper in to each presentation. I got a great and deep overview of what light microscopy can offer us today in complement to electron microscopy and also enjoyed the external activities a lot, such as visiting the NIBB institute, its spectrophotometer and its brand new Light Sheet Microscope. Having the opportunity to give a short talk was also nice and lead to good feed-back during the poster session. Complementing this nice meeting, we had some great interaction with the Post-doc and Students and also greatly enjoyed the side parts of this symposium (a very nice party on Tuesday evening). And what to say about the excursion that lead us to see wonderful landscapes, cultural, and scientific Japanese pride. Again I would like to thank you again for such a nice welcome.
Peter Blattmann (EMBL)
I enjoyed the 9th NIBB-EMBL Symposium on Functional Imaging a lot. For me, it was an invaluable experience to come to Japan and attend this conference. I was amazed by the high standards of all the talks and discussions throughout the conference and profited a lot from them. The program was very well chosen and I think I received a good and interesting overview of the cutting edge research going on in this field. The hospitality with which we were received was outstanding and I felt very welcome at all times. However, for me personally, the greatest experience was to meet the Japanese students, post-docs and the speakers and thus to learn about the Science and technology conducted in Japan. I am very grateful to have received the opportunity to present both my poster and also a short talk at this conference. The following excursion was also very enjoyable and for the first time I experienced Japanese culture and mountains, which I liked very much. I am very glad, that this fruitful collaboration between NIBB and EMBL is continuing and look forward to further opportunities to interact with NIBB members. Thank you again so much for the hospitality and all your efforts!
|Apr. 20, Monday|
|09:20-09:30||Opening Remarks Kiyotaka Okada (Director-General, NIBB)|
|Structural Biology and Functional Imaging|
Stephen Cusack (EMBL Grenoble)
"Imaging Influenza Virus Polymerase in vitro and in vivo"
Michael Sattler (Helmholtz Zentrum München & TU München)
"Molecular Recognition and Dynamics of Protein Complexes in Splicing Regulation"
Masahide Kikkawa (Kyoto Univ.)
"Structure and Mechanism of Microtubule-Based Motors"
Kazuhiro Maeshima (NIG)
"How Is Genome DNA Compacted into a Mitotic Chromosome?"
|Electron Microscopy and Functional Imaging|
Kuniaki Nagayama (OIB)
"Nano-Bioimaging by Phase Contrast Electron Cro-tomography"
Achilleas Frangakis (Univ. Frankfurt)
"The Native Organization of Desmosomes"
Thomas Müller-Reichert (Max Planck Institute)
"Correlative Light Microscopy and Electron Tomography of the Early C. elegans Embryo"
Tokuko Haraguchi (KARC)
"Live CLEM: A New Approach for Observing Molecular Dynamics in High Resolution"
Xavier Heiligenstein (EMBL Heidelberg) *Short talk from posters
“High Resolution Map of the Spindle Pole Body from S. Cerevisiae by Electron Tomography of Frozen Hydrated Sections”
|Functional Imaging in Live Cells (I)|
Junichi Nabekura (NIPS)
"Microglia Surveillance of Synapses: in vivo Observation"
Akihiko Nakano (Univ. Tokyo / RIKEN ASI)
"High-Resolution Confocal Imaging to Visualize Dynamic Membrane Trafficking in Living Cells"
Amanda Cobos Correa (EMBL Heidelberg) *Short talk from posters
“New Fluorescent Tools to Image MMP-12 Activity in Lung Inflammation”
Nobuyuki Shiina (OIB) *Short talk from posters
“Visualization of Na+ in Neurons Deficient in RNG105, a Factor Involved in Dendritic Local Translation”
Naoshi Hattori (Univ. Tokyo) *Short talk from posters
“Functional Analysis of Arf GAP Family Proteins by Reconstitution of Split Fluorescent Proteins”
|17:30-18:30||Exhibition Viewing (Hamamatsu Photonics, Nikon, Olympus,Yokogawa Electric)|
|Apr. 21, Tuesday|
|Functional Imaging in Live Cells (II)|
Atsushi Miyawaki (RIKEN BSI / ERATO, JST)
"New Fluorescent Probes and New Perspectives in Bioscience"
Carsten Schultz (EMBL Heidelberg)
"Novel Probes to Analyze Cellular Events with Spatial Resolution "
|Functional Imaging in Whole Animals|
Ernst H. K. Stelzer (EMBL Heidelberg)
"Light Sheet Based Fluorescence Microscopy in Modern Biology"
Norihiro Sadato (NIPS)
"The Neural Basis of Social Reward and Decision-Making"
Toshihiko Fujimori (NIBB)
"Analysis of Cell Behaviors in Early Mouse Development"
Naoki Hida (Univ. Tokyo) *Short talk from posters
“Real-Time Imaging of Dual Interaction of Smad Proteins in Xenopus laevis Embryo Using Multicolor Luciferases”
Shigenori Nonaka (NIBB)
"Live Imaging of the Whole Mouse Embryo"
Yu Imuta (RIKEN CDB) *Short talk from posters
“Establishment of Novel Knock-in Mice for Both Live Imaging and Genetic Lineage Tracing”
Jan Ellenberg (EMBL Heidelberg)
"Phenotypic Profiling of the Human Genome by Time-Lapse Microscopy Reveals Genes with Functions in Cell Division, Survival or Migration"
Gohta Goshima (Nagoya Univ.)
"Genome-Wide RNAi Screen Identifies Genes Required for Mitotic Spindle Formation in Animal Cells"
Philipp J. Keller (EMBL Heidelberg)
"The Virtual Embryo: Automated Reconstruction of Vertebrate Development"
Peter Blattmann (EMBL Heidelberg) *Short talk from posters
“High Content Screening Microscopy Identifies Novel Proteins with a Putative Role in Cholesterol Metabolism and Homeostasis”
Annelie Wuensche (EMBL Heidelberg) *Short talk from posters
“High-Throughput Confocal Time-Lapse Imaging in Living Cells”
|Quantitative Image Processing|
Shuichi Onami (RIKEN ASI)
"Quantitative Cell Division Dynamics Analysis and Prediction of the Mechanism of Development in C. elegans"
Takeharu Nagai (Hokkaido Univ.)
"Measurement of Diffusion Coefficient of Biomolecules by Fluorescence Decay After Photostimulation of Photoswichable Fluorescent Proteins in Living Cells"
Matthias Weiss (DKFZ)
"Protein Motion in Living Cells - Anomalous Is Normal"
Akatsuki Kimura (NIG) *Short talk from posters
“A Model for Cell-Size-Dependent Spindle Elongation in C. elegansEmbryo”
Yuki Tsukada (Nagoya Univ.) *Short talk from posters
“Edge Evolution Tracking: Morphodynamic Analysis for Time-Lapse Images”
|18:30-20:00||Poster Session List of Poster titles|
|Apr. 22, Wednesday|
|New Techniques in Advanced Microscopy|
George Patterson (NIH)
"High Resolution Imaging of Protein Localization and Dynamics"
Hiro-o Hamaguchi (Univ. Tokyo)
"Can Raman Spectroscopy Image Life?"
John W. Sedat (UCSF)
"OMX, a New Microscope Platform for Increased Time and Spatial Resolution"
Satoshi Shimozono (Riken BSI) *Short talk from posters
“Diffusion of Large Molecules into Assembling Nuclei Revealed Using an Optical Highlighting Technique”
Kenta Saito (Hokkaido Univ.) *Short talk from posters
“A Mercury Arc Lamp-Based Multi-Color Confocal Real Time Imaging System for Cellular Structure and Function”
|11:20-12:20||Poidum Discussion: Next Challenges in Functional Imaging Across Biological Scales (All speakers)|
|12:20-12:30||Closing Remarks Jan Ellenberg (EMBL Heidelberg)|