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＜Original Articles＞
【2021】【2020】【2019】【2018】【2017】【2016】【2015】【2014】【2013】【2012】
【2011】【2010】【2009】【2008以前】
＜総説・著書等＞

～熱・温度の生物学的意義の解明を目指して～

 

～温度の生物学～

 

 
多くの生物は、熱ストレスから細胞を守る熱ショック応答機構（図1左) を持つ。生命熱動態研究室では温度と生物のつながりを明らかにするための一つの手段として、熱ショック転写因子（HSF）1に着目し、その温度感知機構と活性化のキネティクスを明らかにするため研究を始めている。現在は、メダカや、メダカ近縁種、ヒラメ、カエルなど複数の生物種を用いて、HSF1活性化の分子機構の解明に比較生物学的視点から取り組んでいる。 また、温度と生物のつながりを調べるうえで、生体物質の温度物性を知る必要がある。そこで、局所加熱しながら生体内標的細胞の温度計測ができる顕微鏡技術の開発も行っている。これまでに、2波長の蛍光強度の比から温度計測が可能な蛍光タンパク質プローブ (文献1) を応用した、高速生体温度イメージング系を構築（図2）した。これを用いて生体物質の熱物性解析に挑戦している。
文献1. Nakano M, Arai Y, Kotera I, Okabe K, Kamei Y, Nagai T. (2017) Genetically encoded ratiometric fluorescent thermometer with wide range and rapid response. PLoS One, 12(2), e0172344.
 

～局所遺伝子発現技術とその改良～

 

熱ショック応答を利用して、熱ショックプロモーターの下流に目的遺伝子を挿入して生物に導入することで、熱ショックによる目的遺伝子の発現誘導が可能になる。光学解析室では、顕微鏡により赤外レーザーを集光照射し生体内の単一細胞を温める（図1右）ことで、目的の細胞のみで目的遺伝子を発現誘導させる（操作する）ことができる技術を保有している。この手法（IR-LEGO法:文献4）を用いて、メダカ、ゼブラフィッシュ、シロイヌナズナなどのモデル生物に応用している。この技術により所外研究者との共同研究（文献2, 3）を多数実施している。一方で、現行のIR-LEGOにはいくつかの難しさがある。それを克服するために、前述の研究を応用してIR-LEGOの改良も進めている。
文献2. Okuyama T, Yokoi S, Abe H, Isoe Y, Suehiro Y, Imada H, Tanaka M, Kawasaki T, Yuba S, Taniguchi Y, Kamei Y, Okubo K, Shimada A, Naruse K, Takeda H, Oka Y, Kubo T and Takeuchi H. (2014) A neural mechanism underlying mating preferences for familiar individuals in medaka fish. Science, 343, 91-94.
文献3. Shimada A, Kawasishi T, Kaneko T, Yoshihara H, Yano T, Inohaya K, Kinoshita M, Kamei Y, Tamura K and Takeda H. (2013) Trunk exoskeleton in teleosts is mesodermal in origin. Nat. Commun., 4, 1639.
文献4. Kamei Y, Suzuki M, Watanabe K, Fujimori K, Kawasaki T, Deguchi T, Yoneda Y, Todo T, Takagi S, Funatsu T and Yuba S. (2009) Infrared laser-mediated gene induction in targeted single cells in vivo. Nat. Methods 6, 79-81.(Pubmed)

特任准教授　Specially appointed Associate Professor

特任助教　Specially appointed Assistant Professor

学振特別研究員　JSPS Research Fellow

博士研究員　Postdoctoral Researcher

技術支援員　Technical Assistants

木下 千恵　KINOSHITA, Chie
 

人材募集

・大学院生
総研大院生として、あるいは所属大学院からの派遣としての受け入れや、インターンシップ生としての受け入れ等も行っています。
・研究員
「生命熱動態研究室」ではポスドク募集中です。テーマ等の詳細はお問い合わせください。また、学振PDホストもしています。早めにご相談ください。申請書作成のサポートも致します。
詳しくはお問い合わせください。ykamei [at] nibb.ac.jp

【科研費】

・学術変革領域研究（A）（2020-2024）
「散乱・揺らぎ場の包括的理解と透視の科学（散乱透視学）」
　計画研究1「時空間光波シンセシスによる散乱透視基盤の構築」
　（代表者：的場修、分担者：亀井保博 他）
　計画研究6「生きた細胞や組織における散乱・揺らぎ計測と制御」
　（代表者：玉田洋介、分担者：坂本丞 他）
・基盤研究（B）（2020-2022）
「生物・細胞の3次元熱動態イメージング」
（代表者：亀井保博、分担者：野中茂紀）
・挑戦的研究（開拓）（2017-2022）
「生物・細胞にとっての熱とは何か？生体分子物性と進化的視点から考える」
（代表者：亀井保博）
・若手研究（2019-2021）
「熱ショック転写因子HSF1の温度感知機構の比較生物学的解析」
（代表者：坂本丞）
・研究活動スタート支援（2020-2021）
「逆遺伝学的手法による温度感受性変異株の創出と解析」
（代表者：友井拓実）
・特別研究員奨励費（2019-2021）
「時空間的に内在性遺伝子を誘導する人工転写因子システムの開発」
（JSPS特別研究員PD：鈴木美有紀）
・挑戦的研究（萌芽）（2021-2022）
「ホログラフィック光刺激・イメージングによる低分子ホルモンの全身移動制御と機能解明」
（代表者：玉田洋介、分担者：亀井保博）
・新学術領域研究・学術研究支援基盤形成（2016-2021）
「先端バイオイメージング支援プラットフォーム」支援課題
（代表者：狩野方伸、光学顕微鏡支援：L04 亀井保博）

【その他競争的資金】

・JST-CREST（2017-2022）
「オプトバイオロジーの開発による体液恒常性と血圧調節を司る脳内機構の解明」
（研究代表者：野田昌晴）
（分担課題「哺乳類用IR-LEGOの開発」主たる共同研究者：亀井保博）
・AMEDゲノム・データ基盤事業
　ナショナルバイオリソースプロジェクト（NBRP）中核拠点整備プログラム（2017-2021）「メダカ」支援課題
（拠点代表者：成瀬清、分担者：亀井保博 他）

【その他共同研究プロジェクト】

・自然科学研究機構 分野融合型研究（2019-2021）
「ヒトとメダカが見る世界：魚類の色覚は人類よりも優れているか？」
（研究代表者：深町昌司、分担者/予算執行責任者：亀井保博）日本女子大学深町研
・物質・デバイス領域共同研究拠点 展開共同研究B（2016-2021）
「生体内の単一細胞温度制御計測システムの開発」
（研究代表者：亀井保博、分担者：野中茂紀、坂本丞 他、受入教員：永井健治、小松崎民樹）大阪大学永井研

< Original Articles >（ご覧になりたい年をクリックすると遷移します）

【2021】【2020】【2019】【2018】【2017】【2016】【2015】【2014】【2013】
【2012】【2011】【2010】【2009】【2008以前】
 

< 総説・著書等 >（クリックすると遷移します）

• (80) Megumi Matsuo, Yasuhiro Kamei, Shoji Fukamachi. (2021)
• Behavioural red-light sensitivity in fish according to the optomotor response.
• R. Soc. Open Sci, 8, 210415
• (79)  Chih-Wei Zeng, Yasuhiro Kamei, Shuji Shigenobu, Jin-Chuan Sheu, Huai-Jen Tsai. (2021)
• Injury-induced Cavl-expressing cells at lesion rostral side play major roles in spinal cord regeneration.
• Open Biology, 11, 200304.（国際共同研究）
• (78)  Yuji Nishiike, Daichi Miyazoe, Rie Togawa, Keiko Yokoyama, Kiyoshi Nakasone, Masayoshi Miyata, Yukiko Kikuchi, Yasuhiro Kamei, Takeshi Todo, Tomoko Ishikawa-Fujiwara, Kaoru Ohno, Takeshi Usami, Yoshitaka Nagahama, Kataaki Okubo. (2021)
• Estrogen receptor 2b is the major determinant of sex-typical mating behavior and sexual preference in medaka.
• Current Biology, 31, 1-12. プレスリリース（東京大学）
• (77)  Yusuke Ashida, Yusuke Honma, Noriaki Miura, Takatoshi Shibuya, Hayao Kikuchi, Yosuke Tamada, Yasuhiro Kamei, Atsushi Matsuda, Masayuki Hattori. (2020)
• Imaging performance of microscopy adaptive-optics system using scene-based wavefront sensing.
• J. Biomed. Optics, 25, 123707.

• (76)  Akiko Kondow, Kiyoshi Ohnuma, Yasuhiro Kamei, Atsushi Taniguchi, Ryoma Bise, Yoichi Sato, Hisateru Yamaguchi, Shigenori Nonaka, Keiichiro Hashimoto. (2020)
• Light-sheet microscopy-based 3D single-cell tracking reveals a correlation between cell cycle and the start of endoderm cell internalization in early zebrafish development.
• Dev. Growth Differ. 62, 495-502.
• 
• This paper was selected as a cover art.
• (75)  Yuko Watanabe, Kazuho Okuya, Yuki Takada, Masato Kinoshita, Saori Yokoi, Shinichi Chisada, Yasuhiro Kamei, Hideki Tatsukawa, Naoyuki Yamamoto, Hideki Abe, Hisashi Hashimoto, Kiyotaka Hitomi. (2020)
  Gene disruption of medaka (Oryzias latipes) orthologue for mammalian tissue-type transglutaminase (TG2) causes movement retardation.
  J Biochem., 168, 213-222.
• (74)  Nodoka Kanazawa, Mayuko Goto, Yumi Harada, Chiaki Takimoto, Yuuka Sasaki, Tamaki Uchikawa, Yasuhiro Kamei, Megumi Matsuo, Shoji Fukamachi. (2020)
• Changes in a Cone Opsin Repertoire Affect Color-Dependent Social Behavior in Medaka but Not Behavioral Photosensitivity.
  Frontiers in Genetics, 11, fgene.2020.00801.
• (73)  Saori Yokoi, Kiyoshi Naruse, Yasuhiro Kamei, Satoshi Ansai, Masato Kinoshita, Mari Mito, Shintaro Iwasaki, Shuntaro Inoue, Teruhiro Okuyama, Shinichi Nakagawa, Larry J Young, Hideaki Takeuchi. (2020)
  Sexually dimorphic role of oxytocin in medaka mate choice.
  PNAS, 117, 4802-4808. プレスリリース（基礎生物学研究所）（北海道大学） （東北大学）（岡山大学）
• (72)  Yoshihiro Fujikawa, Tomoko Ishikawa‐Fujiwara, Tony Kuo, Norio Shinkai, Tatsuma Shoji, Takashi Kawasaki, Yasuhiro Kamei, Yoshiyuki Sakuraba, Ayuko Sato, Masato Kinoshita, Yoichi Gondo, Shunsuke Yuba, Tohru Tsujimura, Jun Sese, Takeshi Todo. (2020)
  Involvement of Rev1 in alkylating agent-induced loss of heterozygosity in Oryzias latipes.
  Genes Cells, 25, 124-138. 

• (71)  Makiko Kosugi, Shin-Ichiro Ozawa, Yuichiro Takahashi, Yasuhiro Kamei, Shigeru Itoh, Sakae Kudoh, Yasuhiro Kashino, Hiroyuki Koike. (2019)
  Red-shifted chlorophyll a bands allow uphill energy transfer to photosystem Ⅱ reaction centers in an aerial green alga, Prasiola crispa, harvested in Antarctica,
  Biochim Biophys Acta Bioenerg., 1861, 148139. 研究成果（アストロバイオロジーセンター）
• (70)  Gembu Abe, Toshinori Hayashi, Keigo Yoshida, Takafumi Yoshida, Hidehiro Kudoh, Joe Sakamoto, Ayumi Konishi, Yasuhiro Kamei, Takashi Takeuchi, Koji Tamura, Hitoshi Yokoyama. (2019)
  Insights regarding skin regeneration n non-amniote vertebrates: Skin regeneration without scar formation and potential step-up to a higher level of regeneration.
  Seminars in cell & developmental biology, 100,109-121. 
• (69)  Hironobu Ikehata, Toshio Mori, Yasuhiro Kamei, Thierry Douki, Jean Cadet, Masayuki Yamamoto. (2020)
  Wavelength- and tissue-dependent variations in the mutagenicity of cyclobutane pyrimidine dimers in mouse skin.
  Photochem Photobiol., php. 13159. 
• (68)  Yusuke Nakane, Ai Shinomiya, Wataru Ota, Keisuke Ikegami, Tsuyoshi Shimmura, Sho-Ichi Higashi, Yasuhiro Kamei, Takashi Yoshimura. (2019)
  Action spectrum for photoperiodic control of thyroid-stimulating hormone in Japanese quail (Coturnix japonica).
  PLoS One, 14, e0222106.
• (67)  Dukhyun Hwang, Satomi Wada, Azusa Takahashi, Hiroko Urawa, Yasuhiro Kamei, Shuh-ichi Nishikawa. (2019)
  Development of a Heat-inducible Gene Expression System using Female Gametophytes of Arabidopsis thaliana.
  Plant Cell Physiol., 60, 2564-2572. 
• (66)  Tomoya Uchimura, Seiji Hara, Takashi Yazawa, Yasuhiro Kamei, Takeshi Kitano. (2019)
  Involvement of heat shock proteins on the transcriptional regulation of corticotropin-releasing hormone in Medaka.
  Frontiers in Endocrinology, 10, 529.
• (65)  Tomoyuki Shikata, Fumio Takahashi, Hiroyo Nishide, Shuji Shigenobu, Yasuhiro Kamei, Setsuko Sakamoto, Kouki Yuasa, Yoshitaka Nishiyama, Yasuhiro Yamasaki and Ikuo Uchiyama. (2019)
  RNA-seq analysis reveals genes related to photoreception, nutrient uptake, and toxicity in a noxious red-tide raphidophyte Chattonella antiqua.
  Frontiers in Microbiology, 10, 1764. プレスリリース（基礎生物学研究所）（水産研究・教育機構）
• (64)  Fumiya Furukawa, Shin Hamasaki, Seiji Hara, Tomoya Uchimura, Eri Shiraishi, Natsumi Osafune, Hisanori Takagi, Takashi Yazawa, Yasuhiro Kamei, Takeshi Kitano. (2019)
  Heat shock factor 1 protects germ cell proliferation during early ovarian differentiation in medaka.
  Sci Rep, 9, 6927. プレスリリース（熊本大学）
• (63)  Tomoya Nakayama, Tsuyoshi Shimmura, Ai Shinomiya, Kousuke Okimura, Yusuke Takehana, Yuko Furukawa, Takayuki Shimo, Takumi Senga, Mana Nakatsukasa, Toshiya Nishimura, Minoru Tanaka, Kataaki Okubo, Yasuhiro Kamei, Kiyoshi Naruse & Takashi Yoshimura. (2019)
  Seasonal regulation of the lncRNA LDAIR modulates self-protective behaviours during the breeding season.
  Nature Ecology & Evolution, 3, 845-851.   プレスリリース（基礎生物学研究所）
• (62)  Erina Abe, Masaki Yasugi, Hideaki Takeuchi, Eiji Watanabe, Yasuhiro Kamei & Hirotsugu Yamamoto. (2019)
  Development of omnidirectional aerial display with aerial imaging by retro-reflection (AIRR) for behavioral biology experiments.
  Optical Review,  26, 221-229.
• (61)  Yumi Harada, Megumi Matsuo, Yasuhiro Kamei, Mayuko Goto, Shoji Fukamachi. (2019)
  Evolutionary history of the medaka long-wavelength sensitive genes and effects of artificial regression by gene loss on behavioural photosensitivity.
  Sci Rep, 25, 2726-2736.
• (60)  Shonan Amemiya, Taku Hibino, Takuya Minokawa, Kiyoshi Naruse, Yasuhiro Kamei, Isao Uemura, Masato Kiyomoto, Shin-ichi Hisanaga, Ritsu Kuraishi. (2019)
  Development of the coelomic cavities in larvae of the living isocrinid sea lily Metacrinus rotundus.
  Acta Zool., 96, 36-43.

• (59)  Makiko Kosugi, Fumino Maruo, Takeshi Inoue, Norio Kurosawa, Akinori Kawamata, Hiroyuki Koike, Yasuhiro Kamei, Sakae Kudoh, Satoshi Imura. (2018)
  A comparative study of wavelength-dependent photoinactivation in photosystem II of drought-tolerant photosynthetic organisms in Antarctica and the potential risks of photoinhibition in the habitat.
  Ann. Bot., 122, 1263-1278.
• (58)  Takahiro Hamada, Mako Yako, Marina Minegishi, Mayuko Sato, Yasuhiro Kamei, Yuki Yanagawa, Kiminori Toyooka, Yuichiro Watanabe, Ikuko Hara-Nishimura. (2018)
  Stress granule formation is induced by a threshold temperature rather than a temperature difference in Arabidopsis.
  J. Cell Sci., 131, jcs216051.
• (57)  Megumi Matsuo, Yoriko Ando, Yasuhiro Kamei, Shoji Fukamachi. (2018)
  A semi-automatic and quantitative method to evaluate behavioral photosensitivity in animals based on the optomotor response.(OMR)
  Biol Open, 7, bio033175. 
• (56)  Jun Tominaga, Yasutoshi Nakahara, Daisuke Horikawa, Ayumi Tanaka, Maki Kondo, Yasuhiro Kamei, Tsuneaki Takami, Wataru Sakamoto, Kazutoshi Unno, Atsushi Sakamoto, Hiroshi Shimada. (2018)
  Overexpression of the protein disulfide isomerase AtCYO1 in chloroplasts slows darkinduced senescence in Arabidopsis.
  BMC Plant Biol, 18, 80. 
• (55)  Yusuke Nagao, Hiroyuki Takada, Motohiro Miyadai, Tomoko Adachi, Ryoko Seki, Yasuhiro Kamei, Ikuyo Hara, Yoshihito Taniguchi, Kiyoshi Naruse, Masahiko Hibi, Robert N, Hisashi Hashimoto. (2018)
  Distinct interactions of Sox5 and Sox10 in fate specification of pigment cells in medaka and zebrafish.
  PLoS Genetics, 4, e1007260.
• (54)  Riho Hasugata, Shinichi Hayashi, Aiko Kawasumi-Kita, Joe Sakamoto, Yasuhiro Kamei and Hitoshi Yokoyama. (2018)
  Infrared laser-mediated single-cell-level gene induction in the regenerating tail of Xenopus laevis tadpoles.
  Cold Spring Harb Protoc.,  May 16 10.1101/pdb.prot101014  
• 
• This paper was selected as a cover art.
• (53)  Yasuhiro Tonoyama, Minori Shinya, Atsushi Toyoda, Takeshi Kitano, Atsunori Oga, Toshiyuki Nishimaki, Takafumi Katsumura, Hiroki Oota, Miles T Wan, Bill W P Yip, Mok O L Helen, Shinichi Chisada, Tomonori Deguchi, Doris W T Au, Kiyoshi Naruse, Yasuhiro Kamei, Yoshihito Taniguchi. (2018)
  Abnormal nuclear morphology is independent of longevity in a zmpste24-deficient fish model of Hutchinson-Gilford progeria syndrome (HGPS).
  Comp Biochem Physiol C Toxicol Pharmacol, 209, 54-62. 
• (52)  Yuko Watanabe, Eri Furukawa, Hideki Tatsukawa, Hisashi Hashimoto, Yasuhiro Kamei, Yoshihito Taniguchi, Kiyotaka Hitomi. (2018)
  Higher susceptibility to osmolality of the medaka (Oryzias latipes) mutants in orthologue genes of mammalian skin transglutaminases.
  Biosci Biotechnol Biochem. 82, 1165-1168. 
• (51)  Masatoshi Nakamoto, Yasushi Shibata, Kaoru Ohno, Takeshi Usami, Yasuhiro Kamei, Yoshihito Taniguchi, Takeshi Todo, Takashi Sakamoto, Graham Young, Penny Swanson, Kiyoshi Naruse, Yoshitaka Nagahama. (2018)
  Ovarion aromatase loss-of-function mutant medaka undergo ovary degeneration and partial female-to-male sex reversal after puberty.
  Mol. Cell. Endocrinol., 460, 104-122. 

• (50)  Tokiro Ishikawa, Makoto Kashima, Atsushi J. Nagano, Tomoko Ishikawa-Fujiwara, Yasuhiro Kamei, Takeshi Todo, Kazutoshi Mori. (2017)
  Unfolded protein response transducer IRE1-mediated signaling independent of XBP1 mRNA splicing is not required for growth and development of medaka fish.
  eLife, 6, e26845. 
• (49)  Tsuyoshi Shimmura, Tomoya Nakayama, Ai Shinomiya, Shoji Fukamachi, Masaki Yasugi, Eiji Watanabe, Takayuki Shimo, Takumi Senga, Toshiya Nishimura, Minoru Tanaka, Yasuhiro Kamei, Kiyoshi Naruse, Takashi Yoshimura. (2017)
  Dynamic plasticity in phototransduction regulates seasonal changes in color perception.
  Nat. Commun., 8412. プレスリリース（基礎生物学研究所）
• (48)  Masayuki Hattori, Yosuke Tamada, Takashi Murata, Shin Oya, Mitsuyasu Hasebe, Yutaka Hayano, Yasuhiro Kamei. (2017)
  Artificial testing targets with controllable blur for adaptive optics microscopes.
  Genome Biology Optical Engineering, 56, 080502.
• (47)  Yasuko Isoe, Ryohei Nakamura, Yasuhiro Kamei, Shigenori Nonaka, Teruhiro Okuyama, Atsushi Shimizu, Takeo Kubo, Hiroyuki Takeda, Hideaki Takeuchi.
• Clonal analysis of construction mechanism of the adult telencephalon mediated by post-hatch neurogenesiss in medaka fish.
  Mechanisms of Development, 145, S118. 
• (46)  Yoshihiro Morishita, Takayuki Suzuki, Hitoshi Yokoyama, Yasuhiro Kamei, Koji Tamura, Aiko Kawasumi.
• Quantitative and comparative analysis of tissue deformation dynamics for chick and Xenopus limb morphogenesis.
  Mechanisms of Development, 145, S40. 
• (45)  Tokiro Ishikawa, Takuya Toyama, Yuki Nakamura, Kentaro Tamada, Hitomi Shimizu, Satoshi Ninagawa, Tetsuya Okada, Yasuhiro Kamei, Tomoko Ishikawa-Fujiwara, Takeshi Todo, Eriko Aoyama, Masaharu Takigawa, Akihiro Harada, Kazutoshi Mori. (2017)
  UPR transducer BBF2H7 allows export of typeⅡ collagen in a cargo- and developmental stage-specific manner.
  J. Cell Biol., jcb. 201609100. プレスリリース（京都大学）
• (44)  Yosuke Tamada, Yutaka Hayano, Takashi Murata, Shin Oya, Yusuke Honma, Minoru Kanazawa, Noriaki Miura, Mitsuyasu Hasebe, Yasuhiro Kamei, Masayuki Hattori. (2017)
• Adaptive optical imaging through complex living plant cells.
• Proc. SPIE - The International Society for Optical Engineering 10251
• (43)  Masayuki Hattori, Yosuke Tamada, Shin Oya, Yutaka Hayano, Yasuhiro Kamei. (2017)
• Basic experiments of laser beam correcion by adaptive optics microscope for the accurate manipulation of biological tissues.
  Proc. SPIE - The International Society for Optical Engineering 10251
• (42)  Masahiro Nakano, Yoshiyuki Arai, Ippei Kotera, Kohki Okabe, Yasuhiro Kamei & Takeharu Nagai. (2017)
  Genetically encoded ratiometric fluorescent thermometer with wide range and rapid response.
  PLoS One, 12, e0172344. 
• (41)  Noriko Homma, Yumi Harada, Tamaki Uchikawa, Yasuhiro Kamei & Shoji Fukamachi. (2017)
  Protanopia (red color-blindness) in medaka: a simple system for producing color-blind fish and testing their spectral sensitivity.
  BMC Genetics, 18, 10. 

• (40)  Chih-Wei Zeng, Yasuhiro Kamei, Chih-Tien Wang, Huai-Jen Tsai. (2016).
  Subtypes of hypoxia-responsive cells differentiate into neurons in spinal cord of zebrafish embryos after hypoxic stress.
  Biol. Cell., 108, 357-377. 
• 
   
• This paper was selected as a cover art.
• (39)  Ryo Yokoyama, Hiroshi Yamamoto, Maki Kondo, Satomi Takeda, Kentaro Ifuku, Yoichiro Fukao, Yasuhiro Kamei, Mikio Nishimura, Toshiharu Shikanai. (2016).
  Grena-localized proteins, RIQ1 and RIQ2, optimize the dynamics of light-harvesting complex Ⅱ and grana stacking in Arabidopsis.
  Plant Cell, 28, 2261-2275.  
• (38)  Umi Utagawa, Shoichi Higashi, Yasuhiro Kamei, Shoji Fukamachi. (2016).
  Characterization of assortative mating in medaka: Mate discrimination cues and factors that blas sexual preference.
  Hormones and Behavior, 84, 9-17. 
• (37)  Saori Yokoi, Satoshi Ansai, Masato Kinoshita, Kiyoshi Naruse, Yasuhiro Kamei, Larry J Young, Teruhiro Okuyama, Hideaki Takeuchi. (2016).
  Mate-guarding behavior enhances male reproductive success via familarization with mating partners in medaka fish.
  Front Zool., 13, 21. 
• (36)  Miyuki Suzuki, Chiyo Takagi, Shinichirou Miura, Yuto Sakane, Makoto Suzuki, Tetsushi Sakuma, Naoaki Sakamoto, Tetsuya Endo, Yasuhiro Kamei, Yuko Sato, Hiroshi Kimura, Takashi Yamamoto, Naoto Ueno, Ken-ichi T. Suzuki. (2016)
  In vivo tracking of histone H3 lysine 9 acetylation in Xenopus laevis during tail regeneration
  Gene Cells, 21, 358-369. 
• (35)  Ryuichi Nishihama, Sakiko Ishida, Hiroko Urawa, Yasuhiro Kamei, Takayuki Kohchi. (2016).
• Conditional Gene Expression/Deletion Systems for Marchantia polymorpha Using its Own Heat-shock Promoter and the Cre/loxP-mediated Site-specific Recombination.
  Plant Cell Physiol., 27, 271-280.  

• (34)  Aiko Kawasumi-Kita, Toshinori Hayashi, Takuya Kobayashi, Chikashi Nagayama, Shinichi Hayashi, Yasuhiro Kamei, Yoshihiro Morishita, Takashi Takeuchi, Koji Tamura, Hitoshi Yokoyama. (2015)
  Application of local gene induction by infrared laser-mediated microscope and temperature stimulator to amphibian regeneration study.
  Dev. Growth Differ., 57, 601-613.  プレスリリース（基礎生物学研究所）
• (33)  Mikiko Nakashima, Manami Suzuki, Misako Saida, Yasuhiro Kamei, Md. Babul Hossain, Toshinobu Tokumoto. (2015)
  Cell-based assay of nongenomic actions of progestins revealed inhibitory G protein coupling to membrane progestin receptor α (mPRα)
  Steroids, 100, 21-26. 
• (32)  Saori Yokoi, Teruhiro Okuyama, Yasuhiro Kamei, Kiyoshi Naruse, Yoshihito Taniguchi, Satoshi Ansai, Masato Kinoshita, Larry J Young, Nobuaki Takemori, Takeo Kubo, Hideaki Takeuchi. (2015)
  An essential role of the arginine vasotocin system in mate-guarding behaviors in triadic relationships of medaka fish (Oryzias latipes)
  PLoS Genetics, 11, e1005009. 

• (31)  Shinichi Hayashi, Haruki Ochi, Hajime Ogino, Aiko Kawasumi, Yasuhiro Kamei, Koji Tamura, Hitoshi Yokoyama. (2014)
  Transcriptional regulators in the Hippo signaling pathway control organ growth in Xenopus tadpole tail regeneration.
  Dev. Biol., 396, 31-41. プレスリリース（基礎生物学研究所）
• (30)  Xing Fang, Naohiro Ide, Sho-Ichi Higashi, Yasuhiro Kamei, Tatsushi Toyooka, Yuko Ibuki, Kazuaki Kawai, Hiroshi Kasai, Keinosuke Okamoto, Sakae Arimoto-Kobayashi, Tomoe Negishi. (2014)
  Somatic cell mutations caused by 365 nm LED-UVA due to DNA double-strand breaks through oxidative damage.
  Photochem Photobiol Sci., 13, 1338-1346.
• (29)  Norikazu Murozumi, Ryo Nakashima, Toshiaki Hirai, Yasuhiro Kamei, Tomoko Ishikawa-Fujiwara, Takeshi Todo, Takeshi Kitano. (2014)
  Loss of follicle-stimulating hormone receptor function causes masculinization and suppression of ovarian development in genetically female medaka.
  Endocrinology, 155, 3136-3145. 
• (28)  Yosuke Tamada, Takashi Murata, Masayuki Hattori, Shin Oya, Yutaka Hayano, Yasuhiro Kamei, Mitsuyasu Hasebe. (2014) 
  Optical Property Analyses of Plant Cells for Adaptive Optics Microscopy.
  Int. J. Optomechatronics, 8, 89-99. 
• (27)  Yusuke Nagao, Takao Suzuki, Atsushi Shimizu, Tetsuaki Kimura, Ryoko Seki, Tomoko Adachi, Chikako Inoue, Yoshihiro Omae, Yasuhiro Kamei, Ikuyo Hara, Yoshihito Taniguchi, Kiyoshi Naruse, Yuko Wakamatsu, Robert N. Kelsh, Masahiko Hibi, Hisashi Hashimoto. (2014)
  Sox5 functions as a fate switch in medaka pigment cell development.
  PLoS Genet., 10, e1004246.  プレスリリース （基礎生物学研究所）
• (26)  Shinji Otozai, Tomoko Ishikawa-Fujiwara, Shoji Oda, Yasuhiro Kamei, Haruko Ryo, Ayuko Sato, Taisei Nomura, Hiroshi Mitani, Tohru Tsujimura, Hidenori Inohara, Takeshi Todo. (2014)
  p53-Dependent suppression of genome instability in germ cells.
  Mutat Res. Fundam. Mol. Mech. Mutagen, 760, 24-32.  
• (25)  Teruhiro Okuyama, Saori Yokoi, Hideki Abe, Yasuko Isoe, Yuji Suehiro, Haruka Imada, Minoru Tanaka, Takashi Kawasaki, Shunsuke Yuba, Yoshihito Taniguchi, Yasuhiro Kamei, Kataaki Okubo, Atsuko Shimada, Kiyoshi Naruse, Hiroyuki Takeda, Yoshitaka Oka, Takeo Kubo, Hideaki Takeuchi. (2014)
  A neural mechanism underlying mating preferences for familiar individuals in medaka fish.
  Science, 343, 91-94. プレスリリース （基礎生物学研究所）（東京大学）

• (24)  Teruhiro Okuyama, Yasuko Isoe, Masahito Hoki, Yuji Suehiro, Genki Yamagishi, Kiyoshi Naruse, Masato Kinoshita, Yasuhiro Kamei, Atushi Shimizu, Takeo Kubo, Hideaki Takeuchi. (2013)
  Controlled Cre/loxP site-specific recombination in the reveloping brain in medaka fish, Oryzias latipes.
  PLoS ONE, 8, e66597. 
• (23)  Eiji Kimura, Tomonori Deguchi, Yasuhiro Kamei, Wataru Shoji, Shunsuke Yuba, Jiro Hitomi. (2013)
  Application of infrared laser to the zebrafish vascular system: gene induction, tracing, and ablation of single endothelal cells.
  Arterioscler. Thromb. Vasc. Biol., 33, 1264-1270.  プレスリリース（基礎生物学研究所） （岩手医科大学）
• (22)  Atsuko Shimada, Toru Kawanishi, Takuya Kaneko, Hiroki Yoshihara, Tohru Yano, Keiji Inohaya, Masato Kinoshita, Yasuhiro Kamei, Koji Tamura, Hiroyuki Takeda. (2013)
  Trunk exoskeleton in teleosts is mesodermal in origin.  
  Nature Communications., 4, 1639.  プレスリリース （基礎生物学研究所）（東京大学）
• (21)  Tokiro Ishikawa, Tetsuya Okada, Tomoko Ishikawa-Fujiwara, Takeshi Todo, Yasuhiro Kamei, Shuji Shigenobu, Minoru Tanaka, Taro L. Saito, Jun Yoshimura, Shinichi Morishita, Atsushi Toyoda, Yoshiyuki Sakaki, Yoshihito Taniguchi, Shunichi Takeda, Kazutoshi Mori. (2013)
  ATF6α/β-3ediated adjustment of ER chaperone levels is essential for development of the notochord in medaka fish.
  Mol. Biol. Cell., 24, 1387-1395. 
• (20)  Hironobu Ikehata, Shoichi Higashi, Shingo Nakamura, Yasukazu Daigaku, Yoshiya Furusawa, Yasuhiro Kamei, Masakatsu Watanabe, Kazuo Yamamoto, Kotaro Hieda, Nobuo Munakata, Tetsuya Ono. (2013)
  Action spectrum analysis of UVR genotoxicity for skin: The border wavelengths between UVA and UVB can bring serious mutation loads to skin.
  J. Invest. Dermatol., 133, 1850-1856. 
• (19)  Tomoyuki Shikata, Shigeru Matsunaga, Mineo Iseki, Hiroyo Nishide, Sho-Ichi Higashi, Yasuhiro Kamei, Mineo Yamaguchi, Ian R. Jenkinson, Masakatsu Watanabe. (2013)
  Blue light regulates the rhythm of diurnal vertical migration in the raphidophyte red-tide alga Chattonella antiqua.
  J. Plankton Res., 35, 542-552. 
• (18) Kayo Kobayashi, Yasuhiro Kamei, Masato Kinoshita, Thomas Czerny, Minoru Tanaka. (2013)
  A heat-inducible CRE/LOXP gene induction system in medaka.
  Genesis, 51, 59-67, 

• (17)  Yasuda T, Oda S, Li Z, Kimori Y, Kamei Y, Ishikawa T, Todo T and Mitani H. (2012)
  Gamma-ray irradiation promotes premature meiosis of spontaneously differentiating testis-ova in the testis of p53-deficient medaka (Oryzias latipes).
  Cell Death Dis., 3, e395.
• (16)  Takeshi Kitano, Yuki Hayashi, Eri Shiraishi, Yasuhiro Kamei. (2012) 
  Estrogen rescues masculinization of genetically female medaka by exposure to cortisol or high temperature.
  Mol. Reprod. Dev., 79, 719-726.
• 
• This paper was selected as a cover art.
• (15)  Satoshi Ansai, Hiroshi Ochiai, Yuta Kanie, Yasuhiro Kamei, Yuki Gou, Takeshi Kitano, Takashi Yamamoto, Masato Kinoshita. (2012)
  Targeted disruption of exogenous EGFP gene in medaka using zinc-finger nucleases.
  Dev. Growth Differ., 54, 546-556.
• (14)  Haruo Masuyama, Masato Yamada, Yasuhiro Kamei, Tomoko Fujiwara-Ishikawa, Takeshi Todo, Yoshitaka Nagahama, Masaru Matsuda. (2012)
  Dmrt1 mutation causes a male-to-female sex reversal after the sex determination by Dmy in the medaka.
  Chromosome Res., 20, 163-176. 

• (13)  Tomoyuki Shikata, Mineo Iseki, Shigeru Matsunaga, Sho-ichi Higashi, Yasuhiro Kamei, Masakatsu Watanabe. (2011)
  Blue and red light-induced germination of resting spores in the red-tide diatom Leptocylindrus danicus.
  Photochem. Photobiol., 87, 590-597. 

• (12)  Tomoko Ishikawa#, Yasuhiro Kamei#, Shinji Otozai, Jinhyong Kim, Ayuko Sato, Yoshikazu Kuwahara, Minoru Tanaka, Tomonori Deguchi, Hidenori Inohara, Tohru Tsujimura, Takeshi Todo. (2010)
  High-resolution melting curve analysis for rapid detection of mutations in a Medaka TILLING library.
  BMC Mol. Biol, 11, 70. (#;equally contribution) 
• (11)  Yuki Hayashi, Hiroshi Kobira, Toshiya Yamaguchi, Eri Shiraishi, Takashi Yazawa, Toshiaki Hirai, Yasuhiro Kamei, Takeshi Kitano. (2010)
  High temperature causes masculinization of genetically female medaka by elevation of cortisol.
  Mol. Reprod. Dev., 77, 679-686.
• 
• This paper was selected as a cover art.
• (10)  Shoji Oda, Sachi Mikami, Yusuke Urushihara, Yasuhiko Murata, Yasuhiro Kamei, Tomonori Deguchi, Takeshi Kitano, Kazuhiro E. Fujimori, Shunsuke Yuba, Takeshi Todo, Hiroshi Mitani. (2010)
  Identification of a functional medaka heat shock promoter and characterization of its ability to induce exogenous gene expression in medaka in vitro and in vivo.
  Zool. Sci., 27, 410-415. 

• (9)  Tomonori Deguchi, Mariko Itoh, Hiroko Urawa, Tomohiro Matsumoto, Sohei Nakayama, Takashi Kawasaki, Takeshi Kitano, Shoji Oda, Hiroshi Mitani, Taku Takahashi, Takeshi Todo, Junichi Sato, Kiyotaka Okada, Kohei Hatta, Shunsuke Yuba, Yasuhiro Kamei. (2009)
  Infrared laser-mediated local gene induction in medaka, zebrafish and Arabidopsis thaliana.  
  Dev. Growth Differ., 51, 769-775. (corresponding author) 
• (8)  Yasuhiro Kamei*, Motoshi Suzuki, Kenjiro Watanabe, Kazuhiro Fujimori, Takashi Kawasaki, Tomonori Deguchi, Yoshihiro Yoneda, Takeshi Todo, Shin Takagi, Takashi Funatsu, Shunsuke Yuba. (2009)
  Infrared laser-mediated gene induction in targeted single cell in vivo. 
  Nature Methods, 6, 79-81. (2009) (corresponding author) 

• (7)  Junya Tomida, Yuji Masuda, Hidekazu Hiroaki, Tomoko Ishikawa, Ihnyoung Song, Toshiki Tsurimoto, Satoshi Tateishi, Tadahiro Shiomi, Yasuhiro Kamei, Jinhyeong Kim, Kenji Kamiya, Cyrus Vaziri, Haruo Ohmori, Takeshi Todo. (2008)
  DNA damage-induced ubiquitylation of RFC2 subunit of replication factor C complex.
  J. Biol. Chem., 283, 9071-9079.
• (6)  Yasuhiro Kamei*, Junji Itou, Shoji Oda, Mami Masui, Jin-Hyeong Kim, Tomoko Ishikawa, Shunsuke Yuba, Masato Kinoshita, Hiroshi Mitani, Takeshi Todo. (2007)
  Development of a convenient in vitro fertilization method using interspecific hybrids between Oryzias latipes and Oryzias curvinotus.
  Dev. Growth Differ., 49, 721-730. (corresponding author) 
• (5)  Yoshihito Taniguchi, Shunichi Takeda, Makoto Furutani-Seiki, Yasuhiro Kamei, Takeshi Todo, Takao Sasado, Tomonori Deguchi, Hisato Kondoh, Josine Mudde, Mitsuyoshi Yamazoe, Masayuki Hidaka, Hiroshi Mitani, Atsushi Toyoda, Yoshiyuki Sakaki, Ronald H. A. Plasterk, Edwin Cuppen. (2006)
  Generation of medaka gene knockout models by target-selected mutagenesis.
  Genome Biology, 7, R116. 
• (4)  Yukihiro Kondo, Junpei Kondoh, Daisuke Hayashi, Tadanobu Ban, Masatoshi Takagi, Yasuhiro Kamei, Lyuji Tsuji, Jiyoong Kim, Yoshihiro Yoneda. (2002)
  Molecular cloning of one isotype of human lamina-associated polypeptide 1s and a topological analysis using its deletion mutants.
  Biochem. Biophys. Res. Commun., 294 (4), 770-778. 
• (3)  Yuri Kobayashi, Tomoko Ishikawa, Jun Hirayama, Hiromi Daiyasu, Satoru Kanai, Hiroyuki Toh, Itsuki Fukuda, Tohru Tsujimura, Nobuyuki Terada, Yasuhiro Kamei, Shunsuke Yuba, Shigenori Iwai, Takeshi Todo. (2000)
  Molecular analysis of zebrafish photolyase/cryptochrome family: two types of cryptochromes present in zebrafish.
  Genes Cells, 5 (9), 725-738. 
• (2)  Yasuhiro Kamei, Shunsuke Yuba, Tatsuo Nakayama, Yoshihiro Yoneda. (1999)
  Three distinct classes of the α-subunit of the nuclear pore-targeting complex (importin-α) are differentially expressed in adult mouse tissues.
  J. Histochem. Cytochem., 47 (3), 363-372. 
• (1)  Naoko Imamoto, Yasuhiro Kamei, Yoshihiro Yoneda. (1998)
  Nuclear transport factors: function, behavior and interaction.
  Eur. J. Histochem, 42 (1), 9-20. 

• 23) 【著書】 亀井保博、坂本丞他（加藤輝、小山宏史編）、「達人に訊く＜バイオ画像取得と定量解析Q＆A-顕微鏡の設定からImageJによる解析・自動化まで＞」、実験医学別冊（羊土社）、220ページ、2021年3月
• 
• 22) 【総説】 坂本丞、永井健治、亀井保博「蛍光タンパク質温度プローブとその活用」月刊細胞 52, 41-45.  2020年11月
• 21) 【総説】 亀井保博、「最近のイメージング領域のホットトピック」実験医学 37, 3151-57.  2019年11月
• 20) 【著書（編集）】 Kenji Murata, Masato Kinoshita, Kiyoshi Naruse, Minoru Tanaka and Yasuhiro Kamei (Edit), Medaka: Biology, Management, and Experimental Protocols, Volume 2, ISBN: 978-1-119-57529-0, (2019), Wiley-Blackwell 360 Pages.
• 
• 19) 【総説】 竹内秀明、深町昌司、 亀井保博、「 総論：メダカの行動学研究の最先端 ──メダカはなぜ豊かな色彩を持つのか？」生物の科学 遺伝 72, 608-610.  2018年10月
• 18) 【総説】 亀井保博、「光と熱ショック応答を利用した生体内遺伝子発現誘導技術」Optics & Photonics Japan 2017 講演予稿集 1aES2  2017年11月 
• 17) 【総説】 坂本丞、亀井保博、「赤外レーザーによる遺伝子発現の操作技術」生体の科学 68, 500-501.  2017年10月
• 16) 【総説】 玉田洋介、早野裕、亀井保博、服部雅之、「生組織深部の超解像イメージングへ：光の乱れをアクティブに補正する補償光学」顕微鏡 52, 72-76.  2017年9月
• 15) 【総説】 亀井保博、「光を使って生体内の単一細胞に遺伝子発現を誘導する技術とその共同利用による応用研究の紹介」月刊海洋  557, 208-213.  2017年4月
• 14) 【総説】 亀井保博、「光を使った生物研究技術(IR-LEGO法)とその応用例」日本プランクトン学会報  64, 77-80.  2017年2月
• 13) 【総説】 斎田（谷口）美佐子、亀井保博、「IR-LEGO技術の紹介とその利用方法」化学と生物（表紙） 53, 580-585.  2015年9月
• 
• 12) 【総説】 亀井保博、「赤外レーザによる生体内局所遺伝子発現法 (IR-LEGO)」レーザ加工学会講演論文集 = Proceedings of the 81st Laser Materials Processing Conference 第81回   81.  2014年7月
• 11) 【総説】 内川珠樹、紫加田知幸、亀井保博、「大型分光照射装置「岡崎大型スペクトログラフ」紹介とプランクトン 研究への応用例」日本プランクトン学会報   61, 1-4.  2014年3月
• 10) 【総説】 亀井保博、「光で遺伝子発現を操作する : 局所遺伝子発現法 (IR-LEGO) の原理と応用」CYTOMETRY RESEARCH   24, 21-25.  2014年
• 9)【総説】成瀬清、木村哲晃、笹土隆雄、亀井保博、「メダカバイオリソースプロジェクトの展望とその生物機能解析への応用」水産育種  42, 1-10.  2012年12月
• 8)【著書】（共著） Denny P and Kole C edit, (Part; Kimura T, Kamei Y, Takehana Y, Sasado T, Naruse K), Medaka genomics and the methods and resources for decoding genomic functions, Springer, 28th Sep. 2012
• 7)【著書】（共著）　小幡裕一、城石俊彦、芹川忠夫、田中啓二、米川博通（編集）、（分担部分）亀井保博、「3 赤外レーザーを用いた局所的な生体内遺伝子発現法のメダカへの応用」、モデル動物利用マニュアル・生物機能モデルと新しいリソース・リソースツール、エル・アイ・シー[LIC co., Bunkyo-Tokyo, Japan], 579-582. (2011)
• 6)【総説】清水厚志、亀井保博、清水信義「メダカを用いたヒト疾患モデル」比較内分泌学, 36, 286-292. (2010)
• 5)【総説】浦和博子、出口友則、木村英二、伊藤真理子、鈴木基史、岡田清孝、八田公平、高木新、弓場俊輔、亀井保博、「赤外レーザーによる局所遺伝子発現誘導法」比較内分泌学, 36, 217-221. (2010)
• 4)【総説】貴田亨、石川智子、清水厚志、亀井保博、藤堂剛、三輪正直、清水信義、「TILLING法による遺伝子変異メダカの作製」比較内分泌学, 36, 154-158. (2010)
• 3)【総説】亀井保博、清水厚志、清水信義、「メダカ変異体を使った個体レベルのゲノム研究の展望」比較内分泌学, 36, 66-68. (2010)
• 2)【著書】（共著）Kinoshita M, Murata K, Naruse K and Tanaka M edit, Medaka: Biology, management and experimental protocols. [Wiley-Blackwell, Ames, Iowa]. (2009) parts; [Oda S and Kamei Y (Chapter editor), Medaka management (Chapter 2), 31-66; Hashimoto H and Kamei Y, Shipping (section 4.1), 101-103; Kamei Y, Infertile mating method for collecting unfertilized eggs (Column 4.1), 115-116; Kinoshita M and Kamei Y (Chapter editor), Transgenesis (Chapter 7), 277-296; Ishikawa T and Kamei Y, Cell culture from medaka embryo (section 10.1), 345-349;  Kamei Y, Tilling (gene knockout)(section 10.8), 374-380]
• 1)【著書】（共著）Volff J N edit, Vertebrate genomes. genome dyn., [Karger, Basel], vol 2, 165-182. (2006) (part: Mitani H, Kamei Y, Fukamachi S, Oda S, Sasaki T, Asakawa S, Todo T and Shimizu N. Medaka genome: Why we need the multiple fish models in vertebrate functional genomics.)