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National Institutes of Natural Sciences

National Institute for Basic Biology

International Cooperation

Practical Courses - Conference

The 10th NIBB International Practical Course "Genome Editing and Imaging of Fish and Amphibians"

Organizers Kiyoshi Naruse (NIBB/NBRP-Medaka/IBBP)
Hajime Ogino (Hiroshima Univ./NBRP-Xenopus)
Yasuhiro Kamei (NIBB/ABiS)
Ken-ichi T. Suzuki (Hirosima Univ./NIBB/iNewt)
Toshinori Hayashi (Tottori Univ./iNewt)
Satoshi Ansai (NIBB)
Kiyoshi Tatematsu (NIBB)
Pung-Pung Hwang (Academia Sinica, Taiwan)
Venue National Institute for Basic Biology (NIBB), Okazaki, Japan
Date Sep. 20-29, 2018
Link Website (http://www.nibb.ac.jp/course10/)
Poster The 10th NIBB International Practical Course
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 The 10th NIBB International Practical Course was held from 20th to 29th September, 2018. We received 13 applications and selected a total of nine participants joined the course. Three of them were Japanese and the other six were from the UK, Colombia, Nepal, Korea, China and Taiwan respectively.
 The title of the course was “Genome Editing and Imaging of Fish and Amphibians”. Medaka, Xenopus and iberian ribbed newt were used in this course. The course itself contained segments concerning “Gene knock-out and knock-in by the CRISPR-Cas9 method”, “Imaging analysis using a light-sheet microscope and a confocal microscope”, “Gene expression induction using the IR-LEGO system”, “Cryopreservation of medaka sperm” and “Optomoter response analysis of medaka using large spectrograph and data analysis of the response” among others. 10 open seminars by invited speakers coming from Japan and abroad were held. Dr. Pung-Pung Hwang (Institute of Cellular and Organismic Biology (ICOS), Academia Sinica, Taiwan), Dr. Lazaro Centanin (Centre for Organismal Studies, University of Heidelberg, Germany) and Dr. Takuji Sugiura (Research Institute of Molecular Pathology, Austria) were invited to lecture, and they conducted both seminars and course lectures.
 The 11th NIBB International Practical Course co-organized by NIBB and Academia Sinica will be held in Taipei, Taiwan in 2020. To discuss several matters related to the next course, six researchers including Dr. Hwang visited NIBB from ICOS during the course. They visited several laboratories and facilities during their visit and presented their research during the NIBB Special Seminar.
 Moving into the future, we hope that the NIBB International Practical Course will be an opportunity to start as well as advance international collaborations and cooperation at various level. Finally, researchers from abroad need financial support for their travel and living expenses to participate in this course. As such, we realized the necessity of reviewing certain aspects of course administration (such as publicizing the course earlier than before) in order to make it easier for foreign applicants to receive financial support from various funding agencies.
 
On behalf of the organizers
Kiyoshi Naruse (NIBB)
Hajime Ogino (Amphibian Research Center, Hiroshima University)
 
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Program
Thu, September 20th, 2018
11:00                    Registration opens
13:30                    Opening Remarks
14:00 – 15:00       Orientation, Guidance, and Animal welfare lecture/Dr. Kiyoshi Naruse
15:00 – 18:00       Genome Editing lecture and experiment (Medaka)/Dr. Satoshi Ansai
18:00 – 20:00       Welcome Party
   
   
Fri, September 21st, 2018
  9:00 – 12:00       Microinjection lecture and experiment (Medaka)/Dr. Satoshi Ansai
13:30 – 17:00       Construction of Guide RNA/Dr. Satoshi Ansai
17:00 – 18:00       Seminar presented by Dr. Pung-Pung Hwang (Academia Sinica, Taiwan)
18:00 – 20:00       Social Hour (Presentation from participants and discussion with lecturers)
   
   
Sat, September 22nd, 2018
  9:00 – 12:00       Microinjection experiment (Medaka)
13:30 – 15:30       Microinjection experiment (Medaka)
15:30 – 17:00       Husbandry lecture/Dr. Kiyoshi Naruse
17:00 – 19:00       Seminar presented by Dr. Hitoshi Yokoyama (Hirosaki University)
                             Seminar presented by Dr. Satoshi Ansai (NIBB)
   
    
Sun, September 23rd, 2018
No set program  
   
   
Mon, September 24th, 2018
No set program  
   
   
Tue, September 25th, 2018
  9:00 – 12:00       Xenopus lecture/Dr. Hajime Ogino
                             Genome Editing lecture (Xenopus)/Dr. Ken-ichi T. Suzuki
13:30 – 17:00       Microinjection experiment (Xenopus)/Drs. Hajime Ogino and Ken-ichi T. Suzuki
17:00 – 18:00       Seminar presented by Dr. Takeshi Igawa (Hiroshima University)
18:00 – 20:00       Social Hour (Presentation from participants and discussion with lecturers)
   
   
Wed, September 26th, 2018
  8:40 – 9:00         Xenopus egg selection
  9:00 – 12:30       IR-LEGO lecture/Dr. Yasuhiro Kamei
                             Light-sheet LSM lecture/Dr. Shigenori Nonaka
14:00 – 17:30       IR-LEGO operation (Medaka and Xenopus)
                             Observation using Light-sheet LSM (Medaka and Xenopus)
17:30 – 19:30       Seminar presented by Dr. Haruki Ochi (Yamagata University)
                             Seminar presented by Dr. Toshinori Hayashi (Tottori University / iNewt)
   
   
Thu, September 27th, 2018
  9:00 – 12:00       Lecture about Regeneration Study using Amphibians/Dr. Toshinori Hayashi
                             Regeneration Operation using Xenopus/Dr. Aiko Kawasumi
13:30 – 17:00       Regeneration Operation using Iberian Ribbed Newt/Dr. Toshinori Hayashi
                             Observation of IR-LEGO treated embryos
17:00 – 19:00       Seminar presented by Dr. Lazaro Centanin (Heidelberg University, Germany)
                             Seminar presented by Dr. Takuji Sugiura (IMP, Austria)
   
   
Fri, September 28th, 2018
  9:00 – 12:00       Lecture and experiment about Imaging using LSM/Dr. Shigenori Nonaka
13:30 – 17:00       Cryopreservation lecture and experiment/Dr. Kiyoshi Naruse
17:00 – 19:00       Seminar presented by Dr. Shinya Komoto (OIST)
                             Seminar presented by Dr. Asano Ishikawa (National Institute of Genetics)
18:00– 20:00        Farewell Party (Group discussion about future research and feedback on the course)
   
   
Sat, September 29th, 2018
  8:40 – 9:00        Observation of CRSPR/Cas9 Injected Xenopus
  9:00 – 12:00       Lecture and experiment about Behavior Assay/Dr. Shoji Fukamachi
13:30 – 15:00       Data Analysis of Behavior Assay/Dr. Yoriko Ando
15:30                    Closing Ceremony