1st "Developmental Genetics of Zebrafish and Medaka"

Organizers	Shinji Takada (NIBB) Shin-ichi Higashijima (NIPS) Koichi Kawakami (NIG) Hitoshi Okamoto (RIKEN) Hiroyuki Takeda (Univ. of Tokyo) Minoru Tanaka (NIBB)
Venue	National Institute for Basic Biology
Date	Jan. 15-24, 2007
Link	Website　（http://www.nibb.ac.jp/course/past/1st/）
Poster

The 1st NIBB International Practical Course was held from Jan. 15th to 24th, 2007. It was our first attempt to approach young scientists around the world instead of limiting to the domestic applicants till the previous year.
10 participants were highly selected, consisting of various nationalities; 1 Indian, 3 Hong Konger (Chinese), 3 Taiwanese, 1 Chinese, and 2 Japanese. They did excellent works with enthusiasm and had fruitful discussions throughout 10 days.
The course provided the study of developmental genetics of Zebrafish and Medaka with the high quality skills by the distinguished lecturer from inside and outside of NIBB. Dr. Kiyoshi Naruse (The Tokyo Univ.), Dr. Hironori Wada and Dr. Tomomi Sato (RIKEN Brain Science Institute), Dr. Karuna Sampath (The National Univ. Singapore) were invited as the lecturer.
It is a great opportunity to learn high skills and communicate with scientists in the well-prepared facility. The next course will be in March 2008. Stay tuned for further updates in the fall. 

Monday, January 15th, 2007
 
09:00-09:50 Breakfast
10:00-10:30 Registration (Meeting Room at NIBB B1 Floor)
10:30-11:00 Opening Remarks
11:00-12:00 Lecture on exp. 1 “BAC homologous recombination techniques for zebrafish transgenesis” 
12:00-13:00 Lunch
13:00-18:00 Exp. 1 (PCR for targeting DNA, Purification and precipitation of targeting DNA)
19:00-20:30 Welcome Party
 
Tuesday, January 16th, 2007
 
08:00-08:50 Breakfast
09:00-11:00 Exp. 1 (Demonstration of injection)
11:00-12:00 Exp. 1 (Agarose gel electrophoresis of the purified DNA)
12:00-13:00 Lunch
13:00-15:00 Exp. 1 (Homologus recombination and transformation)
15:00-18:00 Lecture on exp. 2 “How to clone your favorite medaka mutants”
19:00-20:30 Dinner
 
Wednesday, January 17th, 2007
 
08:00-08:50 Breakfast
09:00-12:00 Exp. 2 (PCR with the balked samples by M markers, electrosoresis and determination of candidate LG)
12:00-13:00 Lunch
13:00-16:00 Exp. 2 continued
16:00-17:00 Exp. 1 (Colony Picking up)
17:00-18:00 Evening Lecture I (S. Takada)
19:00-20:30 Dinner
 
Thursday, January 18th, 2007
 
08:00-08:50 Breakfast
09:00-12:00 Exp. 1 (PCR for Checking) and Exp. 2 (Determination of the map position of the mutant locus and the most adjacent DNA markers of the mutant locus)
12:00-13:00 Lunch
13:00-17:00 Exp. 2 (Identification of responsible region of mutant phenotype on the medaka draft genome)
17:00-18:00 Evening Lecture II (T. Iguchi)
19:00-20:30 Dinner
 
Friday, January 19th, 2007
 
08:00-08:50 Breakfast
09:00-12:00 Exp. 1 (BAC Injection into cytoplasm of zebrafish embryos)
12:00-13:00 Lunch
13:00-17:30 Exp. 2 (Narrowing down the responsible region with DNA markers and identification mutation site by DNA sequencing) 
17:30-18:30 Evening Lecture III (H. Takeda)
19:00-20:30 Dinner
 
Saturday, January 20th, 2007
 
Lab is open for participants. 
09:00-17:00 Exp. 1 (Injection and observation)
 
Sunday, January 21st, 2007
 
Lab is closed.
 
Monday, January 22nd, 2007
 
08:00-08:50 Breakfast
09:00-10:00 Lecture on exp. 3 “Tracing cell lineages with a fluorescent protein Kaede”
10:00-12:00 Exp. 3 (Kaede injection into zebrafish embryos)
12:00-13:00 Lunch
13:00-17:00 Exp. 3 (UV Irradiation)
17:00-18:00 Evening Lecture III (Y. Nagahama)
19:00-20:30 Dinner
 
Tuesday, January 23rd, 2007
 
08:00-08:50 Breakfast
09:00-12:00 Exp. 3 (Observation of injected embryos under Microscopes)
12:00-13:00 Lunch
13:00-16:00 Exp. 3 continued (Observation under Microscopes)
16:00-17:30 NIBB Seminar (K. Sampath)
18:00-20:00 Farewell Party
 
Wednesday, January 24th, 2007
 
08:00-08:50 Breakfast
09:00-11:50 Lecture (K. Sampath)
11:50-12:00 Closing Remarks
12:00-13:00 Lunch