National Institute for Basic Biology
| Organizers | Shinji Takada (NIBB) Hitoshi Okamoto (RIKEN BSI) Hiroyuki Takeda (Univ. of Tokyo) Masahiko Hibi (RIKEN CDB) Shin-ichi Higashijima (NIPS) Koichi Kawakami (NIG) Minoru Tanaka (NIBB) Kiyoshi Naruse (NIBB) |
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| Venue | National Institute for Basic Biology |
| Date | Mar. 3-12, 2008 |
| Link | Website （http://www.nibb.ac.jp/course/past/2nd/） |
| Poster |
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The 2nd NIBB International Practical Course was held from March 3rd to 12th, 2008. Just as the 1st course, which was held in January 2007, the 2nd course still focused on fish biology.
12 participants were highly selected, consisting of variousn nationalities;
1 Australian, 3 Hong Konger, 2 Taiwanese, 1 German, 2 Chinese, 1 Korean,
1 Norwegian, and 1 Japanese. They did excellent works with enthusiasm and had fruitful discussions throughout 10 days.
The course provided the study of developmental genetics of Zebrafish and Medaka with the high quality skills by the distinguished lecturer from inside and outside of NIBB. Drs. Shinichi Higashijima (NIPS), Kiyoshi Naruse (NIBB), Kohei Hatta (Univ. Hyogo), Shoji Fukamachi (Univ. Konstanz) were invited as the lecturers.
In addition, excellent researches using zebrafish and medaka were shown by
Drs. Jochen Wittbrodt (Univ. Heidelberg), Kataaki Okubo (NIBB), Masahiko Hibi (RIKEN CDB), Mikiko Tanaka (Tokyo Inst. Tech.), Koichi Kawakami (NIG).
It is a great opportunity to learn high skills and communicate with scientists in the well-prepared facility.
Monday, March 3rd, 2008
10:00-10:30 Registration (Meeting Room at NIBB B1 Floor)
10:30-11:00 Opening Remarks
11:00-13:00 Lecture on Exp. 1 “Bac homologous recombination techniques for zebrafish transgenesis”
13:00-14:00 Lunch
14:00-17:30 Exp. 1 (PCR for targeting DNA. Purification and preparation of the targeting DNA)
17:30-19:00 Special Seminar (by Dr. Wittbrodt)
19:00-20:30 Welcome Party at the Staffs’ Hall
Tuesday, March 4th, 2008
09:00-11:00 Exp.1 (Demonstration of injection by Koshida & Higashijima)
11:00-12:00 Exp.1 (Agarose gel electrophoresis of the purified DNA)
12:00-13:00 Lunch
13:00-15:30 Exp.1 (Homologous recombination and transformation)
15:30-17:00 Evening Lecture I (by Dr. Okubo (NIBB))
Wednesday, March 5th, 2008
09:00-12:00 Exp.2 (Kaede and Dronpa injection into zebrafish embryos)
12:00-13:00 Lunch
13:00-14:30 Lecture on Exp.2 “Tracing cell lineages with a fluorescent protein Kaede and Dronpa”
14:30-16:00 Exp. 2 (Photoconversion I)
16:00-17:00 Exp.1 (Picking up colonies)
17:00-19:00 Social hour I (Presentation by participants)
Thursday, March 6th, 2008
09:00-12:00 Exp. 1 (PCR of picked clones) & Exp. 2 (Photoconversion II)
12:00-13:00 Lunch and Group Photo
13:00-16:00 Exp. 2 (Observation of injected embryos under microscopes)
16:00-18:00 Lecture on Exp. 3 “How to clone your favorite medaka mutants”
18:00-20:00 Social hour II (Presentation by participants)
Friday, March 7th, 2008
09:00-12:00 Exp. 1 (BAC injection into cytoplasm of zebrafish embryos)
12:00-13:00 Lunch
13:00-17:30 Exp. 3 (PCR with balked samples by Mmarkers, electrophoresis and determination of candidate LG)
17:30-19:00 Evening Lecture II (by Dr. Hibi (RIKEN CDB)
Saturday, March 8th, 2008
Lab is opened for participants.
09:00-17:00 Exp. 1 (Injection & Exp.3 (continued)
Sunday, March 9th, 2008
Lab is closed.
Monday, March 10th, 2008
09:00-12:00 Exp. 1 (Observation of injected embryos under microscopes)
12:00-13:00 Lunch
13:00-17:30 Exp. 3 (Determination of the map position of the mutant locus and the most adjacent DNA markers of the mutant locus)
17:30-19:00 Evening lecture III (by Dr. Tanaka (TITec)
Tuesday, March 11th, 2008
09:00-12:00 Exp. 3 (Identification of responsible region of mutant phenotype on the medaka draft genome)
12:00-13:00 Lunch
13:00-15:00 Exp. 3 (Narrowing down responsible regions with DNA markers and identification of mutation site by DNA sequencing)
15:00-16:30 Seminar I (by Dr. Fukamachi)
16:30-18:00 Exp. 3 (continued)
18:00-20:00 Farewell Party (at Kikyu)
Wednesday, March 12th, 2008
09:00-11:00 Seminar II (by Dr. Kawakami (NIG))
11:30-12:00 Closing Remarks
