National Institute for Basic Biology
| Organizers | Takehiko Kobayashi |
|---|---|
| Venue | National Institute for Basic Biology, Okazaki , 444-8585, Japan |
| Date | Nov. 5-8, 2005 |
| Link | Official Website （http://www.nibb.ac.jp/conf51/） |
| Poster |
|
Gene amplification not only results in genome alteration, it also plays wide-ranging roles in many biological function. In multicellular organisms, it is observed in many stages of the life-cycle, such as development, differentiation, senescence, and tumorgensis. In unicellular organisms, it is one of the main strategies for adaptation to the surroundings. Moreover, it is well-known that gene amplification has played critical roles in evolution.
Despite its involvement in these important biological functions, gene amplification has not been a central focus for discussion. One of the reasons is the mechanisms responsible have remained elusive. In this conference, we called researchers studying DNA recombination, DNA replication, chromatin structure and evolution, and discussed the molecular mechanisms and biological functions of gene amplification (including maintenance mechanism of amplified genes).
In this conference, we had ~50 participants including 11 researchers from foreign countries. There were 22 oral and 11 poster presentations. We had exciting discussion and a lot of exchange of information.
Now, with the enormous increases in genomic information and the rapid progression of molecular biology, we are in a good position to look at new aspects of gene amplification. At this point, this conference was timely and good opportunity to develop amplification study.
| Program | |
| November 5, (Sat) | |
| 17:30-18:15 | Registration |
| 18:15-18:20 | Welcome address -Motoya Katsuki (Director-General, NIBB) - |
| 18:20-18:30 | Introduction-Takehiko Kobayashi - |
| Keynote Presentation | |
| Chair: Takashi Horiuchi | |
| 18:30-19:30 | John Roth (Univ. of California-Davis, USA) |
| "A model for the origin of duplications and amplification of genes under selection" | |
| 19:30-20:30 | - Get Together at Conference Hall - |
| November 6, (Sun) | |
| Session 1 | |
| Chair: Hiroshi Iwasaki | |
| 9:00-9:45 | Benoit Arcangioli (Institute Pasteur, France) |
| "Sister chromatid recombination pathway allowing replication fork restart" | |
| 9:45-10:15 | Takashi Hishida (Osaka Univ., Osaka) |
| "Physical and functional interaction of Mgsl with PCNA: impact on rad6-dependent DNA damage tolerance" | |
| 10:15-10:35 | - Coffee Break- |
| Chair: Benoit Arcangioli | |
| 10:35-11:20 | Hiroshi Iwasaki (Yokohama City Univ., Yokohama) |
| "In vivo evidence that an S. pombe Swi5/Sfrl complex is a novel Rad51 mediator" | |
| 11:20-12:05 | Takehiko Shibata (Riken, Wako) |
| "Interplay of DNA recombination, repair and replication in yeast mitochondria" 12:05-14:00 | |
| 12:05-14:00 | - Lunch and Poster at Conference Hall - |
| Session 2 | |
| Chair: Takehiko Kobayashi | |
| 14:00-14:45 | José Sogo (ETH, Switzerland) |
| "Repair of distinct replication-dependent DNA double-strand breaks at the ribosomal replication fork barrier" | |
| 14:45-15:30 | Pablo Hernández (CSIC, Spain) |
| "Cis- and trans-acting factors involved in replication fork blockage at the repeated rRNA genes of fission yeast" | |
| 15:30-15:50 | - Coffee Break- |
| Chair: José Sogo | |
| 15:50-16:35 | Takehiko Kobayashi (NIBB, Okazaki) |
| "Gene amplification regulated by noncoding transcription." | |
| 16:35-17:20 | Hisaji Maki (NAIST, Nara) |
| "Involvement of rDNA locus in monitoring abnormality in the initiation program of chromosomal DNA replication by DNA damage checkpoint control" | |
| 17:20-17:40 | - Coffee Break- |
| Chair: Takehiko Shibata | |
| 17:40-18:25 | John Tower (Univ. of Southern California, USA) |
| Chromatin regulation of Drosophila chorion gene amplification. | |
| - Dinner at DA/KON-YA (Optional) - | |
| November 7, (Mon) | |
| Session 3 | |
| Chair: Noriaki Shimizu | |
| 9:00-9:45 | Michelle Debatisse (Institute Curie, France) |
| Replication Pattern and Genome Instability in Mammalian Cells. | |
| 9:45-10:30 | Hiroki Sasaki (National Cancer Center, Tokyo) |
| Functional and Structural Characteristics of Amplicons in Human Cancer | |
| 10:30-10:50 | - Coffee Break - |
| Chair: Michelle Debatisse | |
| 10:50-11:35 | Noriaki Shimizu (Hiroshima Univ., Hiroshima) |
| "Gene amplification in mammalian cells-its mechanism and the product's behavior-" | |
| 11:35-12:20 | Aaron Bensimon (Institute Pasteur, France) |
| Human rDNA is a mosaic of canonical and palindromic units | |
| 12:20-13:30 | - Lunch and Poster at Conference Hall - |
| Session 4 | |
| Chair: Maki Hisaji | |
| 13:30-14:15 | David Shore (Univ. of Geneva, Switzerland) |
| "A feedback loop controling rDNA copy number and Sir2 protein levels in yeast" | |
| 14:15-15:00 | Katsuki Johzuka (NIBB, Okazaki) |
| "Maintenance of the rDNA repeats by the FOB1 and condensin complex" | |
| 15:00-15:20 | - Coffee Break- |
| Chair: David Shore | |
| 15:20-16:05 | Alexander Strunnikov (NIH, USA) |
| "Recognition of repeated and unique chromosomal binding sites by condensin: genetic and epigenetic controls" | |
| 16:05-16:50 | Jorge Schvartzman (CSIC, Spain) |
| "Identification of Different Forms of Replication Fork Blockage and its Consequences" | |
| 16:50-17:10 | - Coffee Break - |
| Chair: Jorge Schvartzman | |
| 17:10-17:55 | Akihiko Kikuchi (Nagoya Univ., Nagoya) |
| "Topoisomerases are obligatory for Mitotic Chromosomes?" | |
| -Reception at Okazaki New Grand Hotel- | |
| November 8, (Tue) | |
| Session 5 | |
| Chair: Kirill Lobachev | |
| 9:00-9:45 | Austen Ganley (NIBB, Okazaki) |
| "The role of amplification in concerted evolution: A new model from experimental evolution data in yeast" | |
| 9:45-10:05 | Takaaki Watanabe (NIBB, Okazaki) |
| "A novel gene amplification system in yeast" | |
| 10:05-10:25 | - Coffee Break - |
| Chair: Akihiko Kikuchi | |
| 10:25-11:10 | Kirill Lobachev (Georgia Institute of Technology, USA) |
| "Double minutes, homogeneously staining regions and unstable mosaic chromosomes result from hairpin-capped double-strand breaks" | |
| 11:10-11:30 | Takashi Horiuchi (NIBB, Okazaki) |
| "E. coli cell with a linear genome" | |
| 11:30-11:40 | Closing Remarks - David Shore - |
| 11:40-13:00 | - Lunch and Poster at Conference Hall - |
| Departure | |
