Dr. Chun-che Chang （張俊哲） National Taiwan University

2009年07月09日(木) 16:00 より 17:00 まで

山手地区３号館９階セミナー室B

発生遺伝学部門 重信 秀治 内線5875 shige@nibb.ac.jp

Chang博士は、”evo-devo”の視点から、昆虫の生殖細胞発生を遺伝子レベルで研究されています。今回は、バッタやアブラムシなどのvasa・nanos遺伝子の研究を中心としたお話を伺えると思います。 In animals germ cells are specified during embryogenesis; this specification relies either on a maternally inherited germ plasm in early development or induction signals from neighboring somatic cells in mid or late development. Whether the modes of germline specification are associated with embryonic pattern formation or with phylogenetic relationship is still not clear. Nevertheless, it has been demonstrated that many animals utilize two highly conserved germline genes, vasa and nanos, for germline development regardless which specification mode they employ. In order to examine the diversity of germline specification in insects, we produced a highly cross-reacting antibody formosa (for most Vasa) that recognize conserved Vasa-specific motifs in many species. In this presentation I shall describe how we take advantage of such an antibody to explore germline specification in the grasshopper Schistocerca gregaria and the pea aphid Acyrthosiphon pisum, two hemimetabolous insects whose body plan is determined differently from the model holometabolous insect Drosophila melanogaster. In addition, I will present our recent findings concerning the establishment of egg polarity and the migration of germ cells in the pea aphid. Taken together, these data allow us to shed new light on germline development in insects. References Ting-Yu Huang, Charles E. Cook, Gregory K. Davis, Shuji Shigenobu, Rita P.-Y. Chen, and Chun-che Chang (2009) Anterior development in the parthenogenetic and viviparous form of the pea aphid, Acyrthosiphon pisum: hunchback and orthodenticle expression. Insect Mol. Biol. (submitted) Chun-che Chang, Ting-Yu Huang, Charles E. Cook, Gee-Way Lin, Chun-Liang Shih, and Rita P.-Y. Chen (2009) Developmental expression of Apnanos during oogenesis and embryogenesis in the parthenogenetic pea aphid Acyrthosiphon pisum (Hemiptera: Aphidoidea). Int. J. Dev. Biol. 53: 169-176. Taro Mito, Taro Nakamura, Isao Sarashina, Chun-che Chang, Shotaro Ogawa, Hideyo Ohuchi, and Sumihare Noji (2008) Dynamic expression patterns of vasa during embryogenesis in the cricket Gryllus bimaculatus. Dev. Genes Evol. 218: 381-387. Chun-che Chang, Ting-Yu Huang, Chun-Liang Shih, Gee-Way Lin, Te-Pin Chang, Hui Chiu, Wen-Chang Chang (2008) Whole-mount identification of gene transcripts in aphids: Protocols and evaluation of probe accessibility. Arch. Insect Biochem. Physiol. 68: 186-196. Chun-che Chang, Hui Chiu, and How-Jing Lee (2008) Identification of nuage-like structures in the panoistic ovarioles of the German cockroach Blattella germanica (Linnaeus). Formosan Entomologist 28: 1-10. (cover article) Chun-che Chang, Gee-way Lin, Charles E. Cook, Shwu-bin Horng, How-jin Lee and Ting-yu Huang (2007) Apvasa marks germ-cell migration in the parthenogenetic pea aphid Acyrthosiphon pisum (Hemiptera: Aphidoidea). Dev. Genes Evol. 217: 275-287. Chun-che Chang, Wen-chih Lee, Charles E. Cook, Gee-way Lin, and Tschining Chang (2006) Germ-plasm specification and germline development in the parthenogenetic pea aphid Acyrthosiphon pisum: Vasa and Nanos as markers. Int. J. Dev. Biol. 50: 413-421. Chun-che Chang, Peter Dearden, and Michael Akam (2002) Germline development in the grasshopper Schistocerca gregaria: Vasa as a marker. Dev. Biol. 252, 110-118. (cover article)