大学共同利用機関法人 自然科学研究機構



国際共同研究成果 - 成果報告

Evidence for multiple, developmentally regulated isoforms of Ptprq on hair cells of the inner ear.

Authors Nayak G, Goodyear RJ, Legan PK, Noda M, Richardson GP.
Journal Dev Neurobiol. 2011 Feb;71(2):129-41.
Link PudMed (http://www.ncbi.nlm.nih.gov/pubmed/20715155)

基礎生物学研究所統合神経生物学研究部門とUniversity of Sussex, UK との共同研究成果です。


Ptprq is a receptor-like inositol lipid phosphatase associated with the shaft connectors of hair bundles. Three lines of evidence suggest Ptprq is a chondroitin sulfate proteoglycan: (1) chondroitinase ABC treatment causes a loss of the ruthenium-red reactive, electron-dense particles associated with shaft connectors, (2) chondroitinase ABC causes an increase in the electrophoretic mobility of Ptprq, and (3) hair bundles in the developing inner ear of wild-type mice, but not those of Ptprq(-/-) mice, react with monoclonal antibody (mAb) 473-HD, an IgM that recognizes the dermatan-sulfate-dependent epitope DSD1. Two lines of evidence indicate that there may be multiple isoforms of Ptprq expressed in hair bundles. First, although Ptprq is expressed throughout the lifetime of most hair cells, hair bundles in the mouse and chick inner ear only express the DSD1 epitope transiently during development. Second, mAb H10, a novel mAb that recognizes an epitope common to several avian inner-ear proteins including Ptprq, only stains mature hair bundles in the extrastriolar regions of the vestibular maculae. MAb H10 does not stain mature hair bundles in the striolar regions of the maculae or in the basilar papilla, nor does it stain immature hair bundles in any organ. Three distinct, developmentally regulated isoforms of Ptprq may therefore be expressed on hair bundles of the chick inner ear. Hair bundles in the mature chick ear that do not express the H10 epitope have longer shaft connectors than those that do, indicating the presence or absence of the H10 epitope on Ptprq may modulate the spacing of stereocilia.