大学共同利用機関法人 自然科学研究機構



国際共同研究成果 - 成果報告

Functional hierarchy and reversibility within the murine spermatogenic stem cell compartment.

Authors Nakagawa T, Sharma M, Nabeshima Y, Braun RE, Yoshida S.
Journal Science. 2010 Apr 2;328(5974):62-7.
Link PubMed (http://www.ncbi.nlm.nih.gov/pubmed/20299552)

基礎生物学研究所生殖細胞研究部門とThe Jackson Laboratory, USAなどとの共同研究成果です。


Stem cells support tissue maintenance by balancing self-renewal and differentiation. In mice, it is believed that a homogeneous stem cell population of single spermatogonia supports spermatogenesis, and that differentiation, which is accompanied by the formation of connected cells (cysts) of increasing length, is linear and nonreversible. We evaluated this model with the use of lineage analysis and live imaging, and found that this putative stem cell population is not homogeneous. Instead, the stem cell pool that supports steady-state spermatogenesis is contained within a subpopulation of single spermatogonia. We also found that cysts are not committed to differentiation and appear to recover stem cell potential by fragmentation, and that the fate of individual spermatogonial populations was markedly altered during regeneration after damage. Thus, there are multiple and reversible paths from stem cells to differentiation, and these may also occur in other systems.