National Institute for Basic Biology
Organizers | Kiyoshi NARUSE (NIBB and NBRP Medaka) Laszlo ORBAN (TLL) Yasuhiro KAMEI (NIBB) Hitoshi OKAMOTO (RIKEN and NBRP Zebrafish) Shinji TAKADA (NIBB) Shin-ichi HIGASHIJIMA (NIBB) Kiyoshi TATEMATSU (NIBB) Yusuke TAKEHANA (NIBB) |
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Venue | National Institute for Basic Biology (NIBB), Okazaki, Japan |
Date | Aug. 18-30, 2016 |
Link | Web site (http://www.nibb.ac.jp/course9/) |
Poster | |
The 9th NIBB International Practical Course - 4th NIBB-TLL Joint International Practical Course was held from August 18 to 30, 2016. We received 28 applications, and 17 (8 males, 9 females) passed the screening process. The participants comprised a group of 7 from Japan and 10 from overseas of 10 regions in 7 countries. Dr. Laszlo Orban, one of the organizers of the program, attended as a special lecturer as well, and we invited other researchers to give special lectures during the course such as Dr. Zoltan Varga, who is the director of the Zebrafish International Resource Center, the University of Oregon, Dr. Rie Goto from Ehime University, and Dr. Goro YOSHIZAKI from Tokyo University of Marine Science and Technology. Moreover we invited 7 lecturers from both Japan and abroad to give seminars. The course was covered the topics “Highly efficient transgenesis using the PhiC31 system”, “Gene knock-in using the CRISPR/Cas9 system in zebrafish as well as medaka”, “Cryopreservation of sperm and artificial insemination in medaka”, “Imaging using light-sheet microscopes”, and “Gene expression induction method by IR-LEGO”.
We managed the course without supporting the travel costs for students, however in spite of having supported travel costs in the past we had just under twice as many applicants for this year’s course. It was the first time we hosted participants from South America, of which we had two. We were also pleased that we received students from both EMBL and TLL, two of NIBB’s research cooperation institutes.
We plan to continue to conduct NIBB International Practical Courses which feature small fish research with a combination of basic laboratory techniques and the latest technology in the future.
Kiyoshi Natuse on behalf of the organizing committee
Thu, August 18th, 2016 | |
14:00 | Registration |
16:00 - 17:00 | Opening Remarks & Orientation |
17:00 - 18:00 | Seminar by Dr. Laszlo ORBAN (TLL) |
18:00 - 20:00 | Welcome Party (Opening discussion on medaka and zebrafish) |
Fri, August 19th, 2016 | |
9:00 - 12:00 | CRISPR lecture by Dr. ANSAI |
RNA synthesis | |
13:00 - 17:00 | CRISPR lecture by Dr. ANSAI |
RNA precipitation | |
17:00 - 18:00 | Seminar by Dr. Goro YOSHIZAKI (TUMSAT) |
Sat, August 20th, 2016 | |
The 22th Japanese medaka and zebrafish meeting at OCC (Optional) | |
Sun, August 21st, 2016 | |
The 22th Japanese medaka and zebrafish meeting at OCC (Optional) | |
Mon, August 22nd, 2016 | |
9:00 - 12:30 | Microinjection (medaka) |
14:00 - 15:30 | PhiC31 lecture by Dr. ANSAI |
15:30 - 16:30 | Visit to medaka fish facility |
16:30 - 17:30 | Seminar by Dr. Rie Goto (Ehime Univ.) |
17:30 - 19:30 | Social Hour (Presentation of participants and discussion with lecturers) |
Tue, August 23rd, 2016 | |
9:00 - 12:00 | Microinjection (medaka) |
13:00 - 17:00 | Knock-in lecture by Dr. HIGASHIJIMA |
Genome database practical by Dr. NARUSE | |
17:00 - 18:00 | Seminar by Dr. Zoltán VARGA (ZIRC) |
18:00 - 20:00 | Social Hour (Presentation of participants and discussion with lecturers) |
Wed, August 24th, 2016 | |
9:00 - 12:00 | Microinjection (zebrafish) |
13:00 - 17:00 | Dissection of zebrafish retina |
Imaging | |
17:00 - 18:00 | Seminar by Dr. Sachihiro SUZUKI (OIST) |
18:00 - 18:30 | Seminar by Dr. Masayuki HATTORI (NIBB) |
Thu, August 25th, 2016 | |
9:00 - 12:00 | Cryopresavation of medaka sperm |
13:00 - 17:00 | Artificial insemination |
17:00 - 18:00 | Seminar by Dr. Tomonori DEGUCHI (AIST) |
18:00 - 18:30 | Seminar by Dr. Saori YOKOI (NIBB) |
Fri, August 26th, 2016 | |
9:00 - 12:00 | IR-LEGO |
Behavior assay | |
13:00 - 17:00 | IR-LEGO |
Behavior assay | |
17:00 - 18:00 | Seminar by Dr. Shinya KOMOTO (EMBL) |
Sat, August 27th, 2016 | |
No schedule | |
Sun, August 28th, 2016 | |
No schedule | |
Mon, August 29th, 2016 | |
9:00 - 10:30 | Seminar by Dr. Shigenori NONAKA (NIBB) |
Imaging lecture by Dr. Nonaka | |
10:30 - 11:00 | Seminar by Dr. Akiko Kondow (Fujita Health Univ.) |
12:00 - 15:00 | Group 1: DSLM/Group 2: CLSM |
15:00 - 18:00 | Group 1: CLSM/Group 2: DSLM |
Tue, August 30th, 2016 | |
9:00 - 12:00 | Phenotype observation |
DNA isolation | |
13:00 - 18:00 | PCR/HMA genotyping |
Behavior analysis by Dr. Yokoi | |
18:00 |
Farewell Party (Group discussion for future research on small fish and feedback
on the course)
|
Wed, August 31st, 2016 | |
Departure |