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The 9th NIBB International Practical Course The 4th NIBB-TLL Joint International Practical Course "Genetics and Imaging of Medaka and Zebrafish"

Organizers Kiyoshi NARUSE (NIBB and NBRP Medaka)
Laszlo ORBAN (TLL)
Yasuhiro KAMEI (NIBB)
Hitoshi OKAMOTO (RIKEN and NBRP Zebrafish)
Shinji TAKADA (NIBB)
Shin-ichi HIGASHIJIMA (NIBB)
Kiyoshi TATEMATSU (NIBB)
Yusuke TAKEHANA (NIBB)
Venue National Institute for Basic Biology (NIBB), Okazaki, Japan
Date Aug. 18-30, 2016
Link Web site (http://www.nibb.ac.jp/course9/)
Poster

practical9_group.jpg
 

The 9th NIBB International Practical Course - 4th NIBB-TLL Joint International Practical Course was held from August 18 to 30, 2016. We received 28 applications, and 17 (8 males, 9 females) passed the screening process. The participants comprised a group of 7 from Japan and 10 from overseas of 10 regions in 7 countries. Dr. Laszlo Orban, one of the organizers of the program, attended as a special lecturer as well, and we invited other researchers to give special lectures during the course such as Dr. Zoltan Varga, who is the director of the Zebrafish International Resource Center, the University of Oregon, Dr. Rie Goto from Ehime University, and Dr. Goro YOSHIZAKI from Tokyo University of Marine Science and Technology. Moreover we invited 7 lecturers from both Japan and abroad to give seminars. The course was covered the topics “Highly efficient transgenesis using the PhiC31 system”, “Gene knock-in using the CRISPR/Cas9 system in zebrafish as well as medaka”, “Cryopreservation of sperm and artificial insemination in medaka”, “Imaging using light-sheet microscopes”, and “Gene expression induction method by IR-LEGO”.

We managed the course without supporting the travel costs for students, however in spite of having supported travel costs in the past we had just under twice as many applicants for this year’s course. It was the first time we hosted participants from South America, of which we had two. We were also pleased that we received students from both EMBL and TLL, two of NIBB’s research cooperation institutes.

We plan to continue to conduct NIBB International Practical Courses which feature small fish research with a combination of basic laboratory techniques and the latest technology in the future.

 

Kiyoshi Natuse on behalf of the organizing committee

Program
Thu, August 18th, 2016  
14:00 Registration
16:00 - 17:00 Opening Remarks & Orientation
17:00 - 18:00 Seminar by Dr. Laszlo ORBAN (TLL)
18:00 - 20:00 Welcome Party (Opening discussion on medaka and zebrafish)
   
Fri, August 19th, 2016  
9:00 - 12:00 CRISPR lecture by Dr. ANSAI
  RNA synthesis
13:00 - 17:00 CRISPR lecture by Dr. ANSAI
  RNA precipitation
17:00 - 18:00 Seminar by Dr. Goro YOSHIZAKI (TUMSAT)
   
Sat, August 20th, 2016  
The 22th Japanese medaka and zebrafish meeting at OCC (Optional)
   
Sun, August 21st, 2016  
The 22th Japanese medaka and zebrafish meeting at OCC (Optional)
   
Mon, August 22nd, 2016  
9:00 - 12:30 Microinjection (medaka)
14:00 - 15:30 PhiC31 lecture by Dr. ANSAI
15:30 - 16:30 Visit to medaka fish facility
16:30 - 17:30 Seminar by  Dr. Rie Goto (Ehime Univ.)
17:30 - 19:30 Social Hour (Presentation of participants and discussion with lecturers)
   
Tue, August 23rd, 2016  
9:00 - 12:00 Microinjection (medaka)
13:00 - 17:00 Knock-in lecture by Dr. HIGASHIJIMA
  Genome database practical by Dr. NARUSE
17:00 - 18:00 Seminar by Dr. Zoltán VARGA (ZIRC)
18:00 - 20:00 Social Hour (Presentation of participants and discussion with lecturers)
   
Wed, August 24th, 2016  
9:00 - 12:00 Microinjection (zebrafish)
13:00 - 17:00 Dissection of zebrafish retina
  Imaging
17:00 - 18:00 Seminar by Dr. Sachihiro SUZUKI (OIST)
18:00 - 18:30 Seminar by Dr. Masayuki HATTORI (NIBB)
   
Thu, August 25th, 2016  
9:00 - 12:00 Cryopresavation of medaka sperm
13:00 - 17:00 Artificial insemination
17:00 - 18:00 Seminar by Dr. Tomonori DEGUCHI (AIST)
18:00 - 18:30 Seminar by Dr. Saori YOKOI (NIBB)
   
Fri, August 26th, 2016  
9:00 - 12:00 IR-LEGO
  Behavior assay
13:00 - 17:00 IR-LEGO
  Behavior assay
17:00 - 18:00 Seminar by Dr. Shinya KOMOTO (EMBL)
   
Sat, August 27th, 2016  
  No schedule
Sun, August 28th, 2016  
  No schedule
   
Mon, August 29th, 2016  
9:00 - 10:30 Seminar by Dr. Shigenori NONAKA (NIBB)
  Imaging lecture by Dr. Nonaka
10:30 - 11:00 Seminar by Dr. Akiko Kondow (Fujita Health Univ.)
12:00 - 15:00 Group 1: DSLM/Group 2: CLSM
15:00 - 18:00 Group 1: CLSM/Group 2: DSLM
   
Tue, August 30th, 2016  
9:00 - 12:00 Phenotype observation
  DNA isolation
13:00 - 18:00 PCR/HMA genotyping
  Behavior analysis by Dr. Yokoi
18:00 Farewell Party (Group discussion for future research on small fish and feedback 
on the course)
   
Wed, August 31st, 2016  
Departure