Dr. Lazaro CENTANIN (Center for Organismal Studies, Heidelberg Universität, GERMANY)

2018. 09. 27 (Thu) 17:00 ~ 18:00

Conf. Room, Myodaiji (111)

Spectography and Bioimaging Facility, Yasuhiro Kamei(7535)

The 10^th NIBB International Practical Course
Open Seminar

My lecture will review technical advances on how to perform lineage analysis in vertebrates in general and fish in particular. We will briefly go through classical approaches on how researchers have experimentally approached the issue of establishing lineage relation on embryos that simultaneously go through cell division and differentiation. I will review different ways of labelling and following cells in different animal models, from mechanical manipulation of the specimens (experimental embryology) to modern, interference-free genetic tools. We will then revise the advances on molecular tools such as the Cre/LoxP system and its variations, Brainbow (mice) Zebrabow and Gaudí (zebrafish and medaka respectively). These consist of a series of Fluorescent
Proteins of different colours, which are expressed in a mutually exclusive manner and are inherited from mother to daughter cell. This way, cells that share a given colour (a molecular fingerprint) would have originated from a common progenitor or stem cell. We will see examples in which these methods were used to solve long-standing questions, and we will finish with a perspective on where the field is heading, highlighting interfaces with other techniques that the students will learn about during the NIBB course.