Dr. Yasuko Kamisugi (Centre for Plant Sciences, University of Leeds, UK.)

2017. 01. 11 (Wed) 15:00 ~ 16:00

Conf. Room, Myodaiji (111)

Lab. Evol. Biol. Mitsuyasu Hasebe（7546）

  Uniquely among model plants, the moss Physcomitrella patens preferentially integrates transgenes at homologous sites in the genome, enabling highly efficient targeted mutagenesis for the analysis of gene function. Predicting that the outcome of transgene integration depends on the recruitment of transforming DNA by endogenous DNA-double-strand-break (DNA-DSB) repair, we are investigating the roles of regulators of DNA repair pathway choice. In eukaryotes, the principal DNA-DSB repair pathways are Non-Homologous End Joining (NHEJ) and Homology-dependent Repair (HR). Key triggers of these pathways are the protein kinases ATM (Ataxia Telangiectasia Mutated), required for the execution of the NHEJ pathway, and ATR (ATM and Rad3-related) that regulates HR. In this talk I will describe how we have used targeted mutagenesis of the P. patens ATM, ATR and associated genes to investigate the roles of these kinases in DNA repair, and to determine their relation to the efficiency of transgene integration and gene targeting.