Dr. Paul Matsudaira (Singapore-MIT Alliance for Research and Technology, National University of Singapore)

2017. 02. 21 (Tue) 15:30 ~ 17:00

Conf. Room, Myodaiji (111)

Naoto Ueno (7570), Shoji Mano (7504)

During convergence and extension (CE) at mid-gastrulation stage of the zebrafish embryo, cells collectively move to form the embryonic axis. The biochemical cues and the cell motility of CE have been intensively studied but a remaining challenge is to characterize the local and global mechanics. We combine in-toto imaging by light-sheet microscopy with novel computational methods to calculate the strain map of tissue deformation, during four hours of mid-gastrulation from 7.5hpf to 11.5 hpf. Strain is derived from determining the polar coordinates of all nuclei, extracting the nuclear velocities from fields of nuclei, and extract anterior-posterior, medial-lateral, and radial strain from the change in cell domains. The map identifies areas of linear and rotational strain that correspond to convergent-extension and somatogenesis and detects the onset of strain before morphological changes are apparent.