Analysis of Hydra PIWI proteins and piRNAs uncover early evolutionary origins of the piRNA pathway. R. SM Lim, A. Anand, C. Nishimiya-Fujisawa, S. Kobayashi and T. Kai Dev. Biol., 386, 237-251 (2014)
Enrichment of spermatogonial stem cells using side population in teleost. M. Hayashi, M. Sato, Y. Iwasaki, T. Onozawa, N. Katayama, Y. Nagasaka, S. Sadaie, S. Kobayashi and G. Yoshizaki Biol. Reprod., 91, 23 (2014)
Analysis of a novel gene, Sdgc, reveals sex chromosome–dependent differences of medaka germ cells prior to gonad formation. T. Nishimura, A. Herpin, T. Kimura, I. Hara, T. Kawasaki, S. Nakamura, Y. Yamamoto, T. Saito, J. Yoshimura, S. Morishita, T. Tsukahara, S. Kobayashi, K. Naruse, S. Shigenobu, N. Sakai, M. Schartl and M. Tanaka Development, 141, 3363-3369 (2014)
Pri peptides are mediators of ecdysone for the temporal control of development. H. Chanut-Delalande, Y. Hashimoto, A. Pélissier-Monier, R. Spokony, A. Dib, T. Kondo, J. Bohère, K. Niimi, Y. Latapie, S. Inagaki, L. Dubois, P. Valenti, C. Polesello, S. Kobayashi, B. Moussian, K. White, S. Plaza, Y. Kageyama and F. Payre Nat. Cell Biol., 16, 1035-1044 (2014)
H3K36 trimethylation-mediated epigenetic regulation is activated by Bam and promotes germ cell differentiation during early oogenesis in Drosophila. M. Mukai, S. Hira, K. Nakamura, S. Nakamura, H. Kimura, M. Sato and S. Kobayashi Biol Open, 4, 119-124 (2014)
Autocrine regulation of ecdysone synthesis by b3-octopamine receptor in the prothoracic gland is essential for Drosophila metamorphosis. Y. Ohhara, Y. Shimada-Niwa, R. Niwa, Y. Kayashima, Y. Hayashi, K. Akagi, H. Ueda, K. Yamakawa-Kobayashi and S. Kobayashi Proc. Natl. Acad. Sci. U.S.A., 112, 1452-1457 (2015) >> 詳細 NIBB ニュース
Hierarchical differentiation competence in response to retinoic acid ensures stem cell maintenance during mouse spermatogenesis. K. Ikami, M. Tokue, R. Sugimoto, C. Noda, S. Kobayashi, K. Hara and S. Yoshida Development, 142, 1582-1592 (2015)
Sex determination.foxl3 is a germ cell-intrinsic factor involved in sperm-egg fate decision in medaka. T. Nishimura, T. Sato, Y. Yamamoto, I. Watakabe, Y. Ohkawa, M. Suyama, S. Kobayashi and M. Tanaka Science, 349, 328-331. (2015)
Conserved role of Ovo in germline development in mouse and Drosophila. M. Hayashi, Y. Shinozuka, S. Shigenobu, M. Sato, M. Sugimoto, S. Ito, K. Abe
and S. Kobayashi Sci. Rep., 6, 40056 (2017) >> 論文 NIBBニュース
Nutrient-Dependent Endocycling in Steroidogenic Tissue Dictates Timing of Metamorphosis in Drosophila melanogaster. Y. Ohhara, S. Kobayashi and N. Yamanaka PLOS Genetics, 13-1, e1006583 (2017)
SHISA6 confers resistance to differentiation-promoting Wnt/β-catenin signaling in mouse spermatogenic stem cells. M. Tokue, K. Ikami, S. Mizuno, C. Takagi, A. Miyagi, R. Takada, C. Noda, Y. Kitadate, K. Hara, H. Mizuguchi, T. Sato, M. M. Taketo, F. Sugiyama, T. Ogawa, S. Kobayashi, N. Ueno, S. Takahashi, S. Takada and S. Yoshida Stem Cell Reports, 8, 561-575 (2017)
Transcriptome profiling of the spermatheca identifies genes potentially involved in the long-term sperm storage of ant queens. A. Gotoh, S. Shigenobu, K. Yamaguchi, S. Kobayashi, F. Ito and K. Tsuji Sci. Rep., 7: 40056 (2017)
The transcripts immunoprecipitated with Sxl protein in the primordial germ cells of Drosophila embryos. : R. Ota, S. Morita, M. Sato, S. Shigenobu, M. Hayashi, and S. Kobayashi Dev. Growth. Differ., 59, 713-723 (2017)
Transcriptome characterization of male accessory glands in ants to identify molecules involved in their reproductive success. A. Gotoh, S. Shigenobu, K. Yamaguchi, S. Kobayashi, F. Ito and K. Tsuji
Insect Molecular Biology, DOI 10.1111/imb.12364 (2017)
Maternal Nanos-dependent RNA stabilization in the primordial germ cells of Drosophila embryos. S. Sugimori, Y. Kumata, and S. Kobayashi Dev. Growth. Differ., 60, 63-75 (2017)
Adult-specific insulin-producing neurons in Drosophila melanogaster. Y. Ohhara, S. Kobayashi, K. Yamakawa-Kobayashi, N. Yamanaka Journal of Comparative Neurology, (in press) (2018)
High-resolution DNA methylome analysis of primordial germ cells identifies gender-specific reprogramming in mice. Kobayashi, H., Sakurai, T., Miura, F., Imai, M., Mochiduki, K., Yanagisawa, E., Sakashita, A., Wakai, T., Suzuki, Y., Ito, T., Matsui ,Y., Kono, T*. Genome Research, 23, 616-627 (2013)
Max is a repressor of germ-cell-related gene expression in mouse embryonic stem cells. Maeda, I., Okamura, D., Tokitake, Y., Ikeda, M, Kawaguchi, H., Mise, N., Abe, K., Noce, T., Okuda, A, Matsui, Y*. Nature Communications, 4, 1754 (2013)
On the fate of primordial germ cells injected into early mouse embryos. Leitch, H.G*., Okamura, D., Durcova-Hills, G., Stewart, C.L., Gardner, R.L., Matsui, Y., Papaioannou, V.E Developmental Biology, 385, 155-159 (2013)
The majority of early primordial germ cells acquire pluripotency by AKT activation. Matsui, Y*., Takehara, A., Tokitake, Y., Ikeda, M., Obara, Y., Morita-Fujimura, Y., Kimura, T., and Nakano, T. Development, 141, 4457-4467 (2014)
>> 東北大学 大学院 生命科学研究科 研究成果
Sall4 is essential for mouse primordial germ cell specification by suppressing somatic cell program genes. Yamaguchi, Y. L., Satomi S. Tanaka, S. S., Kumagai, M, Yuka Fujimoto, Y., Terabayashi, T., Matsui, Y. and Nishinakamura, R*. Stem Cells, 33, 289-300 (2015)
Novel sex-dependent differentially methylated regions are demethylated in adult male mouse livers. Ito, S., Hirabayashi, K., Moriishi, K. Matsui, Y., Moriya, K., Koike, K., Matsuura, Y., Shiota, K. and Yagi, S*. Biochemical and Biophysical Research Communications, 462, 332-338 (2015)
Sex Specification and Heterogeneity of Primordial Germ Cells in Mice. Sakashita, A., Kawabata, Y., Jincho, Y., Tajima, S., Kumamoto, S., Kobayashi, H., Matsui, Y. and Kono, T*. PLoS ONE, 10, e0144836 (2015)
Loss of MAX results in meiotic entry in mouse embryonic and germline stem cells. Suzuki, A., Hirasaki, M., Hishida, T., Wu, J., Okamura, D., Ueda, A., Nishimoto, M., Nakachi, Y., Mizuno, Y., Okazaki, Y., Matsui, Y., Izpisua Belmonte, J.C., Okuda, A*. Nature Communications, 7, 11056 (2016)
Transgenic Expression of Telomerase reverse transcriptase (Tert) Improves Cell Proliferation of Primary Cells and Enhances Reprogramming Efficiency into the Induced Pluripotent Stem Cell. Hidema, S., Fukuda, T., Date, S., Tokitake, Y., Matsui, Y., Nishimori, K*., Sasaki, H. Bioscience, Biotechnology, and Biochemistry, 80, 1925-1933 (2016)
Selective de-repression of germ cell-specific genes in mouse embryonic fibroblasts in a permissive epigenetic environment. Sekinaka, T., Hayashi, Y., Noce, T., Niwa, H., Matsui, Y*. Scientific Reports, 6:32932 (2016)
DNA Methylation of the Fthl17 5’-Upstream Region Regulates Differential Fthl17 Expression in Lung Cancer Cells and Germline Stem Cells. Aoki, N., Mochizuki, K., Matsui, Y*. PLoS ONE, 12, e0172219 (2017)
Distinct requirements for energy metabolism in mouse primordial germ cells and their reprogramming to embryonic germ cells. Hayashi, Y., Otsuka, K., Ebina, M., Igarashi, K., Takehara, A., Matsumoto, M., Kanai, A., Igarashi, K., Soga, T., Matsui, Y*. Proceedings of National Academy of Science, USA. 114, 8286-8294 (2017)
>> 東北大学 大学院 生命科学研究科 研究成果
Dnd1-mediated epigenetic control of teratoma formation in mouse. Gu, W., Mochizuki, K., Otsuka, K., Hamada, R., Takehara, A., Matsui, Y*. Biology Open, 7, bio030106 (2018)
Differentiation of zebrafish spermatogonial stem cells to functional sperm in culture. Kawasaki, T., Siegfried, K. R., Sakai, N.* Development, 143, 566-574 (2016)
Allogeneic transplantation of testicular hyperplasia in rag1 mutant zebrafish. Kawasaki, T., Sakai, N.*Bio-protocol 6, e1992 (2016)
Development and growth of organs in living whole embryo and larval grafts in zebrafish. Kawasaki, T., Maeno, A., Shiroishi, T., Sakai, N.* Scientific Reports, 7, 16508 (2017)
Differentiation of zebrafish spermatogonial stem cells to functional sperm in culture. Kawasaki, T., Siegfried, K. R., Sakai, N.* Development, 143, 566-574 (2016)
Allogeneic transplantation of testicular hyperplasia in rag1 mutant zebrafish. Kawasaki, T., Sakai, N.* Bio-protocol 6, e1992 (2016)
Development and growth of organs in living whole embryo and larval grafts in zebrafish. Kawasaki, T., Maeno, A., Shiroishi, T., Sakai, N.* Scientific Reports, 7, 16508 (2017)
MAFB is dispensable for the fetal testis morphogenesis and the maintenance of spermatogenesis in adult mice.: HH Shawki*, H Oishi*, T Usui, Y Kitadate, WA Basha, AM Abdellatif, K Hasegawa, R Okada, K Mochida, HA El-Shemy, M Muratani, A Ogura, S Yoshida, S Takahashi PLoS ONE, 13(1): e019080
Tokue, M., Ikami, K., Mizuno, S., Takagi, C., Miyagi, A., Takada, R., Noda, C., Kitadate, Y., Hara, K., Mizuguchi, H., Sato, T., Taketo, M. M., Sugiyama, F., Ogawa, T., Kobayashi, S., Ueno, N., Takahashi,, S., Takada, S., and *Yoshida, S. SHISA6 confers resistance to differentiation-promoting Wnt/β-catenin signaling in mouse spermatogenic stem cells. Stem Cell Report,s 8: 561-575, 2017 >> NIBB ニュース
Ikami, K., Tokue, M., Sugimoto, R., Noda, C., Kobayashi, S., Hara, K., and *Yoshida, S. Hierarchical differentiation competence in response to retinoic acid ensures stem cell maintenance during mouse spermatogenesis. Development, 142:1582-1592, 2015 >> NIBB ニュース
K. Ikegami, Y. Atsumi, E. Yorinaga, H. Ono, I. Murayama, Y. Nakane, W. Ota, N. Arai, A. Tega, M. Iigo, V. M. Darras, K. Tsutsui, Y. Hayashi, S. Yoshida, and *T. Yoshimura: Low temperature-induced circulating triiodothyronine accelerates seasonal testicular regression. Endocrinology Published Online November 18, 2014
K. Hara, T. Nakagawa, H. Enomoto, M. Suzuki, M. Yamamoto, *B. D. Simons and *S. Yoshida: Mouse spermatogenic stem cells continually interconvert between equipotent singly isolated and syncytial states. Cell Stem Cell 14:658-672, 2014 >> NIBB ニュース
Shirakawa T, Yaman-Deveci R, Tomizawa S, Kamizato Y, Nakajima K, Sone H, Sato Y, Sharif J, Yamashita A, Takada-Horisawa Y, Yoshida S, Ura K, Muto M, Koseki H, Suda T and Ohbo K. An epigenetic switch is crucial for spermatogonia to exit the undifferentiated state toward a Kit-positive identity. Development, 140 3565-3576, 2013 >> 詳細
Uemura M, Ozawa A, Nagata T, Kurasawa K, Tsunekawa N, Nobuhisa I, Taga T, Hara K, Kudo A, Kawakami H, Saijoh Y, Kurohmaru M, Kanai-Azuma M and Kanai Y. Sox17 haploinsufficiency results in perinatal biliary atresia and hepatitis in C57BL/6 background mice. Development, 140: 639-648, 2013
An epigenetic switch is crucial for spermatogonia to exit the undifferentiated state toward a Kit-positive identity. Shirakawa T., Yaman-Deveci R., Tomizawa S., Kamizato Y., Nakajima K., Sone H., Sato Y., Sharif J., Takada-Horisawa Y., Yoshida S., Ura K., Muto M., Koseki K., Suda T. and ＊Ohbo K. Development, 140:3565-3576, 2013 >> 詳細
The RNA binding protein Nanos2 organizes a post-transcriptional buffering system to retain primitive state of mouse spermatogonial stem cells. Zhou, Z., Shirakawa, T., Ohbo, K., Sada, A., Wu, Q., Hasegawa, K., Saba, R. and Saga, Y. Dev Cell, 34:96-107, 2015
DNA methylation and gene expression dynamics during spermatogonial stem cell differentiation in the early postnatal mouse testis. Kubo N, Toh H, Shirane K, Shirakawa T, Kobayashi H, Sato T, Sone H, Sato Y, Tomizawa S, Tsurusaki Y, Shibata H, Saitsu H, Suzuki Y, Matsumoto N, Suyama M, Kono T, Ohbo K, Sasaki H. BMC Genomics, 216:624, 2015
EPC1/TIP60-Mediated Histone Acetylation Facilitates Spermiogenesis in Mice. Dong Y, Isono KI, Ohbo K, Endo TA, Ohara O, Maekawa M, Toyama Y, Ito C, Toshimori K, Helin K, Ogonuki N, Inoue K, Ogura A, Yamagata K, Kitabayashi I, Koseki H. Mol Cell Biol., 2017 37(19)e00082-17
SHISA6 confers resistance to differentiation-promoting Wnt/β-catenin signaling in mouse spermatogenic stem cells. Tokue M, Ikami K, Mizuno S, Takagi C, Miyagi A, Takada R, Noda C, Kitadate Y, Hara K, Mizuguchi H, Sato T, Taketo MM, Sugiyama F, Ogawa T, Kobayashi S, Ueno N, Takahashi S, Takada S, Yoshida S*. Stem Cell Reports, 2017 Mar 14;8(3):561-575 >> NIBB ニュース
Genome-Scale Assessment of Age-Related DNA Methylation Changes in Mouse Spermatozoa. Kobayashi N, Okae H, Hiura H, Chiba H, Shirakata Y, Hara K, Tanemura K, Arima T*. PLoS One, 2016 Nov 23;11(11):e0167127
Exogenous neurotensin modulates sperm function in Japanese Black cattle. Umezu K, Hiradate Y, Oikawa T, Ishiguro H, Numabe T, Hara K, Tanemura K*. J Reprod Dev., 2016 Aug 25;62(4):409-14.
Hierarchical differentiation competence in response to retinoic acid ensures stem cell maintenance during mouse spermatogenesis. Ikami K, Tokue M, Sugimoto R, Noda C, Kobayashi S, Hara K, Yoshida S*. Development, 2015 May 1;142(9):1582-92 >> NIBB ニュース
Mouse spermatogenic stem cells continually interconvert between equipotent singly isolated and syncytial states. Hara K, Nakagawa T, Enomoto H, Suzuki M, Yamamoto M, Simons BD, Yoshida S*. Cell Stem Cell, 2014 May 1;14(5):658-72 >> NIBB ニュース
Spawning induction of blue mackerel Scomber australasicus and eastern little tuna Euthynnus affinis by oral administration of crude gonadotropin-releasing hormone analogue. Kotaro AMEZAWA, Ryosuke YAZAWA, Yutaka TAKEUCHI, Goro YOSHIZAKI Fisheries Science, (in press) (2018)
Production of functional sperm by subcutaneous auto-grafting of immature testes in rainbow trout. Makoto Hayashi, Daika Sakuma, Goro Yoshizaki Molecular Reproduction and Development, (in press) (2018)
Spawning induction and seed production of Eastern little tuna, Euthynnus affinis (Cantor, 1849), in the post- and pre-spawning seasons by hormonal treatment in a semi-closed recirculation system with elevated temperature. Yazawa R, Takecuhi Y, Satoh K, Machida Y, Amezawa K, Kabeya N, Yoshizaki G. Aquaculture Research, 48, 3472-2481 (2017)
Hypothermic storage of isolated spermatogonia and oogonia from rainbow trout (Oncorhynchus mykiss). Falahatkar B, Poursaeid S, Kitada R, Yoshizaki G.Cryobiology, 76, 125-128 (2017)
Efficient production of donor-derived gametes from triploid recipients following intra-peritoneal germ cell transplantation into a marine teleost, Nibe croaker (Nibea mitsukurii). Hiroyuki Yoshikawa, Yutaka Takeuchi, Yasuko Ino, Junjie Wang, Gaku Iwata, Naoki Kabeya, Ryosuke Yazawa, Goro Yoshizaki Aquaculture, 478, 35-47 (2017)
Stem cell activity of type A spermatogonia is seasonally regulated in rainbow trout: Mana Sato, Makoto Hayashi, Goro Yoshizaki Biology of Reproduction, 96(6), 1303-1316 (2017)
Flow-cytometric enrichment of Pacific bluefin tuna type A spermatogonia based on light- scattering properties: Kensuke Ichida, Kazuyoshi Kise, Tetsuro Morita, Ryosuke Yazawa , Yutaka Takeuchi, Goro Yoshizaki Theriogenology, 101, 91-98 (2017)
Production of tiger puffer Takifugu rubripes offspring from triploid grass puffer Takifugu niphobles parents. Masaomi Hamasaki*, Yutaka Takeuchi, Ryosuke Yazawa, Souta Yoshikawa, Kazushi Kadomura, Toshiyuki Yamada, Kadoo Miyaki, Kiyoshi Kikuchi and, Goro Yoshizaki Marine Biotechnology, 19, 579-591 (2017)
Application of dead end-knockout zebrafish as recipients of germ cell transplantation. Li Q, Fujii W, Naito K, Yoshizaki G.Mol Reprod Dev., 84, 1100-1111 (2017)
Production of the medaka derived from vitrified whole testes by germ cell transplantation.: Seki S, Kusano K, Lee S, Iwasaki Y, Yagisawa M,Ishida M, Hiratsuka T, Sasado T, Naruse K, Yoshizaki G Scientific Reports, 7, 43185 (2017)
Production of functionally sterile triploid Nibe croaker Nibea mitsukurii induced by cold-shock treatment with special emphasis on triploid aptitude as surrogate broodstock. Yutaka Takeuchi, Takashi Yatabe, Hiroyuki Yoshikawa, Yasuko Ino, Naoki Kabeya, Ryosuke Yazawa, Goro YoshizakiAquaculture (in press)
Characterization of a vasa homolog in the brown-marbled grouper(Epinephelus fuscoguttatus) and its expression in gonad and germ cells during larval development. S Boonanuntanasarn, P Bunlipatanon, K Ichida, K Yoohut, O Mengyu, S Detsathit, R Yazawa, G Yoshizaki Fish Physiology and Biochemistry, 42, 1621-1636 (2016)
Germ Cell-Specific Excision of loxP-flanked Transgenes in Rainbow Trout Oncorhynchus mykiss.: Naoto Katayama, Sachi Kume, Shoko Hattori-Ihara, Sakiko Sadaie, Makoto Hayashi and Goro Yoshizaki Biology of Reproduction, 94(4), 79 (2016)
Successful cryopreservation of spermatogonia in critically endangered Manchurian trout (Brachymystax lenok). Seungki Lee, Goro Yoshizaki Cryobiology, 72,(2), 165-168 (2016)
Production of germ cell-deficient salmonids by the dead end gene knockdown and their use as recipients for germ cell transplantation. Goro Yoshizaki, Kuniko Takashiba, Shotaro Shimamori, Kiyoko Fujinuma, Shinya Shikina, Tomoyuki Okutsu, Sachi Kume, Makoto Hayashi Molecular Reproduction and Development, 83, 298-311 (2016)
Long-term (5 years) cryopreserved spermatogonia have high capacity to generate functional gametes via interspecies transplantation in salmonids. Seungki Lee, Yoshiko Iwasaki, Goro Yoshizaki Cryobiology 73, 286-290 (2016)
Generation of juvenile rainbow trout derived from cryopreserved whole ovaries by intraperitoneal transplantation of ovarian germ cells. Lee S, Katayama N, Yoshizaki G. Biochem Biophys Res Commun., 478(3), 1478-1483 (2016)
Primordial germ cell migration in the yellowtail kingfish (Seriola lalandi) and identification of stromal cell-derived factor 1. J.A. Fernandez, E.J. Bubner, Y. Takeuchi, G. Yoshizaki, T. Wanga, S.F. Cummins, A. Elizur.Gen Comp Endocrinol, 213, 16-23 (2015)
Assessment of yellowtail kingfish (Seriola lalandi) as a surrogate host for the production of southern bluefin tuna (Thunnus maccoyii) seed via spermatogonial germ cell transplantation.: Ido Bar, Andre Smith, Erin Bubner, Goro Yoshizaki, Yutaka Takeuchi, Ryosuke Yazawa, Ben Nan Chen, Scott Cummins, Abigail Elizur Reproduction, Fertility and Development, 28(12), 2051-2064 (2015)
Small-scale capture, transport and tank adaptation of live, medium-sized Scombrids using “Tuna Tubes". Ido Bar, Luke Dutney, Peter Lee, Ryosuke Yazawa, Goro Yoshizaki, Yutaka Takeuchi,Scott Cummins, Abigail Elizur SpringerPlus, 4, 604 (2015)
Successful production of functional Y eggs derived from spermatogonia transplanted into female recipients and subsequent production of YY supermales in rainbowtrout, Oncorhynchus mykiss. T. Okutsu, S. Shikina, T. Sakamoto, M. Mochizuki, and G. Yoshizaki Aquaculture, 446, 298-302 (2015)
Functional sperm of the yellowtail (Seriola quinqueradiata) were produced in the small-bodied surrogate, jack mackerel (Trachurus japonicus). Tetsuro Morita, Kagayaki Morishima, Misako Miwa, Naoki Kumakura, Satomi Kudo, Kensuke Ichida, Toru Mitsuboshi, Yutaka Takeuchi, and Goro Yoshizaki Marine Biotechnology, 17(5), 644-654 (2015)
Production of viable trout offspring derived from frozen whole fish. Seungki Lee, Shinsuke Seki, Naoto Katayama & Goro Yoshizaki Scientific Reports, 5, 16045 (2015)
Intraperitoneal germ cell transplantation in the Nile tilapia Oreochromis niloticus. R. Farlora, S. Hattori-Ihara, Y. Takeuchi, M. Hayashi, A. Octavera, Alimuddin, G. Yoshizaki Marine Biotechnology, 16(3), 309-320 (2014)
Enrichment of Spermatogonial Stem Cells Using Side Population in Teleost. Makoto Hayashi, Masanao Sato, Yasuhiko Nagasaka, Sakiko Sadaie, Satoru Kobayashi, Goro Yoshizaki Biology of Reproduction, 91:23, 1-8 (2014)
Production of genetically diversified fish seeds using spermatogonial transplantation. M. Sato, T. Morita, N. Katayama, G. Yoshizaki Aquaculture 422-423, 218-224 (2014)
Expression patterns of gdnf and gfra1 in rainbow trout testis. S. Nakajima, M. Hayashi, T. Kouguchi, K. Yamaguchi, M. Miwa, G. Yoshizaki Gene Expression Patterns, 14, 111-120 (2014)
Identification and migration of primordial germ cells in Atlantic salmon (Salmo Salar) and Atlantic Cod (Gadus morhua). Nagasawa K, Presslauer C, Yoshizaki G, Miwa M, Fernandes JM, Babiak I Commun Agrid Appl Bio Sci, 78, 296-299 (2013)
The Pacific bluefin tuna (Thunnus orientalis) dead end gene is suitable as a specific molecular marker of type A spermatogonia. R. Yazawa, Y. Takeuchi, T. Morita, M. Ishida, G. Yoshizaki Mol Reprod Dev, 80(10), 871-880 (2013)
Gonadal development and fertility of triploid grass puffer takifugu niphobles induced by cold shock treatment. M. Hamasaki, Y. Takeuchi, K. Miyaki, G. Yoshizaki Mar. Biotechnol. 15(2), 133-144 (2013)
Germ cell transplantation as a potential biotechnological approach to fish. reproduction. SMSN. Lacerda, GMJ. Costa, PHA. Campos-Junior, TM. Segatelli, R. Yazawa, Y. Takeuchi, T. Morita, G. Yoshizaki, LR. França Fish Physiology and Biochemistry, 39, 3-11 (2013)
Short-term in vitro culturing improves transplantability of type A spermatogonia in rainbow trout (Oncorhynchus mykiss). S. Shikina, K. Nagasawa, M. Hayashi, M. Furuya, Y. Iwasaki, and G. Yoshizaki Molecular Reproduction & Development, 80(9), 763-773 (2013)
Generation of functional eggs and sperm from cryopreserved whole testes. S. Lee, Y. Iwasaki, S. Shikina, G. Yoshizaki. Proceedings of the National Academy of Sciences of the United States of America, 110(5), 1640-1645 (2013)
Identification and migration of primordial germ cells in Atlantic salmon, Salmo salar: Characterization of vasa, dead end, and lymphocyte antigen 75 genes. K. Nagasawa, J.M. Fernandes, G. Yoshizaki, M. Miwa, I. Babiak. Mol Reprod Dev., 80(2), 118-131 (2013)
Bone morphogenetic protein and retinoic acid synergistically specify female germ-cell fate in mice. Miyauchi H, Ohta H, Nagaoka S, Nakaki F, Sasaki K, Hayashi K, Yabuta Y, Nakamura T, Yamamoto T, Saitou M*. EMBO J., 2017 36:3100-3119.
Reconstitution of mouse oogenesis in a dish from pluripotent stem cells. Hayashi K*, Hikabe O, Obata Y, Hirao Y. Nat Protoc., 2017 12:1733-1744.
PCGF6-PRC1 suppresses premature differentiation of mouse embryonic stem cells by regulating germ cell-related genes. Endoh M*, Endo TA, Shinga J, Hayashi K, Farcas A, Ma KW, Ito S, Sharif J, Endoh T, Onaga N, Nakayama M, Ishikura T, Masui O, Kessler BM, Suda T, Ohara O, Okuda A, Klose R, Koseki H*. Elife 2017 Mar 17;6. pii: e21064.
In Vitro Derivation and Propagation of Spermatogonial Stem Cell Activity from Mouse Pluripotent Stem Cells. Ishikura Y, Yabuta Y, Ohta H, Hayashi K, Nakamura T, Okamoto I, Yamamoto T, Kurimoto K, Shirane K, Sasaki H, Saitou M*. Cell Rep., 2016 Dec 6;17(10):2789-2804
Reconstitution in vitro of the entire cycle of the mouse female germ line. Hikabe O, Hamazaki N, Nagamatsu G, Obata Y, Hirao Y, Hamada N, Shimamoto S, Imamura T, Nakashima K, Saitou M, Hayashi K*. Nature, 2016 Nov 10;539(7628):299-303
※配偶子産生のサイクルを培養下で再現することに成功
Global Landscape and Regulatory Principles of DNA Methylation Reprogramming for Germ Cell Specification by Mouse Pluripotent Stem Cells. Shirane K, Kurimoto K, Yabuta Y, Yamaji M, Satoh J, Ito S, Watanabe A, Hayashi K, Saitou M*, Sasaki H*. Dev Cell, 2016 39:87-103
Complete in vitro generation of fertile oocytes from mouse primordial germ cells. Morohaku K, Tanimoto R, Sasaki K, Kawahara-Miki R, Kono T, Hayashi K, Hirao Y*, Obata Y*. Proc Natl Acad Sci U S A. 2016 Aug 9;113(32):9021-6
Rewinding the process of mammalian extinction. Saragusty J, Diecke S, Drukker M, Durrant B, Friedrich Ben-Nun I, Galli C, Göritz F, Hayashi K, Hermes R, Holtze S, Johnson S, Lazzari G, Loi P, Loring JF, Okita K, Renfree MB, Seet S, Voracek T, Stejskal J, Ryder OA*, Hildebrandt TB*. Zoo Biol., 2016 Jul;35(4):280-92.
Quantitative Dynamics of Chromatin Remodeling during Germ Cell Specification from Mouse Embryonic Stem Cells. Kurimoto K*, Yabuta Y, Hayashi K, Ohta H, Kiyonari H, Mitani T, Moritoki Y, Kohri K, Kimura H, Yamamoto T, Katou Y, Shirahige K, Saitou M*. Cell Stem Cel,l 2015 May 7;16(5):517-32
Induction of primordial germ cell-like cells from mouse embryonic stem cells by ERK signal inhibition. Kimura T*, Kaga Y, Ohta H, Odamoto M, Sekita Y, Li K, Yamano N, Fujikawa K, Isotani A, Sasaki N, Toyoda M, Hayashi K, Okabe M, Shinohara T, Saitou M, Nakano T. Stem Cells, 2014 Oct;32(10):2668-78.
A mesodermal factor, T, specifies mouse germ cell fate by directly activating germline determinants. Aramaki S, Hayashi K, Kurimoto K, Ohta H, Yabuta Y, Iwanari H, Mochizuki Y, Hamakubo T, Kato Y, Shirahige K, Saitou M*. Dev Cell, 2013 Dec 9;27(5):516-29
Tsix RNA and the germline factor, PRDM14, link X reactivation and stem cell reprogramming. Payer B, Rosenberg M, Yamaji M, Yabuta Y, Koyanagi-Aoi M, Hayashi K, Yamanaka S, Saitou M, Lee JT*. Mol Cell, 2013 Dec 26;52(6):805-18
Induction of mouse germ-cell fate by transcription factors in vitro. Nakaki F, Hayashi K, Ohta H, Kurimoto K, Yabuta Y, Saitou M*. Nature, 2013 Sep 12;501(7466):222-6
Generation of eggs from mouse embryonic stem cells and induced pluripotent stem cells. Hayashi K*, Saitou M*. Nat Protoc., 2013 Aug;8(8):1513-24
※ES細胞やiPS細胞などの多能性幹細胞からの始原生殖細胞の分化、およびそれらの移植による卵子の作製について、詳細な実験手順を報告した。 >> 詳細
PRDM14 ensures naive pluripotency through dual regulation of signaling and epigenetic pathways in mouse embryonic stem cells. Yamaji M, Ueda J, Hayashi K, Ohta H, Yabuta Y, Kurimoto K, Nakato R, Yamada Y, Shirahige K, Saitou M*. Cell Stem Cell, 2013 Mar 7;12(3):368-82
Pumpless microfluidic system driven by hydrostatic pressure induces and maintains mouse spermatogenesis in vitro. Komeya M, Hayashi K, Nakamura H, Yamanaka H, Sanjo H, Kojima K, Sato T, Yao M, Kimura H, Fujii T, Ogawa T* Sci Rep., 2017 Nov 13;7(1):15459
A noncoding RNA containing a SINE-B1 motif associates with meiotic metaphase chromatin and has an indispensable function during spermatogenesis. Nakajima R, Sato T, Ogawa T, Okano H, Noce T. PLoS One, 2017 Jun 28;12(6):e0179585.
Evaluation of Culture Time and Media in an In Vitro Testis Organ Culture System. Nakamura N, Merry GE, Inselman AL, Sloper DT, Del Valle PL, Sato T, Ogawa T, Hansen DK. Birth Defects Res, 2017 Apr 17;109(7):465-474
Spermatogenesis in Explanted Fetal Mouse Testis Tissues. Kojima K, Sato T, Naruse Y, Ogawa T* Biol Reprod, 2016 Sep;95(3):63, 1-6. Epub 2016 Jul 14.
Evaluation of Culture Time and Media in an In Vitro Testis Organ Culture System. Nakamura N, Merry GE, Inselman AL, Sloper DT, Del Valle PL, Sato T, Ogawa T, Hansen DK. Birth Defects Res, 2017 Apr 17;109(7):465-474
Long-term ex vivo maintenance of testis tissues producing fertile sperm in a microfluidic device. Komeya M, Kimura H, Nakamura H, Yokonishi T, Sato T, Kojima K, Hayashi K, Katagiri K, Yamanaka H, Sanjo H, Yao M, Kamimura S, Inoue K, Ogonuki N, Ogura A, Fujii T, Ogawa T.* Scientific Reports, Feb 19; 6: 21472. (2016)
In Vitro Spermatogenesis in Explanted Adult Mouse Testis Tissues. Sato T, Katagiri K, Kojima K, Komeya M, Yao M, Ogawa T* PLoS One, 2015 Jun 12;10(6):e0130171.
Genome Editing in Mouse Spermatogonial Stem Cell Lines Using TALEN and Double-Nicking CRISPR/Cas9. Sato T*, Sakuma T, Yokonishi T, Katagiri K, Kamimura S, Ogonuki N, Ogura A, Yamamoto T, Ogawa T* Stem Cell Reports, 2015 Jul 14;5(1):75-82.
Offspring production with sperm grown in vitro from cryopreserved testis tissues. Yokonishi T, Sato T, Komeya M, Katagiri K, Kubota Y, Nakabayashi K, Hata K, Inoue K, Ogonuki N, Ogura A, Ogawa T* Nat Commun., 2014 Jul 1;5: 4320. doi: 10.1038/ncomms5320.
※凍結保存したマウス精巣組織片からの精子産生と産仔に成功！ >> 横浜市立大学 研究成果
In vitro sperm production from mouse spermatogonial stem cell lines using an organ culture method. Sato T, Katagiri K, Kubota Y, Ogawa T* Nat Protoc., 2013 Nov; 8(11):2098-2104
※培養精子幹細胞株(GS細胞)からの体外での精子形成誘導法の詳細な実験手順を記載 >> 詳細
In Vitro Reconstruction of Mouse Seminiferous Tubules Supporting Germ Cell Differentiation. Yokonishi T, Sato T, Katagiri K, Komeya M, Kubota Y, Ogawa T* Biol Reprod., July 1, 2013, 89(1): 15, 1-6.
In vitro spermatogenesis using an organ culture technique. Yokonishi T, Sato T, Katagiri K, Ogawa T* Methods Mol Biol., 2013;927:479-488.
A High-Throughput Device for Patterned Differentiation of Embryoid Bodies. X. He, H. Kimura and T. Fujii* Journal of Robotics and Mechatronics, 25(4), 623-630 (2013)
精子選別機能集積型受精卵作出デバイスの開発. 高橋翼，中村寛子，木村啓志* 日本機械学会論文集，83(850),16-00560 (2017)
Neonatal testis growth recreated in vitro by two-dimensional organ-spreading. K. Kojima, H. Nakamura, M. Komeya, H. Yamanaka, Y. Makino, Y. Okada, T. Sato, T. Fujii, H. Kimura and T. Ogawa* Biotechnology and Bioengineering, (2018) in press
Macromolecular crowded conditions strengthen contacts between mouse oocytes and companion granulosa cells during growth in vitro. S. Mizumachi, T. Aritomi, K. Sasaki, K. Matsubara, Y. Hirao* J Reprod Dev., 64, in press (2018)
Development of fertile mouse oocytes from mitotic germ cells in vitro. K. Morohaku, Y. Hirao, Y. Obata* Nat Protoc., 12, 1817-1829 (2017)
Differentiation of Mouse Primordial Germ Cells into Functional Oocytes In Vitro. K. Morohaku, Y. Hirao, Y. Obata* Ann Biomed Eng., 45, 1608-1619 (2017)
Complete in vitro generation of fertile oocytes from mouse primordial germ cells. K. Morohaku, R. Tanimoto, K. Sasaki, R. Kawahara-Miki, T. Kono, K. Hayashi, Y. Hirao*, Y. Obata* Proc Natl Acad Sci U S A 113, 9021-9026 (2016)
※始原生殖細胞から機能的な卵子産生までを試験管内で再現する悲願を達成！
Developmental competence of oocytes grown in vitro: Has it peaked already? K. Morohaku, Y. Hirao, Y. Obata* J Reprod Dev., 62, 1-5 (2016)
Establishment of a conditional transgenic system using the 2A peptide in the female mouse germline. S. Hara, T. Takano, M. Ogata, R. Yamakami, Y. Sato, T. Kono, Y. Obata* J Reprod Dev., 60, 250-255 (2014)
Forced expression of DNA methyltransferases during oocyte growth accelerates the establishment of methylation imprints but not functional genomic imprinting. S. Hara, T. Takano, T. Fujikawa, M. Yamada, T. Wakai, T. Kono, Y. Obata* Hum Mol Genet, 23, 3853-2864 (2014)
Long exposure to mature ooplasm can alter DNA methylation at imprinted loci in non-growing oocytes but not in prospermatogonia. Y. Obata*, T. Wakai, S. Hara, T. Kono Reproduction, 147, H1-6 (2014)
Regionally distinct patterns of STAT3 phosphorylation in the seminiferous epithelia of mouse testes. Nagasawa K, Imura-Kishi K, Uchida A, Hiramatsu R, Kurohmaru M, Kanai Y* Mol Reprod Dev, in press (2018)
Germ cell-intrinsic requirement for the homeodomain transcription factor PKnox1/Prep1 in adult spermatogenesis. Kawai Y, Oda A, Kanai Y, Goitsuka R* PLoS One, 13:e0190702 (2018)
Spermatogonial deubiquitinase USP9X is essential for proper spermatogenesis in mice. Kishi K, Uchida A, Takase HM, Suzuki H, Kurohmaru M, Tsunekawa N, Kanai-Azuma M, Wood SA, Kanai Y* Reproduction, 154:135-143 (2017)
Defects in the first wave of folliculogenesis in mouse XO ovaries. Miura K, Murata C, Harikae K, Suzuki H, Kanai-Azuma M, Kurohmaru M, Tsunekawa N, Kanai Y* J Reprod Dev., 63:333-338 (2017)
Differential lactate and cholesterol synthetic activities in XY and XX Sertoli cells. Shishido Y, Baba T, Sato T, Shima Y, Miyabayashi K, Inoue M, Akiyama H, Kimura H, Kanai Y, Ishihara Y, Haraguchi S, Miyazaki A, Rozman D, Yamazaki T, Choi MH, Ohkawa Y, Suyama M, Morohashi KI* Sci Rep., 2017 7:41912 (2017)
Data on in vivo phenotypes of GFRα1-positive spermatogonia stimulated by interstitial GDNF signals in mouse testes. Uchida A, Kanai Y* Data Brief. 8:1255-8 (2016)
In vivo dynamics of GFRα1-positive spermatogonia stimulated by GDNF signals using a bead transplantation assay. Uchida A, Kishi K, Aiyama Y, Miura K, Takase HM, Suzuki H, Kanai-Azuma M, Iwamori T, Kurohmaru M, Tsunekawa N, Kanai Y* Biochem Biophys Res Commun., 476:546-552 (2016)
Mouse Sox17 haploinsufficiency leads to female subfertility due to impaired implantation. Hirate Y, Suzuki H, Kawasumi M, Takase HM, Igarashi H, Naquet P, Kanai Y, Kanai-Azuma M* Sci Rep., 6:24171 (2016)
A Niche for GFRα1-Positive Spermatogonia in the Terminal Segments of the Seminiferous Tubules in Hamster Testes. Aiyama Y, Tsunekawa N, Kishi K, Kawasumi M, Suzuki H, Kanai-Azuma M, Kurohmaru M, Kanai Y* Stem Cells, 33:2811-24 (2015)
Single administration of butylparaben induces spermatogenic cell apoptosis in prepubertal rats. Alam MS, Ohsako S, Kanai Y, Kurohmaru M* Acta Histochem., 116:474-80 (2014)
A novel Amh-Treck transgenic mouse line allows toxin-dependent loss of supporting cells in gonads. Shinomura M, Kishi K, Tomita A, Kawasumi M, Kanezashi H, Kuroda Y, Tsunekawa N, Ozawa A, Aiyama Y, Yoneda A, Suzuki H, Saito M, Picard JY, Kohno K, Kurohmaru M, Kanai-Azuma M, Kanai Y* Reproduction, 148:H1-9. (2014)
Dead end1 is an essential partner of NANOS2 for selective binding of target RNAs in male germ cell development. Suzuki A*, Niimi Y, Shinmyozu K, Zhou Z, Kiso M, Saga Y* EMBO Reports, 2016 Jan;17(1):37-46
Identification of KIAA1210 as a novel X-chromosome-linked protein that localizes to the acrosome and associates with the ectoplasmic specialization in testes. Iwamori T*, Iwamori N, Matsumoto M, Ono E, Matzuk MM Biol Reprod., 2017 Feb 1;96(2):469-477
Endophilin B is required for the Drosophila oocyte to endocytose yolk downstream of Oskar. Tsai, Y. C., Chang, W., Liou, W., Lee, W. H., Chang, Y. W., Wang, P. Y., Tanaka, T., Nakamura, A. and *Pai, L. M. Development, 141, 563-573 (2014)
Region-specific activation of oskar mRNA translation by inhibition of Bruno-mediated repression. Kim, G., Pai, C.-I., Sato, K., Person, M. D., Nakamura, A. and *Macdonald, P. M. PLoS Genet, 11, e1004992 (2015)
Efficient endocytic uptake and maturation in Drosophila oocytes requires Dynamitin/p50. Liu, G., Sanghavi, P., Bollinger, K. E., Perry, L., Marshall, B., Roon, P., Tanaka, T., Nakamura, A. and *Gonsalvez, G. B. Genetics, 201, 631-649 (2015)
Successful fertility preservation following ovarian tissue vitrification in patients with primary ovarian insufficiency. Suzuki N, Yoshioka N, Takae S, Sugishita Y, Tamura M, Hashimoto S, Morimoto Y, Kawamura K* Hum Reprod., 30, 608-615, 2015
Intraovarian control of early folliculogenesis. Hsueh AJ*, Kawamura K, Cheng Y, Fauser BC Endocr Rev., 2015 Feb;36(1):1-24
Actin polymerization - enhancing drugs promote ovarian follicle growth mediated by the Hippo signaling effector YAP.. Cheng Y, Feng Y, Jansson L, Sato Y, Deguchi M, Kawamura K, Hsueh AJ* FASEB J., 2015 Jun;29(6):2423-30
Ovary transplantation: to activate or not to activate. Kawamura K*, Cheng Y, Sun YP, Zhai J, Diaz-Garcia C, Simon C, Pellicer A, Hsueh AJ Hum Reprod., 2015 Nov;30(11):2457-60
Activation of dormant follicles: a new treatment for premature ovarian failure?. Kawamura K*, Kawamura N, Hsueh AJ Curr Opin Obstet Gynecol., 2016 Jun;28(3):217-22
In Vitro Activation of Follicles and Fresh Tissue Auto-transplantation in Primary Ovarian Insufficiency Patients. Zhai J, Yao G, Dong F, Bu Z, Cheng Y, Sato Y, Hu L, Zhang Y, Wang J, Dai S, Li J, Sun J, Hsueh AJ, Kawamura K, Sun Y* J Clin Endocrinol Metab., 2016 Nov;101(11):4405-4412
Determination of Follicular Localization in Human Ovarian Cortex for Vitrification. Haino T, Tarumi W, Kawamura K*, Harada T, Sugimoto K, Okamoto A, Ikegami M, Suzuki N J Adolesc Young Adult Oncol., 2017 Aug 28 doi: 10.1089/jayao.2017.0028
Disorganization of the germ cell pool leads to primary ovarian insufficiency. Kawashima I, Kawamura K* Reproduction, 2017 Jun;153(6):R205-R213
Regulation of follicle growth through hormonal factors and mechanical cues mediated by Hippo signaling pathway. Kawashima I, Kawamura K* Syst Biol Reprod Med., 2018 Feb;64(1):3-11
早発卵巣不全患者の卵巣刺激に対する当院のプロトコールの有用性 採卵率と早期黄体化の検討. 川越雄太*、河村和弘、星名真理子、水町静華、西島千絵、吉岡伸人、高江正道、洞下由紀、杉下陽堂、田中守、鈴木直、石塚文平 日本IVF学会誌 17: 48-52, 2014
Molecular cloning of chicken TET family genes and role of chicken TET1 in erythropoiesis. Yuya Okuzaki, Hidenori Kaneoka*, Ken-Ichi Nishijima, Seitaro Murakami, Yuki Ozawa, Shinji Iijima Biochemical and Biophysical Research Communications, 490(3):753-759 (2017)
Characterization of chicken interferon-inducible transmembrane protein-10. Yuya Okuzaki, Shunsuke Kidani, Hidenori Kaneoka, Shinji Iijima and Ken-ichi Nishijima* Bioscience, Biotechnology and Biochemistry, 81(5), 914-921 (2017)
Expression of interferon-inducible transmembrane proteins in the chicken and possible role in prevention of viral infections. Shunsuke Kidani, Yuya Okuzaki, Hidenori Kaneoka, Seiya Asai, Seitaro Murakami, Yusuke Murase, Shinji Iijima, Ken-ichi Nishijima* Cytotechnology, 69(3):477-484 (2017)
E ects of cadmium exposure on Iberian ribbed newt (Pleurodeles waltl) testes. A. Hirako, Y. Takeoka, T. Hayashi, T. Takeuchi, S. Furukawa, and A. Sugiyama* J Toxicol Pathol, 30:345–350. (2017)
Neuregulin 1 Regulates Proliferation of Leydig Cells to Support Spermatogenesis and Sexual Behavior in Adult Mice. Umehara T, Kawashima I, Kawai T, Hoshino Y, Morohashi KI, Shima Y, Zeng W, Richards JS, Shimada M*. Endocrinology 157:4899-4913, 2016
SF-1 deficiency causes lipid accumulation in Leydig cells via suppression of STAR and CYP11A1. Hatano M, Migita T*, Ohishi T, Shima Y, Ogawa Y, Morohashi KI, Hasegawa Y, Shibasaki F. Endocrine 54:484-496, 2016
Alterations in Fetal Leydig Cell Gene Expression during Fetal and Adult Development. Miyabayashi K, Shima Y, Inoue M, Sato T, Baba T, Ohkawa Y, Suyama M, Morohashi KI*. Sex Dev., 11:53-63, 2017
Differential lactate and cholesterol synthetic activities in XY and XX Sertoli cells. Shishido Y, Baba T, Sato T, Shima Y, Miyabayashi K, Inoue M, Akiyama H, Kimura H, Kanai Y, Ishihara Y, Haraguchi S, Miyazaki A, Rozman D, Yamazaki T, Choi MH, Ohkawa Y, Suyama M, Morohashi KI*. Sci Rep., 7:41912, 2017
Role of Ad4-binding protein/steroidogenic factor 1 in regulating NADPH production in adrenocortical Y-1 cells. Li B, Baba T, Miyabayashi K, Sato T, Shima Y, Ichinose T, Miura D, Ohkawa Y, Suyama M, Morohashi KI*. Endocr J., 64:315-324, 2017
Expression analysis of the endogenous Zscan4 locus and its coding proteins in mouse ES cells and preimplantation embryos. Ishiguro K., Nakatake Y., Chikazawa-Nohtomi N., Kimura H., Akiyama T., Oda M., Ko SBH., Ko MSH* In Vitro Cell.Dev.Biol.-Anim. 53, 179-190 (2017)
Zscan4 is expressed specifically during late meiotic prophase in both spermatogenesis and oogenesis: Ishiguro K., Monti M., Akiyama T., Kimura H., Chikazawa-Nohtomi N., Sakota M., Sato S., Redi CA., Ko SBH., Ko MSH* In Vitro Cell.Dev.Biol. -Anim. 53, 167-178 (2017)
PRDM14 drives OCT3/4 recruitment via active demethylation in the transition from primed to naïve pluripotency. Okashita, N., Suwa Y., Nishimura O., Sakashita N., Kadota M., Nagamatsu G., Kawaguchi M., Kashida H., Nakajima A., Tachibana M., Seki Y.* Stem Cell Reports, 7(6), 1072-1086, 2016