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National Institutes of Natural Sciences

National Institute for Basic Biology

NIBB Departments

Researcher Infomation

YOSHIDA, Takuya
Specially appointed Associate Professor
YOSHIDA, Takuya
Affiliation:

Research Summary

Mass spectrometry-based omics analyses have become indispensable tools in modern molecular biology. Our research group focuses on unraveling plant signaling pathways through comprehensive proteomic and metabolomic profiling, with mass spectrometry at the core of our approach. A key area of our work is the study of phytohormones—crucial signaling molecules that regulate nearly all aspects of plant growth, development, and stress responses. Specifically, we are investigating the signaling pathways of abscisic acid (ABA) to understand how plants, particularly Arabidopsis, fine-tune growth and stress responses.

The Trans-Omics Facility serves as a core research hub, supporting a wide network of scientists both within and beyond NIBB. To cater to diverse research needs, we are actively developing cutting-edge analytical methods and protocols for mass spectrometry-based proteomics and metabolomics.
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Advanced Mass Spectrometry Applications in Multi-Omics Research.

Research Projects

  • • Implementing Mass Spectrometry Imaging with timsTOF fleX (Bruker)
  • • Developing Data-Independent Acquisition (DIA) Mass Spectrometry Protocols for Proteomic Profiling
  • • Unraveling Plant Growth and Stress Response Mechanisms through Phytohormone Signaling, with a Focus on Abscisic Acid (ABA)

Selected Publications

Yoshida, T.*, Mergner, J., Yang, Z., Liu, J., Kuster, B., Fernie, A.R., and Grill, E.* (2024), Integrating multi-omics data reveals energy and stress signaling activated by abscisic acid in Arabidopsis. Plant J., 119, 1112-1133. *co-coresponding authors. https://doi.org/10.1111/tpj.16765

Jiang, L.†, Yoshida, T.†, Stiegert, S., Jing, Y., Alseekh, S., Lenhard, M., Pérez-Alfocea, F., and Fernie, A.R. (2021), Multi-omics approach reveals the contribution of KLU to leaf longevity and drought tolerance. Plant Physiol., 185, 352-368. †These authors contributed equally. https://doi.org/10.1093/plphys/kiaa034

Yoshida, T., Obata, T., Feil, R., Lunn, J.E., Fujita, Y., Yamaguchi-Shinozaki, K., and Fernie, A.R. (2019), The Role of Abscisic Acid Signaling in Maintaining the Metabolic Balance Required for Arabidopsis Growth under Nonstress Conditions. Plant Cell, 31, 84-105. https://doi.org/10.1105/tpc.18.00766.

Review Articles
Yoshida, T.*, and  Fernie, A.R. (2024), Hormonal regulation of plant primary metabolism under drought. J. Exp. Bot., 75, 1714-1725. *corresponding author. https://doi.org/10.1093/jxb/erad358

Fàbregas, N.†, Yoshida, T.†, and  Fernie, A.R. (2020), Role of Raf-like kinases in SnRK2 activation and osmotic stress response in plants. Nat. Commun., 11, 6184. †These authors contributed equally. https://doi.org/10.1038/s41467-020-19977-2.

Yoshida, T.*, Christmann, A., Yamaguchi-Shinozaki, K., Grill, E., and  Fernie, A.R. (2019), Revisiting the Basal Role of ABA - Roles Outside of Stress. Trends Plant Sci., 24, 625-635. *corresponding author. https://doi.org/10.1016/j.tplants.2019.04.008