National Institute for Basic Biology
(Written in 2015)
Environmental factors including synthetic chemicals found in the environment have the capacity to disrupt sex-determination, sex-differentiation, development and reproduction in wildlife. These chemicals may bind to steroid hormone receptors and/or nuclear receptors and evoke hormonal/anti-hormonal effects and unpredictable effects, respectively. Thus, we are currently studying 1) molecular mechanisms of environmental estrogens and sex hormones act during critical developmental windows, 2) microarray analysis of estrogen responsive genes, 3) phylogeny of estrogen receptors, 4) temperature-dependent sex determination of American alligators, 5) male production in Daphnids by juvenile hormones, and 6) gene zoo, collection of gene information of various animal species.
Miyakawa, H., Toyota, K., Hirakawa, I., Ogino, Y., Miyagawa, S., Oda, S., Tatarazako, N., Miura, T., Colbourne, J.K. and Iguchi, T. (2013). A mutation in the Methoprene tolerant alters juvenile hormone response in insects and crustaceans. Nature Commun., 4, 1856.
Toyota, K., Kato, Y., Sato, M., Sugiura, N., Miyagawa, S., Miyakawa, H., Watanabe, H., Oda, S., Ogino, Y., Hiruta, C., Mizutani, T., Tatarazako, N., Paland, S., Jackson, C., Colbourne, J.K. and Iguchi, T. (2013). Molecular cloning of doublesex genes of four cladocera (water flea) species. BMC Genomics, 14, 239.
Bergman, Å, Heindel, J.J., Kidd, K.A., Jobling, S., Zoeller, R.T., Becher, G., Bjerregaard, P., Bornman, R., Brandt, I., Brian, J.V., Kortenkamp, A., Muir, D., Ochieng, R., Skakkebaek, N.E., Iguchi, T., Toppari, J. and Woodruff, T.J. (2013). The impact of endocrine disruption: A consensus statement on the state of the science. Environ. Health Perspect., 121, A104-106.
Guillette, L.J.Jr. and Iguchi, T. (2012). Life in a contaminated world. Science, 337, 1614-1615.
Oka, K., Kohno, S., Uruchitani, H., Guillette, L.J.Jr., Ohta, Y., Iguchi, T. and Katsu, Y. (2012). Molecular cloning and characterization of the corticoid receptors from the American alligator. Mol. Cell. Endocrinol., 365, 153-161.
Taylor, J.A., Richter, C.A., Suzuki, A., Watanabe, H., Iguchi, T., Coser, K.R., Shioda, T. and vom Saal, F.S. (2012). Dose-related estrogen effects on gene expression in fetal mouse prostate mesenchymal cells. PLoS One, 7(10): e48311.
Nakamura, T., Miyagawa, S., Katsu, Y., Watanabe, H., Mizutani, T., Sato, T., Morohashi, K.-I., Takeuchi, T., Iguchi, T. and Ohta, Y. (2012). WNT family genes and their modulation in the ovary-independent and persistent vaginal epithelial cell proliferation and keratinization induced by neonatal diethylstilbestrol exposure in mice. Toxicology, 296, 13-19.
Lange, A., Katsu, Y., Miyagawa, S., Ogino, Y., Urushitani, H., Kobayashi, T., Hirai, T., Shears, J.A., Nagae, M., Yamamoto, J., Ohnishi, Y., Oka, T., Tatarazako, N., Ohta, Y., Tyler, C.R. and Iguchi, T. (2012). Comparative responsiveness to natural and synthetic estrogens of fish species commonly used in the laboratory and field monitoring. Aquat. Toxicol., 109, 250-258.
Hirakawa, I., Miyagawa, S., Katsu, Y., Kagami, Y., Tatarazako, N., Kobayashi, T., Kusano, T., Mizutani, T., Ogino, Y., Takeuchi, T., Ohta, Y. and Iguchi, T. (2012). Gene expression profiles in the testis associated with testis-ova in adult Japanese medaka (Oryzias latipes) exposed to 17a-ethinylestradiol. Chemosphere, 87, 668-674.
St John, J.A., Braun, E.L., Isberg, S.R., Miles, L.G., Chong, A.Y., Gongora, J., Dalzell, P., Moran, C., Bed'hom, B., Abzhanov, A., Burgess, S.C., Cooksey, A.M., Castoe, T.A., Crawford, N.G., Densmore, L.D., Drew, J.C., Edwards, S.V., Faircloth, B.C., Fujita, M.K., Greenwold, M.J., Hoffmann, F.G., Howard, J.M., Iguchi, T., Janes, D.E., Khan, S.Y., Kohno, S., de Koning, A.J., Lance, S.L., McCarthy, F.M., McCormack, J.E., Merchant, M.E., Peterson, D.G., Pollock, D.D., Pourmand, N., Raney, B.J., Roessler, K.A., Sanford, J.R., Sawyer, R.H., Schmidt, C.J., Triplett, E.W., Tuberville, T.D., Venegas-Anaya, M., Howard, J.T., Jarvis, E.D., Guillette, L.J.Jr., Glenn, T.C., Green, R.E. and Ray, D.A. (2012). Sequencing three crocodilian genomes to illuminate the evolution of archosaurs and amniotes. Genome Biol., 13, 415.
Miyagawa, S., Matsumaru, D., Murashima, A., Omori, A., Satoh, Y., Haraguchi, R., Motoyama, J., Iguchi, T., Nakagata, N., Hui, C.C. and Yamada, G. (2011). The role of sonic hedgehog-Gli2 pathway in the masculinization of external genitalia. Endocrinology, 152, 2894-2903.
Ogino, Y., Miyagawa, S., Katoh, H., Prins, G.S., Iguchi, T. and Yamada, G. (2011). Essential functions of androgen signaling emerged through the developmental analysis of vertebrate sex characteristics. Evol. Devel., 13, 315-325.
Kato, Y., Kobayashi, K., Watanabe, H. and Iguchi, T. (2011). Environmental sex determination in the branchiopod crustacean Daphnia magna: Deep conservation of a Doublesex gene in the sex-determining pathway. PLoS Genetics, 7, e1001345.
