Flowering plants have evolved from plants without flowers. It is known that the function of several genes, called MADS-box genes, creates shapes peculiar to flowers such as stamens, pistils, and petals. Although plants that do not produce flowers, such as mosses, ferns, and green algae are also known to have the MADS-box genes. However, it was not well understood how the MADS-box genes work in plants without flowers until now. In order to understand the mechanism of flower evolution, it is necessary to understand how the MADS-box genes work in plants without flowers.
A research team led by Professor Mitsuyasu Hasebe of the National Institute for Basic Biology revealed that the MADS-box genes control sperm motility and cell division and elongation of the stem of gametophores, using the moss Physcomitrella patens. Graduate student Shizuka Koshimizu of the research team said, "There are six MADS-box genes in Physcomitrella patens, and we analyzed their functions using moss in which we broke those six genes. In moss which lost the function of all MADS-box genes, sperm flagella hardly moved. Moreover, in the stem, the increase of the length prevented water supply to the tip, in which sperm swim for fertilization. The MADS-box genes are critical for fertilization in two ways: providing enough water for sperm swimming and producing movable flagella."
Professor Hasebe said, "Both the gametophore and sperm flagella have been lost in the process of evolution as the flowering plants adapted to the dry environment on land. Based on this, it is likely that the MADS-box genes that worked in the gametophore and sperm flagella became unnecessary, and that the flower might have evolved by reusing them for other functions. It is interesting that genetic regulatory networks of development are different between different lineages in plants, although they are relatively conserved in animals"
These research results were published in Nature Plants on January 3rd. This research was conducted as a collaborative research project by the National Institute for Basic Biology, SOKENDAI (the Graduate University for Advanced Studies), Kanazawa University, Tokyo Institute of Technology, Miyagi University, and The University of Tokyo.
A moss Physcomitrella patens in which six MADS-box genes are disrupted (right) forms a more elongated shoot than the normal moss does (left). This results in the prohibition of water uptake from the base to the top.
“Physcomitrella MADS-box genes regulate water supply and sperm movement for fertilization”
Shizuka Koshimizu, Rumiko Kofuji, Yuko Sasaki-Sekimoto, Masahide Kikkawa, Mie Shimojima, Hiroyuki Ohta, Shuji Shigenobu, Yukiko Kabeya, Yuji Hiwatashi, Yosuke Tamada, Takashi Murata, and Mitsuyasu Hasebe