Introduction
The National Institute for Basic Biology (NIBB) is a sub-center of the National BioResource Project (NBRP) for work concerning Morning Glories. Our group works with the core institute at Kyushu University to gather, preserve, and distribute mutant lines for flower pigmentation and various DNA clones. Please contact Dr. Atsushi Hoshino for information on receiving or depositing resources. Please note our distribution is aimed only at those working within research institutes. Non academics should contact the core NBRP institute directly.
Contact: hoshino☆nibb.ac.jp (Please replace ☆ with @)
Core Institute：Kyushu University Faculty of Science (Morning Glory Page, Japanese)
NBRP Information Site：Morning Glory Database
NBRP Information Site：Morning Glory Project Information
NBRP Publication Database：Research Resource Circulation
Mutant Lines (Morning Glory Seeds)
We have 220 lines, mainly Ipomoea nil pigmentation mutants, in storage. We also maintain and distribute wild-type I. nil, and I. purpurea、I. tricolor, and other Ipomoea lines. Most of these strains have actively been used in research. Therefore, many of the mutant pigmentation genes have already been isolated and cloned.
Line list 1 (Japanese)
Lin2 list 2 (NBRP Site)
Phenotypes
EST Clones
We used a cDNA library from seedlings, and 3 cDNA libraries from flowers and buds of wild-type I. nil, also known as ‘Tokyo Kokei Standard’, to obtain EST sequences, and are now preserving over 60,000 EST clones. The DDBJ and other public database accession numbers for the 25,899 EST sequences obtained from flower and bud full-length cDNA are: BJ553078 to BJ578976. Accession numbers for the 36,336 EST sequences obtained from seedlings are: CJ736987 to CJ773322.
EST Database：http://ipomoeanil.nibb.ac.jp/
BAC Clones
We are preserving 105,216 BAC clones created from the ‘Tokyo Kokei Standard Line’. This covers the 800~1000 Mb Morning Glory genome 9 or 11 times. We also offer a 3D DNA pool for screening BAC DNA for PCR. With as few as 58 PCR procedures we can obtain our desired clone.
Vector Clones
Flower petal specific expression vectors were deposited by Dr. Shiratake of Nagoya University. Please click here for further details.
Vectors for medaka transgenesis were deposited by Dr. Anzai of Okayama University. Please click here for further details.
Distribution Procedures
Please contact Dr. Hoshino regarding how to place orders. After discussing your research goals appropriate recourses will be suggested. Once the order is placed we will ask for a Material Transfer Agreement (MTA) to be filled out. Also handling & shipping remuneration will be requested (only for actual expenses).
MTA (Japanese, Word File)
MTA（English, Word File）
Resource Distribution Process
Cost Breakdown
Since April 2010, we have requested reimbursement of the actual costs associated with the provision of bioresources, including consumables such as containers and tubes, personnel costs for packaging, shipping, and MTA processing, and postage. The requested bioresources themselves are provided free of charge. An invoice will be issued after the resources have been delivered. Please pay the costs listed in the following table by bank transfer. Bank transfer fees shall be borne by the requester.
Cost Breakdown |
Handling Fees (¥) |
|
| Seeds | Handling fees per request | 750 |
| Per line | 150 | |
| DNA Clones | Handling fees per request | 1,900 |
| Per clone | 500 | |
| Supply expense (per 10 clones) | 1,400 | |
| Screening Kits | Handling fees per request | 1,100 |
| Per kit | 7,500 | |
| Shipping Materials | Envelope (S) per envelope | 100 |
| Envelope (M) per envelope | 100 | |
| Envelope (L) per envelope | 150 | |
| Envelope (XL) per envelope | 300 | |
| *Shipping Costs | Actual Cost |
|
*Packages sent overseas are shipped via Express Mail Service.
For example, when requesting 10 seed lines, the total charge will be 2,350 yen, consisting of a 750 yen handling fee per resource request, a 1,500 yen fee for the 10 seed lines, and a 100 yen fee for shipping materials, such as a medium-sized envelope. Actual shipping costs will be added to this amount.
Supply charge regulations (PDF File, Japanese)
Supply implementation Regulations (PDF File, Japanese)）
Actual Cost reimbursement details (PDF File, Japanese)
Depositing Resources
We are actively collecting mutant lines of morning glories and their close relations, as well as various types of DNA clones and other genetically modified lines. We are particularly interested in collecting flower color variant mutant lines. Occasionally, new mutations occur among commercially available morning glories, or lines used in previous research are lost (such as when older garden varieties die out, or cease to be sold on the market). If you are in possession of any such new or lost seed lines, please contact us.
Contact: (MTA Postal Address)
Hoshino Atsushi
National Institute for Basic Biology Nishigonaka-38 Myōdaijicho Okazaki-shi, Aichi-ken, Japan, 444-8585
Email: hoshino☆nibb.ac.jp (Please replace ☆ with @)
Phone: +81-564-55-7534