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Introduction

The National Institute for Basic Biology (NIBB) is a sub-center of the National BioResource Project (NBRP) for work on Morning Glories. Our group works with the core institute at Kyushu University to gather, preserve, and distribute mutant lines for flower pigmentation and various DNA clones. Please contact Dr. Atsushi Hoshino for information on receiving or depositing resources. Please note our distribution is aimed only at those working within research institutes. Non academics should contact the core NBRP institute directly.

Contact: hoshino☆nibb.ac.jp (Please replace ☆ with @) 



Core Institute:Kyushu University Faculty of Science (Morning Glory Page, Japanese) 

NBRP Information Site:Morning Glory Page
NBRP Publication Database:Research Resource Circulation

mg-banner

 

Mutant Lines (Morning Glory Seeds)

We have 150 lines, of mainly Ipomoea nil pigmentation mutants, in storage. We also maintain and distribute wild-type I. nil, and I. purpureaI. tricolor, and other Ipomoea lines. Most of these strains have actively been used in research, therefore many of the mutant pigmentation genes have already been isolated and cloned.

Line list 1 (Japanese)
Lin2 list 2 (NBRP Site)
Phenotypes

 

EST Clones

We used a cDNA library from seedlings, and 3 cDNA libraries from flowers and buds of wild-type I. nil, also known as ‘Tokyo Kokei Standard’, to obtain EST sequences, and are now preserving over 60,000 EST clones. The DDBJ and other public database accession numbers for the 25,899 EST sequences obtained from flower and bud full-length cDNA are: BJ553078 to BJ578976. Accession numbers for the 36,336 EST sequences obtained from seedlings are: CJ736987 to CJ773322.

EST Database:http://ipomoeanil.nibb.ac.jp/

 

BAC Clones

We are preserving 89,088 BAC clones created from the ‘Tokyo Kokei Standard Line’. This covers the 800~1000 Mb Morning Glory genome 9 or 11 times. We also offer a 3D DNA pool for screening BAC DNA for PCR. With as few as 58 PCR procedures we can obtain our desired clone.

 

Expression vectors

Flower petal specific expression vectors were deposited by Dr. Shiratake of Nagoya University. For further details.

 

Distribution Procedures

For ordering information please contact Dr. Hoshino. After discussing your research goals appropriate recourses will be suggested. When the order is placed we will ask for a Material Transfer Agreement (MTA) to be filled out. Also handling & shipping remuneration will be requested (only for actual expenses).

MTA (Japanese, Wrod File)
MTA(English, Word File)

 

Resoruce Distribution Process

Resource Distribution Process

*BRP:BioResource Payment (NPO science education and training payment settlement system, Japanese only)

 

Cost Breakdown

As of April 2010 we have begun requesting remuneration of the actual costs of consumables (containers, tubes, etc.), personnel expenses (packaging, shipping, MTA processing) and postage. The actual requested resources are supplied at no cost. Please pay the costs listed on the following chart through the BRP (BioResource Payment) by credit card (credit card fees are the responsibility of the payee). We also now accept payment via bank transfer, with deferred payment as a possible option (bank transfer fees are the responsibility of the payee).

Cost Breakdown
Handling Fees (¥)
Processing Fees (¥)
Total (¥)
Seeds Handling fees per request
750
1,050
1,800
Per line
150
28
178
DNA Clones Handling fees per request
1,900
1,050
2,950
Per clone
500
95
595
Supply expense (per 10 clones)
1,300
247
1,547
Screening Kits Handling fees per request
1,100
1,050
2,150
Per kit
7,300
1,387
8,687
Shipping Materials Envelope (S) per envelope
100
5
105
Envelope (M) per envelope
100
5
105
Envelope (L) per envelope
150
8
158
Envelope (XL) per envelope
250
13
263
*Shipping Costs
Actual Cost
5.5% of Actual Cost
** 105.5% of Actual Shipping Costs

*Packages sent overseas are shipped via Express Mail Service.

**Round fractions down.

Eg: If 10 seed lines are requested there would be a ¥1800 fee for the application, a ¥1780 fee for the 10 lines, and a ¥105 fee for shipping materials, (a medium sized envelope) totaling ¥3,685, in addition to shipping costs (with a 5.5% surcharge)

Supply charge regulations (PDF File, Japanese)
Supply implementation Regulations (PDF File, Japanese))
Actual Cost reimbursement details (PDF File, Japanese)

 

Depositing Resources

We are actively collecting mutant lines of morning glories and their close relations, as well as various types of DNA clones and other genetically modified lines. We are particularly interested in collecting flower color variant mutant lines. Occasionally, new mutations occur among commercially available morning glories, or lines used in previous research are lost (such as when older garden varieties die out, or cease to be sold on the market). If you are in possession of any such new or lost seed lines, please contact us.

 

Contact: (MTA Postal Address)

Hoshino Atsushi

National Institute for Basic Biology Nishigonaka-38 Myōdaijicho Okazaki-shi, Aichi-ken, Japan, 444-8585  

Email: hoshino☆nibb.ac.jp (Please replace ☆ with @)

Phone: +81-564-55-7534