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The National Institute for Basic Biology (NIBB) is a sub-center of the National BioResource Project (NBRP) for work concerning Morning Glories. Our group works with the core institute at Kyushu University to gather, preserve, and distribute mutant lines for flower pigmentation and various DNA clones. Please contact Dr. Atsushi Hoshino for information on receiving or depositing resources. Please note our distribution is aimed only at those working within research institutes. Non academics should contact the core NBRP institute directly.

Contact: hoshino☆nibb.ac.jp (Please replace ☆ with @) 

Core Institute:Kyushu University Faculty of Science (Morning Glory Page, Japanese) 

NBRP Information Site:Morning Glory Database
NBRP Information Site:Morning Glory Project Information
NBRP Publication Database:Research Resource Circulation



Mutant Lines (Morning Glory Seeds)

We have 220 lines, mainly Ipomoea nil pigmentation mutants, in storage. We also maintain and distribute wild-type I. nil, and I. purpureaI. tricolor, and other Ipomoea lines. Most of these strains have actively been used in research. Therefore, many of the mutant pigmentation genes have already been isolated and cloned.

Line list 1 (Japanese)
Lin2 list 2 (NBRP Site)


EST Clones

We used a cDNA library from seedlings, and 3 cDNA libraries from flowers and buds of wild-type I. nil, also known as ‘Tokyo Kokei Standard’, to obtain EST sequences, and are now preserving over 60,000 EST clones. The DDBJ and other public database accession numbers for the 25,899 EST sequences obtained from flower and bud full-length cDNA are: BJ553078 to BJ578976. Accession numbers for the 36,336 EST sequences obtained from seedlings are: CJ736987 to CJ773322.

EST Database:http://ipomoeanil.nibb.ac.jp/


BAC Clones

We are preserving 105,216 BAC clones created from the ‘Tokyo Kokei Standard Line’. This covers the 800~1000 Mb Morning Glory genome 9 or 11 times. We also offer a 3D DNA pool for screening BAC DNA for PCR. With as few as 58 PCR procedures we can obtain our desired clone.


Expression vectors

Flower petal specific expression vectors were deposited by Dr. Shiratake of Nagoya University. Please click here for further details.


Distribution Procedures

Please contact Dr. Hoshino regarding how to place orders. After discussing your research goals appropriate recourses will be suggested. Once the order is placed we will ask for a Material Transfer Agreement (MTA) to be filled out. Also handling & shipping remuneration will be requested (only for actual expenses).

MTA (Japanese, Word File)
MTA(English, Word File)


Resoruce Distribution Process

Resource Distribution Process

*BRP:BioResource Payment (NPO science education and training payment settlement system, Japanese only)


Cost Breakdown

As of April 2010 we have begun requesting remuneration of the actual costs of consumables (containers, tubes, etc.), personnel expenses (packaging, shipping, MTA processing) and postage. The actual requested resources are supplied at no cost. Please pay the costs listed on the following chart through the BRP (BioResource Payment) by credit card (credit card fees will be borne by the payee). We also now accept payment via bank transfer, with deferred payment as a possible option (bank transfer fees will be borne by the payee).

Cost Breakdown
Handling Fees (¥)
Processing Fees (¥)
Total (¥)
Seeds Handling fees per request 750 1,155 1,905
Per line 150 31 181
DNA Clones Handling fees per request 1,900 1,155 3,055
Per clone 500 104 604
Supply expense (per 10 clones) 1,400 292 1,692
Screening Kits Handling fees per request 1,100 1,155 2,255
Per kit 7,500 1,567 9,067
Shipping Materials Envelope (S) per envelope 100 5 105
Envelope (M) per envelope 100 5 105
Envelope (L) per envelope 150 8 158
Envelope (XL) per envelope 300 16 316
*Shipping Costs
Actual Cost
5.5% of Actual Cost
** 105.5% of Actual Shipping Costs

*Packages sent overseas are shipped via Express Mail Service.

**Round fractions down.

Example: If 10 seed lines were to be requested, there would be a ¥1,905 application fee, a ¥1,810 fee for the 10 lines, and a ¥105 fee for shipping materials, (a medium sized envelope) totaling ¥3,820, in addition to shipping costs (with a 5.5% surcharge)

Supply charge regulations (PDF File, Japanese)
Supply implementation Regulations (PDF File, Japanese))
Actual Cost reimbursement details (PDF File, Japanese)


Depositing Resources

We are actively collecting mutant lines of morning glories and their close relations, as well as various types of DNA clones and other genetically modified lines. We are particularly interested in collecting flower color variant mutant lines. Occasionally, new mutations occur among commercially available morning glories, or lines used in previous research are lost (such as when older garden varieties die out, or cease to be sold on the market). If you are in possession of any such new or lost seed lines, please contact us.


Contact: (MTA Postal Address)

Hoshino Atsushi

National Institute for Basic Biology Nishigonaka-38 Myōdaijicho Okazaki-shi, Aichi-ken, Japan, 444-8585  

Email: hoshino☆nibb.ac.jp (Please replace ☆ with @)

Phone: +81-564-55-7534