Genome Editing and Imaging
of Fish and Amphibians

Thu, September 20^th, 2018
11:00                   Registration opens
13:30                   Opening Remarks
14:00 – 15:00      Orientation, Guidance, and Animal welfare lecture/Dr. Kiyoshi Naruse
15:00 – 18:00      Genome Editing lecture and experiment (Medaka)/Dr. Satoshi Ansai
18:00 – 20:00      Welcome Party


Fri, September 21^st, 2018
  9:00 – 12:00      Microinjection lecture and experiment (Medaka)/Dr. Satoshi Ansai
13:30 – 17:00      Construction of Guide RNA/Dr. Satoshi Ansai
17:00 – 18:00      Seminar presented by Dr. Pung-Pung Hwang (Academia Sinica, Taiwan)
18:00 – 20:00      Social Hour (Presentation from participants and discussion with lecturers)
 

 Sat, September 22^nd, 2018
  9:00 – 12:00      Microinjection experiment (Medaka)
13:30 – 15:30      Microinjection experiment (Medaka)
15:30 – 17:00      Husbandry lecture/Dr. Kiyoshi Naruse
17:00 – 19:00      Seminar presented by Dr. Hitoshi Yokoyama (Hirosaki University)
                            Seminar presented by Dr. Satoshi Ansai (NIBB)

  
Sun, September 23^rd, 2018
No set program
 
 
Mon, September 24^th, 2018
No set program
 
 
Tue, September 25^th, 2018
  9:00 – 12:00      Xenopus lecture/Dr. Hajime Ogino
                            Genome Editing lecture (Xenopus)/Dr. Ken-ichi T. Suzuki
13:30 – 17:00      Microinjection experiment (Xenopus)/Drs. Hajime Ogino and Ken-ichi T. Suzuki
17:00 – 18:00      Seminar presented by Dr. Takeshi Igawa (Hiroshima University)
18:00 – 20:00      Social Hour (Presentation from participants and discussion with lecturers)
 
 
Wed, September 26^th, 2018
  8:40 – 9:00        Xenopus egg selection
  9:00 – 12:30      IR-LEGO lecture/Dr. Yasuhiro Kamei
                            Light-sheet LSM lecture/Dr. Shigenori Nonaka
14:00 – 17:30      IR-LEGO operation (Medaka and Xenopus)
                            Observation using Light-sheet LSM (Medaka and Xenopus)
17:30 – 19:30      Seminar presented by Dr. Haruki Ochi (Yamagata University)
                            Seminar presented by Dr. Toshinori Hayashi (Tottori University / iNewt)
 
 
Thu, September 27^th, 2018
  9:00 – 12:00      Lecture about Regeneration Study using Amphibians/Dr. Toshinori Hayashi
                            Regeneration Operation using Xenopus/Dr. Aiko Kawasumi
13:30 – 17:00      Regeneration Operation using Iberian Ribbed Newt/Dr. Toshinori Hayashi
                            Observation of IR-LEGO treated embryos
17:00 – 19:00      Seminar presented by Dr. Lazaro Centanin (Heidelberg University, Germany)
                            Seminar presented by Dr. Takuji Sugiura (IMP, Austria)

 
Fri, September 28^th, 2018
  9:00 – 12:00      Lecture and experiment about Imaging using LSM/Dr. Shigenori Nonaka
13:30 – 17:00      Cryopreservation lecture and experiment/Dr. Kiyoshi Naruse
17:00 – 19:00      Seminar presented by Dr. Shinya Komoto (OIST)
                            Seminar presented by Dr. Asano Ishikawa (National Institute of Genetics)
18:00– 20:00       Farewell Party (Group discussion about future research and feedback on the course)
 

Sat, September 29^th, 2018
  8:40 – 9:00        Observation of CRSPR/Cas9 Injected Xenopus
  9:00 – 12:00      Lecture and experiment about Behavior Assay/Dr. Shoji Fukamachi
13:30 – 15:00      Data Analysis of Behavior Assay/Dr. Yoriko Ando
15:30                   Closing Ceremony