15. Available plasmids (You cannot see maps of plasmids with Internet Explorer. Please use other blowers.)

Plasmids are available upon request to Mitsuyasu Hasebe (mhasebe@nibb.ac.jp). Most plasmids are distributed before publication as a volunteer for convenience of Physco friends without any funds.

We found that 7133 promoter we has been used has a sequence of 7113 promoter. The original plasmid we obtained from Drs. Mitsuhara and Ohashi has the 7113 plasmid with a label of 7133 on the tube. Therefore all plasmids we used in my laboratory as 7133 should be 7113.

1. Plasmids for gene disruption

No	Name	marker	comment
1	pTN3	nptII	G418 resistant cassette
2	pTN80	nptII	Modified nptII cassette
3	pTN81	nptII	Modified nptII cassette
4	pTN82	nptII	Floxed modified nptII cassette
5	pTN182	nptII	Floxed modified nptII cassette, MCS variant
6	pTN86	aph4	Floxed modified aph4 cassette
7	pTN186	aph4	Floxed modified aph4 cassette, MCS varinat
8	p35S-Zeo	ble	Zeocin resistant cassette
9	p35S-loxP-Zeo	zeocin	Floxed modified zeocin resistant cassette
10	p35S-loxP-BSD	Blastcidin S	Floxed modified blastcidin S resistant cassette

2. Plasmids for a reporter gene assay

No	Name	reporter	marker	comment
1	pTN83	uidA	nptII	Modified nptII cassette: to fuse GUS to a C terminal
2	pTN84	uidA	nptII	Floxed modified nptII cassette: to fuse GUS to a C terminal
3	pTN85	uidA	nptII	GUS (M1L), Floxed modified nptII cassette: to fuse GUS to a C terminal
4	pGFPmutNPTII	gfp	nptII	GFP(M1L): to fuse GFP to a C terminal
5	pYHG2	gfp	aph4	GFP(M1L): to fuse GFP to a C terminal
6	plinkerYHG2	gfp	aph4	GFP(M1L), linker sequence just before GFP;　 to fuse GFP to a C terminal
7	pHIZ2	gfp	Ble	GFP(M1L): to fuse GFP to a C terminal
8	pHIZ3	gfp	ble	to fuse GFP to a N terminal
9	pCTRN-NPTII2	citrine	nptII	To fuse citrine to either terminal

3. Plasmids for overexpression analysis

No	Name	Promoter	marker	Target site	Comment & references
1	pPpMADS2-7113	E7113	nptII	PpMAD2
2	pPpMADS2-7113 rev	E7113	nptII	PpMAD2
3	pPphb7-7113	E7113	nptII	Pphb7
4	pPphb7-7113 rev	E7113	nptII	Pphb7
5	pTFH9119	Actin	nptII	PpMAD2
6	pPpMADS2-Actin rev	Actin	nptII	PpMAD2
7	pTN90	Actin	nptII	Pphb7
8	pTFH15.3	Actin	nptII	Pphb7
9	pZAG1	Actin	ble	213 locus	GFP-fusion
10	pMAK1	7113	ble	213 locus
11	pCMAK1	7113	ble	213 locus
12	pTFH38.9N	CaMV35S	ble	213 locus	mRFP-fusion
13	pTFH38.9C	CaMV35S	ble	213 locus	mRFP-fusion
14	pKS2	Actin	nptII		GFP-fusion
15	pActN-GFP	Actin	nptII		GFP-fusion
16	pTFH22.4	Actin	nptII GFP
17	pTKM1	Actin	None		Transient expression