pPphb7 7133

Designed and constructed by Rumiko Kofuji

Primers:

PMADS2-5: 5’- AGT GGA TTG TAG GGA TGG TCC AGC GAG C-3’

PMADS2-3: 5’- TGA AAA ATC TCC GGC ACT CCA CGC CAA C-3’

PHB9-52: 5’- TAG CGG CCG CGG AAA GGG GAG GGA AGG GTG TAA-3’

PHB9-32: 5’- TAG CGG CCG CTC AGG GAC GCA CAA CAG CGA CAA-3’

The following primers are located outside the genomic region of the PpHB9 platform.　 You can check whether this vector was targeted into the PpHB9 locus or not by PCR with these primers

PpHB9-51: 5’- GTG TCG AGC GCG TCG GCA AGA G-3’

PpHB9-31: 5’- AAA GAG GTG CCA CTT TAT TCC AT-3’