pPpMAD2 7133
Designed and
constructed by Rumiko Kofuji
Primers:
PMADS2-5:
5’- AGT GGA TTG TAG GGA TGG TCC AGC GAG C-3’
PMADS2-3:
5’- TGA AAA ATC TCC GGC ACT CCA CGC CAA C-3’
PHB9-52:
5’- TAG CGG CCG CGG AAA GGG GAG GGA AGG GTG TAA-3’
PHB9-32:
5’- TAG CGG CCG CTC AGG GAC GCA CAA CAG CGA CAA-3’
The
following primers are located outside the genomic region of the PpHB9
platform. You can check whether this
vector was targeted into the PpHB9 locus or not by PCR with these primers
PpHB9-51:
5’- GTG TCG AGC GCG TCG GCA AGA G-3’
PpHB9-31:
5’- AAA GAG GTG CCA CTT TAT TCC AT-3’