pTN90

Designed and constructed by Tomoaki Nishiyama

Sequence

>pTN90

ggaaaggggagggaagggtgtaaggaggcccacgggcagggtcaaggtgtccaatgcacctgcaagatcaggaagcttgaagtagatcagggaacaaacgatggtagtccctagtttacccgccttcggaggacagaacgccatgctcagacgcaacattgacaacaacaccgacactctgatttctctgcttcaagggtcctgctcccctcgcgtgagcatgcaacaaggtgtgcattatatgctttacactcgatcctacactgggcatacgtgattagatttgcaccatgcgaaggtgcgttggaaatgcgtgcaaggtgcaatgcaggttgcttagccactcttatttttgaagacacttgtttccgagttggtagagcaaagtttctgctaagagagcttgtcgcacgatagccttcagaaaatcggcctagaaatgagcttcgtcaagggaacaactcaatgttgaggacatagccagtccgttggtgatatgctcaactgttgtctgtgccttactcttcttctgcaaaccgctccttgttgaagggcctcgcttgttctacatggtaaaggtacataaatgattccggttgaaaagttcatgtaggtaggtggtgtgaatgtttggctcgctagatatgttgctagttttttcgtcggacgatgttgtcatttttgttctccttcgtctggttacgctgagcatttcgcagtgttgaccggatatgatacacgagcattgcaagaaaagagaattgaagctttttcgatgtcaccttgtgtgaagtgacgtgcggttgattgaacttcgggctgcattttggcccacttgtctctcggtccatctcttcgcataagacttaaagacgcccaacattttaagattaaggattcgtggaaccgtcactatgaatgtgaaaaatgtattcgcatgtgcgacaaatatctttcaccggtattccttttggcactccgctcggcacaacgatttcgcagagcgattctctattagaccttgtattctctctacagaaaacacttcaagcagaatcactaaaaatcccttgatctggtggacaatattatctttgttgtcctcgcctgccatctgaaggagatgctgttcagtcctctcttgttggattttgagttgaacaagtatctaatcgaatctgcaggatatgttgcagtgccgcgttcatcggagagtctcgaaaacatgatgggggcttgtgggcaaaaactgccttacttttcgtcatttgatgggccgagtgtagaagagcaagaggatgtcgacgaaggtatcgacgaattcgcacaccacAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATGTGAGTTAGCTCACTCATTAGGCACCCCAGGCTTTACACTTTATGCTTCCGGCTCGTATGTTGTGTGGAATTGTGAGCGGATAACAATTTCACACAGGAAACAGCTATGACCATGATTACGAATTCGAGCTCGGTACCCCTCAAGCTTCGAGGTCATTCATATGCTTGAGAAGAGAGTCGGGATAGTCCAAAATAAAACAAAGGTAAGATTACCTGGTCAAAAGTGAAAACATCAGTTAAAAGGTGGTATAAAGTAAAATATCGGTAATAAAAGGTGGCCCAAAGTGAAATTTACTCTTTTCTACTATTATAAAAATTGAGGATGTTTTTGTCGGTACTTTGATACGTCATTTTTGTATGAATTGGTTTTTAAGTTTATTCGCTTTTGGAAATGCATATCTGTATTTGAGTCGGGTTTTAAGTTCGTTTGCTTTTGTAAATACAGAGGGATTTGTATAAGAAATATCTTTAAAAAAACCCATATGCTAATTTGACATAATTTTTGAGAAAAATATATATTCAGGCGAATTCTCACAATGAACAATAATAAGATTAAAATAGCTTTCCCCCGTTGCAGCGCATGGGTATTTTTTCTAGTAAAAATAAAAGATAAACTTAGACTCAAAACATTTACAAAAACAACCCCTAAAGTTCCTAAAGCCCAAAGTGCTATCCACGATCCATAGCAAGCCCAGCCCAACCCAACCCAACCCAACCCACCCCAGTCCAGCCAACTGGACAATAGTCTCCACACCCCCCCACTATCACCGTGAGTTGTCCGCACGCACCGCACGTCTCGCAGCCAAAAAAAAAAAAGAAAGAAAAAAAAGAAAAAGAAAAAACAGCAGGTGGGTCCGGGTCGTGGGGGCCGGAAACGCGAGGAGGATCGCGAGCCAGCGACnAGGCCGGCCCTCCCTCCGCTTCCAAAGAAACGCCCCCCATCGCCACTATATACATnCCCCCCCCTCTCCTCCCATCCCCCCAACCCcTACCACCACCACCACCACCACCTCCACCTCCTCCCCCCTCGCTGCCGGACGACGAGCTCCTCCCCCCTCCCCCTCCGCCGCCGCCGCGCCGGTAACCACCCCGCCCCTCTCCTCTTTCTTTCTCCGTTTTTTTTTTCCGTCTCGGTCTCGATCTTTGGCCTTGGTAGTTTGGGTGGGCGAGAGGCGGCTTCGTGCGCGCCCAGATCGGTGCGCGGGAGGGGCGGGATCTCGCGGCTGnGGsTCTCGCCGGCGTGGATCCGGCCCGGATCTCGCGGGGAATGGGGCTCTCGGATGTAGATCTGCGATCCGCCGTTGTTGGGGGAGATGATGGGGGGTTTAAAATTTCCgCCATGcTAAaCAAGATCAGGAAGAGGGGAAAAGGGCACTATGGTTTATATTTTTATATATTTCTGCTGCTTCGTCAGGCTTAGATGTGCTAGATCTTTCTTTCTTCTTTTTGTGGGTAGAATTTGAATCCCTCAGCATTGTTCATCGGTAGTTTTTCTTTTCATGATTTGTGACAAATGCAGCCTCGTGCGGAGCTTTTTTGTAGGTAGAAGATATCAGCTTTCGTCCGTATCATCGGTTTCGACAACGTTCGTCAAGTTCAATGCATCAGTTTCATTGCCCACACACCAGAATCCTACTAAGTTTGAGTATTATGGCATTGGAAAAGCTGTTTTCTTCTATCATTTGTTCTGCTTGTAATTTACTGTGTTCTTTCAGTTTTTGTTTTCGGACATCAAAATGCAAATGGATGGATAAGAGTTAATAAATGATATGGTCCTTTTGTTCATTCTCAAATTATTATTATCTGTTGTTTTTACTTTAATGGGTTGAATTTAAGTAAGAAAGGAACTAACAGTGTGATATTAAGGTGCAATGTTAGACATATAAAACAGTCTTTCACCTCTCTTTGGTTATGTCTTGAATTGGTTTGTTTCTTCACTTATCTGTGTAATCAAGTTTACTATGAGTCTATGATCAAGTAATTATGCAATCAAGTTAAGTACAGtATAGGCTTTTTGTGTCGACATTTAAATATCGAATTCCTGCAGCCCCCCTACTCCAAAAATGTCAAAGATACAGTCTCAGAAGACCAAAGGGCTATTGACACTTTTCAACAAAGGGTATATAATCGGGAAGACTCCTCGGATTCCATTGCCCAGCTATCTGTCaCTTCATCGAAAGGACAGTAGAAAAGGAAGGTGGCTCCTACAAATGCCATCATTGCGATAAAGGAAAGGCTATCATTCAAGATGCCTCTGCCGACAGTGGTCCCAAAGATGGACCCCCACCCACGAGGAGCATCGTGGAAAAAGAAGACGTTCCAACCACGTCTTCAAAGCAAGTGGATTGATGTGACATCTCCACTGACGTAAGGGATGACGCACAATCCCACTATCCTTCGCAAGACCCTTCCTCTATATAAGGAAGTTCATTTCATTTGGAGAGGACAGCCCAAGCAAGGAGGTGAGCATGATTGAACAAGACGGATTGCACGCAgGTTCTCCGGCCGCTTGGGTGGAGAGGCTATTCGGCTATGACTGGGCACAACAGACAATCGGCTGCTCTGATGCCGCCGTGTTCCGGCTGTCAGCGCAGGGGCGCCCGGTTCTTTTTGTCAAGACCGACCTGTCCGGTGCCCTGAATGAACTCCAAGACGAGGCAGCGCGGCTATCGTGGCTGGCCACGACGGGCGTTCCTTGCGCAgCTGTGCTCGACGTTGTCACTGAAgCGGGAAGGGACTGGCTGCTATTGGGCGAAGTGCCGGGGCAGGAtCTCCTGTCATCTCACCTTGCtCCTGCCGAGAAAGTATCCATCATGGCTGATGCAATGCGGCGGCTGCATACGCTTGATCCGGCTACCTGCCCATTCGACCACCAAGCGAAACATCGCATCGAGCGAGCACGTACTCGGATGGAAGCCGGTCTTGTCGATCAGGATGATCTGGACGAAGAGCATCAGGGGCTCGCGCCAGCCGAACTGTTCGCCAGGCTCAAGGCGCGGATGCCCGACGGCGAGGATCTCGTCGTGACCCATGGCGATGCCTGCTTGCCGAATATCATGGTGGAAAATGGCCGCTTTTCTGGATTCATCGACTGTGGCCGGCTGGGTGTGGCGGACCGCTATCAGGACATAGCGTTGGCTACCCGTGATATTGCTGAAGAGCTTGGCGGCGAATGGGCTGACCGCTTCCTCGTGCTTTACGGTATCGCCGCTCCCGATTCGCAGCGCATCGCCTTCTATCGCCTTCTTGACGAGTTCTTCTGAGCGGGACTCTGGGGTTCGGACGGTACGCTGAAATCACCAGTCTCTCTCTACAAATCTATCTCTCTCTATTTTCTCnATAAATAATGTGTGAGTAGTTTCCCGATAAGGGAAATTAGGGTTCTTATAGGGTTTCGCTCATGTGTTGAGCATATAAGAAACCCTTAGTATGTATTTGTATTTGTAAAATACTTCTATCAATAAAATTTCTAATTCCTAAAACCAAAATCCAGTACTAAAATCCAGATCTCCTAAAGTCCCTATAGATCTTTGTCGTGAATATAAACCAGACACGAGACGACTAAACCTGGAGCCCAGACGCCGTTCGAAGCTAGAAGTACCGCTTAGGCAGGAGGCCGTTAGGGAAAAGATGCTAAGGCAGGGTTGGTTACGTTATCGGGGATCCTCTAGAGTCGACCTGCAGGCATGCAAGCTTGGCACTGGCCGTCGTTTTACAgtggagaaaaagaggagattgtcattagaacaagtgcgatcattagaacggaattttgaagtggaaaacaagcttgagcccgagaggaaaatgcaactagctaaggagcttggactgcaacctcgtcaagtggcggtgtggttccagaatagacgggcaaggtggaaaaccaaacagctcgagcgcgactacgagaccctgaagaaagcctacgacaggcttaaagcagacttcgaagccgttactctagacacaagtgctcttaaagctgaggtacttagcatcttaatcctttccgcttgccttttcaaatttgagctcaaggagtgccaagtctcttgtttttgctgtgttcaccatacattaccaaaacctaacctcccatttcgctctgttttgcaggtgagtcgcctcaagggaatctctaatgacgacgtcaagcccgccgaattcgttcagggcaagtgtgacacaacgagtcaccctgcctcccctgcgcaatcggagaggtccgacattgtgtcatcgaggaatcgcacaactcctaccatacatgtggatcccgtggcacccgaggaagccggcgctcacttaaccatgagctcggatagcaattccagcgaggtcatggacgctgatagccctcgcacgagccacaccagcgctagtaggagcactttgtccacaagtgtggtgcagcctgacgagggcctgggagtggcccagtacccccacttttctcccgaaaacttcgtgggtcccaatatgccagagatttgcgctgatcagtcacttgcatctcaagtgaagctggaagagatccacagcttcaatcccgaccaaaccttcctgctcttgcccaactggtgggattgggcttgattcgtttcttcatctgtacccatacactttttccttgaatccaagttgaattcactttaggcagtgttttttcacgatgtaccacttgttattcttccaccatgtgcaatccaacgtcaaccaaagttgcaccatcggcgatcattggtgacgatgtcgagcatcgatcgtcaccatgccagtcggcgcttgtgcctttttgaacagcagcaggaaggcaacagaaaactctgggaaaagcaaccaggagcttcgaagttaggtgctgcaaccttgctagatatatgatcgaatcttgaggaattatgtagttaagtagttggacttatgtaaaaagtcagagtagggacacatagcccctttggaccagagctagaaccgaaacacactaatcggattactacaacttcaattcacttgccttggtgacacagtgatggcgatggtccgtgcatctgcagtattgttgtcggccgtgtaattgtacatatgcgaataagcattggttctcaaggtaggaaaggaggaaaaaatctttcgttgtcgctgttgtgcgtccctgagcggccgc

Lowercase letters: 5’or 3’ PpHB9 region

　　　 : Sse9793I

　　　 : NotI

　　　 : EcoRV

　　　 : KpnI

You can excise a region from Actin promoter (Pact) to the modified nptII cassette from this plasmid with KpnI and Sse9793I.