研究業績

  1. Chikami, Y., Okuno, M., Toyoda, A., Itoh, T. and Niimi, T. (2022) Evolutionary history of sexual differentiation mechanism in insects. Mol. Biol. Evol., 39, in press.  DOI: 10.1093/molbev/msac145
  2. Sakura, K., Morita, S. and Niimi, T. (2022) RNA interference method for gene function analysis in the Japanese rhinoceros beetle Trypoxylus dichotomus. Bio-protoc., 12, e4396.  DOI: 10.21769/BioProtoc.4396
  3. Ohde, T., Mito, T. and Niimi, T. (2022) A hemimetabolous wing development suggests the wing origin from lateral tergum of a wingless ancestor. Nat. Commun., 13, 979. DOI: 10.1038/s41467-022-28624-x.
  4. Morita, S., Sakura, K., Gotoh, H., Emlen, D. J., Niimi, T. (2022) Recent advances in understanding horn formation in the Japanese rhinoceros beetle Trypoxylus dichotomus using next generation sequencing technology. Curr. Opin. Insect Sci., in press. DOI: 10.1016/j.cois.2022.100901
  5. Shigenobu, S., Hayashi, Y., Watanabe, D. Tokuda, G., Hojo, M. Y., Toga, K., Saiki, R., Yaguchi, H., Masuoka, Y., Suzuki, R., Suzuki, S. Kimura, M. Matsunami, M., Sugime, Y., Oguchi, K., Niimi, T., Gotoh, H., Hojo, M. K., Miyazaki, S., Toyoda, A., Miura, T. and Maekawa, K. (2022) Genomic and transcriptomic analyses of the subterranean termite Reticulitermes speratus: Gene duplication facilitates social evolution. Proc. Natl. Acad. Sci. USA., 119, e2110361119. DOI: 10.1073/pnas.2110361119
  6. Miyazaki, S., Fujiwara, K., Kai, K., Masuoka, Y., Gotoh, H., Niimi, T., Hayashi, Y., Shigenobu, S. and Maekawa, K. (2021) Evolutionary transition of doublesex regulation from sex-specific splicing to male-specific transcription in termites. Sci. Rep.,11, 15992. DOI: 10.1038/s41598-021-95423-7.
  7. Hashiro, S., Chikami, Y., Kawaguchi, H., Krylov, A. A., Niimi, T. and Yasueda, H. (2021) Efficient production of long double-stranded RNAs applicable to agricultural pest control by Corynebacterium glutamicum equipped with coliphage T7-expression system. Appl. Microbiol. Biotechnol., 105, 4987-5000. DOI: 10.1007/s00253-021-11324-9
  8. Chikami, Y., Kawaguchi, H., Suzuki, T., Yoshioka, H., Sato, Y., Yaginuma, T. and Niimi, T. (2021) Oral RNAi of diap1 results in rapid reduction of damage to potatoes in Henosepilachna vigintioctopunctata. J. Pest Sci., 94, 505–515. DOI 10.1007/s10340-020-01276-w
  9. Niimi, T. and Ando, T. (2021) Evo-devo of wing colour patterns in beetles. Curr. Opn. Genet. Dev., 69, 97-102. DOI: doi.org/10.1016/j.gde.2021.02.007
  10. Adachi, H., Matsuda, K., Niimi, T., Kondo, S., and Gotoh, H. (2020). Genetical control of 2D pattern and depth of the primordial furrow that prefigures 3D shape of the rhinoceros beetle horn. Sci. Rep. 10. DOI: 10.1038/s41598-020-75709-y
  11. Sakai, H., Konagaya, T.,Takemura, Y.,Sahara, K. and Niimi, T. (2020) Double-copulated introduction of ejaculate with dominant larval phenotype to maintain Bombyx mori mutant with dysfunctional apyrene sperm. J. Insect Biotechnol. Sericol., 89, 39-43.DOI: doi.org/10.11416/jibs.89.2_039
    (2020年度 日本蚕糸学会進歩賞 奨励賞)
  12. 森田慎一・新美輝幸(2020) カブトムシの角発生メカニズム. 蚕糸昆虫バイオテック, 89, 145-151.
  13. 新美輝幸(2020) 特集:「武器形質研究の最前線」にあたって. 蚕糸昆虫バイオテック, 89, 125-131.
  14. 大出高弘・新美輝幸 (2020) 2.5.7昆虫の上陸と翅の獲得「動物の事典」(末光隆志編),朝倉書店, 東京.
  15. 酒井弘貴・小長谷達郎・新美輝幸 (2020) カイコにおける二重交尾とその応用. 昆虫DNA研究会ニュースレター33,41-44.
  16. 安藤俊哉・新美輝幸 (2020) ナミテントウの斑紋形成メカニズム. 昆虫と自然, 55, 5-8.
  17. 新美輝幸 (2020) 総論テントウムシ研究の最前線. 昆虫と自然, 55, 2-4.
  18. Hashiro, S., Mitsuhashi, M., Chikami, Y., Kawaguchi, H., Niimi, T. and Yasueda, H. (2019) Construction of Corynebacterium glutamicum cells as containers encapsulating dsRNA overexpressed for agricultural pest control. Appl. Microbiol. Biotechnol., 103, 8485-8496. DOI: 10.1007/s00253-019-10113-9
  19. Sakai, H., Oshima, H., Yuri, K., Gotoh, H., Daimon, T., Yaginuma, T., Sahara, K. and Niimi, T. (2019) Dimorphic sperm formation by Sex-lethal. Proc. Natl. Acad. Sci. USA., 116, 10412-10417.DOI: 10.1073/pnas.1820101116
  20. Morita, S., Ando, T., Maeno, A., Mizutani, T., Mase, M., Shigenobu, S. and Niimi, T. (2019) Precise staging of beetle horn formation in Trypoxylus dichotomus reveals the pleiotropic roles of doublesex depending on the spatiotemporal developmental contexts. PLOS Genet., 15, e1008063. DOI: 10.1371/journal.pgen.1008063
  21. 新美輝幸・安藤俊哉・森田慎一 (2019) RNAi法で探る非モデル昆虫の形作りの仕組み. 日本農薬学会誌, 44, 219-225.
  22. 酒井弘貴・新美輝幸(2019)無核精子の形成に関与する遺伝子の特定.昆虫DNA研究会ニュースレター31,16-18.
  23. 森田慎一・新美輝幸 (2019) カブトムシの角の形成に性差ができる時期を特定!. 化学, 74, 73.
  24. 千頭康彦・森田慎一・新美輝幸 (2019) 鞘翅目における性決定と性分化. 昆虫と自然, 54, 15-18.
  25. 新美輝幸 (2019) 総論昆虫の性決定. 昆虫と自然, 54, 2-3.
  26. 酒井弘貴・新美輝幸・冨田秀一郎 (2019) 実験23 カイコのホメオティック変異体の観察.「カイコの実験単」(日本蚕糸学会監修), 株式会社エヌ・ティー・エス, 東京. P204-209.
  27. Ando, T. and Niimi, T. (2019) Development and evolution of color patterns in ladybird beetles: A case study in Harmonia axyridis. Dev. Growth Differ., 61, 73-84. DOI: 10.1111/dgd.12592
  28. Zinna, R., Emlen, D., Lavine, L. C., Johns, A., Gotoh, H., Niimi, T. and Dworkin, I. (2018) Sexual dimorphism and heightened conditional expression in a sexually selected weapon in the Asian rhinoceros beetle. Mol. Ecol., 27, 5049-5072. DOI: 10.1111/mec.14907
  29. Sota, T., Sugawara, H., Fujisawa, T., Fujimaki, K. and Niimi, T. (2018) Knockdown of rotund gene through larval RNA interference affects genital and elytral morphology in the ground beetle Carabus maiyasanus (Coleoptera: Carabidae). Entomol. Sci., 21, 469-474. DOI: 10.1111/ens.12330
  30. Ohde, T., Morita, S., Shigenobu, S., Morita, J., Mizutani, T., Gotoh, H., Zinna, R. A., Nakata, M., Ito, Y., Wada, K., Kitano, Y., Yuzaki, K., Toga, K., Mase, M., Kadota, K., Rushe, J., Lavine, L. C., Emlen, D. J. and Niimi, T. (2018) Rhinoceros beetle horn development reveals deep parallels with dung beetles. PLOS Genet., 14, e1007651. DOI: 10.1371/journal.pgen.1007651
  31. Ando, T., Matsuda, T., Goto, K., Hara, K., Ito, A., Hirata, J., Yatomi, J., Kajitani, R., Okuno, M., Yamaguchi, K., Kobayashi, M., Takano, T., Minakuchi, Y., Seki, M., Suzuki, Y., Yano, K., Itoh, T., Shigenobu, S., Toyoda, A. and Niimi, T. (2018) Repeated inversions within a pannier intron drive diversification of intraspecific colour patterns of ladybird beetles. Nat. Commun., 9, 3843.DOI: 10.1038/s41467-018-06116-1
    (Recommended by F1000)
  32. Ohde, T., Takehana, Y., Shiotsuki, T. and Niimi, T. (2018) CRISPR/Cas9-based heritable targeted mutagenesis in Thermobia domestica: A genetic tool in an apterygote development model of wing evolution. Arthropod Struct. Dev., 47, 362-369. DOI: 10.1016/j.asd.2018.06.003
  33. Kawaguchi, H. and Niimi, T. (2018) A method for cryopreservation of ovaries of the ladybird beetle, Harmonia axyridis. J. Insect Biotechnol. Sericol., 87, 35-44. DOI: https://doi.org/10.11416/jibs.87.2_035
  34. Adachi, H., Matsuda, K., Niimi, T., Inoue, Y., Kondo, S. and Gotoh, H. (2018) Anisotropy of cell division and epithelial sheet bending via apical constriction shape the complex folding pattern of beetle horn primordia. Mech. Dev., 152, 32-37. DOI:  10.1016/j.mod.2018.06.003
  35. Tsuji, T., Gotoh, H., Morita, S. Hirata, J., Minakuchi, Y., Yaginuma, T., Toyoda, A. and Niimi, T. (2018) Molecular characterization of eye pigmentation-related ABC transporter genes in the ladybird beetle Harmonia axyridis reveals striking gene duplication of the white gene. Zool. Sci., 35, 260-267. DOI: 10.2108/zs170166
  36. Hust, J., Lavine, M. D., Worthington, A. M., Zinna, R., Gotoh, H., Niimi, T. and Lavine, L. (2018) The Fat-Dachsous signaling pathway regulates growth of horns in Trypoxylus dichotomus, but does not affect horn allometry. J. Insect Physiol., 105, 85-94. DOI: 10.1016/j.jinsphys.2018.01.006
  37. Zinna, R., Gotoh, H., Kojima, T. and Niimi, T. (2018) Recent advances in understanding the mechanisms of sexually dimorphic plasticity: Insights from beetle weapons and future directions. Curr. Opi. Insect Sci., 25, 35–41. DOI: 10.1016/j.cois.2017.11.009
  38. 新美輝幸 (2018) 第1章 男になるか女になるか.「昆虫たちの不思議な性の世界」(大場裕一編), 一色出版, 東京. P27-79.
  39. 新美輝幸 (2018) 第2章 男らしさ女らしさ.「昆虫たちの不思議な性の世界」(大場裕一編), 一色出版, 東京. P85-130.
  40. Matsuda, K., Gotoh, H., Tajika, Y., Sushida, T., Aonuma, H., Niimi, T., Akiyama, M., Inoue, Y. and Kondo, S. (2017) Complex furrows in a 2D epithelial sheet code the 3D structure of a beetle horn. Sci. Rep., 7, 13939. DOI: 10.1038/s41598-017-14170-w
  41. 畠山正統・千頭康彦・新美輝幸 (2017) ハチ目(膜翅目)とコウチュウ目(鞘翅目)におけるゲノム編集研究. 蚕糸・昆虫バイオテック, 86, 115-124.
  42. 森田慎一・新美輝幸 (2017) 武器形質を作る遺伝子.「クワガタムシ、カブトムシ学の最新研究」(長谷川道明編), 豊橋自然史博物館, 愛知. P21-24.
  43. Ozawa, T., Mizuhara, T., Arata, M., Shimada, M., Niimi, T., Okada, K., Okada, Y. and Ohta, K. (2016) Histone deacetylases control module-specific phenotypic plasticity in beetle weapons. Proc. Natl. Acad. Sci. USA., 113, 15042-15047.DOI: 10.1073/pnas.1615688114
  44. Zinna, R., Gotoh, H., Brent, C. S., Dolezal, A., Kraus, A., Niimi, T., Emlen, D. and Lavine, L. C. (2016) Endocrine control of exaggerated trait growth in rhinoceros beetles. Integr. Comp. Biol., 56, 247-259.doi: DOI: 10.1093/icb/icw042
  45. Gotoh, H., Ishiguro, M., Nishikawa, H., Morita, S., Okada, K., Miyatake, T., Yaginuma T. and Niimi, T. (2016) Molecular cloning and functional characterization of the sex-determination gene doublesex in the sexually dimorphic broad-horned beetle Gnatocerus cornutus (Coleoptera, Tenebrionidae). Sci. Rep., 6, 29337.DOI: 10.1038/srep29337
  46. Gotoh, H., Zinna, R. A., Warren, I., DeNieu, M., Niimi, T., Dworkin, I., Emlen, D. J., Miura, T. and Lavine, L. C. (2016) Identification and functional analyses of sex determination genes in the sexually dimorphic stag beetle Cyclommatus metallifer. BMC Genomics, 17, 250. DOI: 10.1186/s12864-016-2522-8
  47. 大出高弘・新美輝幸 (2016) 昆虫の翅の起源はどこか?. 昆虫と自然, 51, 4-7.
  48. 新美輝幸 (2016) 総論昆虫が独自に獲得した新奇形質. 昆虫と自然, 51, 2-3.
  49. 間瀬睦月・大出高弘・新美輝幸 (2016) 性決定遺伝子で探るカブトムシの角形成メカニズム. 化学と生物, 54, 586-590.
  50. Hatakeyama, M., Yatomi, J., Sumitani, M., Takasu, Y., Sekiné, K., Niimi, T. and Sezutsu, H. (2015) Knockout of a transgene by transcription activator-like effector nucleases (TALENs) in the sawfly, Athalia rosae (Hymenoptera) and the ladybird beetle, Harmonia axyridis (Coleoptera). Insect Mol. Biol.,25,24-31.
  51. 後藤寬貴・新美輝幸(2015) クワガタムシの大顎・カブトムシの角の発生メカニズム.「遺伝子から解き明かす昆虫の不思議な世界」(大場裕一・大澤省三・昆虫DNA研究会編), 悠書館, 東京. P411-447.
  52. 間瀬睦月・大出高弘・新美輝幸 (2015) カブトムシの性差決定遺伝子に関する研究―なぜオスだけに角ができるのか?. 昆虫と自然, 50, 27-29.
  53. Gotoh, H., Hust, J. A., Miura, T., Niimi, T., Emlen, D. J., Lavine, L. C.(2015) The Fat/Hippo signaling pathway links within-disc morphogen patterning to whole-animal signals during phenotypically plastic growth in insects. Dev. Dyn., 244, 1039–1045.
  54. 後藤寬貴・柳沼利信・新美輝幸(2015) カブトムシの角はどのように進化したのか?「進化の謎をゲノムで解く」(長谷部光泰監修), 秀潤社, 東京. P88-95.
  55. 新美輝幸(2015) カブトムシの雄特異的な角形成.生体の科学, 66, 228-233.