Chikami, Y., Okuno, M., Toyoda, A., Itoh, T. and Niimi, T. (2022) Evolutionary history of sexual differentiation mechanism in insects. Mol. Biol. Evol., 39, in press. DOI: 10.1093/molbev/msac145
Sakura, K., Morita, S. and Niimi, T. (2022) RNA interference method for gene function analysis in the Japanese rhinoceros beetle Trypoxylus dichotomus. Bio-protoc., 12, e4396. DOI: 10.21769/BioProtoc.4396
Ohde, T., Mito, T. and Niimi, T. (2022) A hemimetabolous wing development suggests the wing origin from lateral tergum of a wingless ancestor. Nat. Commun., 13, 979. DOI: 10.1038/s41467-022-28624-x.
Morita, S., Sakura, K., Gotoh, H., Emlen, D. J., Niimi, T. (2022) Recent advances in understanding horn formation in the Japanese rhinoceros beetle Trypoxylus dichotomus using next generation sequencing technology. Curr. Opin. Insect Sci., in press. DOI: 10.1016/j.cois.2022.100901
Shigenobu, S., Hayashi, Y., Watanabe, D. Tokuda, G., Hojo, M. Y., Toga, K., Saiki, R., Yaguchi, H., Masuoka, Y., Suzuki, R., Suzuki, S. Kimura, M. Matsunami, M., Sugime, Y., Oguchi, K., Niimi, T., Gotoh, H., Hojo, M. K., Miyazaki, S., Toyoda, A., Miura, T. and Maekawa, K. (2022) Genomic and transcriptomic analyses of the subterranean termite Reticulitermes speratus: Gene duplication facilitates social evolution. Proc. Natl. Acad. Sci. USA., 119, e2110361119. DOI: 10.1073/pnas.2110361119
Miyazaki, S., Fujiwara, K., Kai, K., Masuoka, Y., Gotoh, H., Niimi, T., Hayashi, Y., Shigenobu, S. and Maekawa, K. (2021) Evolutionary transition of doublesex regulation from sex-specific splicing to male-specific transcription in termites. Sci. Rep.,11, 15992. DOI: 10.1038/s41598-021-95423-7.
Hashiro, S., Chikami, Y., Kawaguchi, H., Krylov, A. A., Niimi, T. and Yasueda, H. (2021) Efficient production of long double-stranded RNAs applicable to agricultural pest control by Corynebacterium glutamicum equipped with coliphage T7-expression system. Appl. Microbiol. Biotechnol., 105, 4987-5000. DOI: 10.1007/s00253-021-11324-9
Chikami, Y., Kawaguchi, H., Suzuki, T., Yoshioka, H., Sato, Y., Yaginuma, T. and Niimi, T. (2021) Oral RNAi of diap1 results in rapid reduction of damage to potatoes in Henosepilachna vigintioctopunctata. J. Pest Sci., 94, 505–515. DOI 10.1007/s10340-020-01276-w
Niimi, T. and Ando, T. (2021) Evo-devo of wing colour patterns in beetles. Curr. Opn. Genet. Dev., 69, 97-102. DOI: doi.org/10.1016/j.gde.2021.02.007
Adachi, H., Matsuda, K., Niimi, T., Kondo, S., and Gotoh, H. (2020). Genetical control of 2D pattern and depth of the primordial furrow that prefigures 3D shape of the rhinoceros beetle horn. Sci. Rep. 10. DOI: 10.1038/s41598-020-75709-y
Sakai, H., Konagaya, T.,Takemura, Y.,Sahara, K. and Niimi, T. (2020) Double-copulated introduction of ejaculate with dominant larval phenotype to maintain Bombyx mori mutant with dysfunctional apyrene sperm. J. Insect Biotechnol. Sericol., 89, 39-43.DOI: doi.org/10.11416/jibs.89.2_039
(2020年度 日本蚕糸学会進歩賞 奨励賞)
Hashiro, S., Mitsuhashi, M., Chikami, Y., Kawaguchi, H., Niimi, T. and Yasueda, H. (2019) Construction of Corynebacterium glutamicum cells as containers encapsulating dsRNA overexpressed for agricultural pest control. Appl. Microbiol. Biotechnol., 103, 8485-8496. DOI: 10.1007/s00253-019-10113-9
Sakai, H., Oshima, H., Yuri, K., Gotoh, H., Daimon, T., Yaginuma, T., Sahara, K. and Niimi, T. (2019) Dimorphic sperm formation by Sex-lethal. Proc. Natl. Acad. Sci. USA., 116, 10412-10417.DOI: 10.1073/pnas.1820101116
Morita, S., Ando, T., Maeno, A., Mizutani, T., Mase, M., Shigenobu, S. and Niimi, T. (2019) Precise staging of beetle horn formation in Trypoxylus dichotomus reveals the pleiotropic roles of doublesex depending on the spatiotemporal developmental contexts. PLOS Genet., 15, e1008063. DOI: 10.1371/journal.pgen.1008063
Ando, T. and Niimi, T. (2019) Development and evolution of color patterns in ladybird beetles: A case study in Harmonia axyridis. Dev. Growth Differ., 61, 73-84. DOI: 10.1111/dgd.12592
Zinna, R., Emlen, D., Lavine, L. C., Johns, A., Gotoh, H., Niimi, T. and Dworkin, I. (2018) Sexual dimorphism and heightened conditional expression in a sexually selected weapon in the Asian rhinoceros beetle. Mol. Ecol., 27, 5049-5072. DOI: 10.1111/mec.14907
Sota, T., Sugawara, H., Fujisawa, T., Fujimaki, K. and Niimi, T. (2018) Knockdown of rotund gene through larval RNA interference affects genital and elytral morphology in the ground beetle Carabus maiyasanus (Coleoptera: Carabidae). Entomol. Sci., 21, 469-474. DOI: 10.1111/ens.12330
Ohde, T., Morita, S., Shigenobu, S., Morita, J., Mizutani, T., Gotoh, H., Zinna, R. A., Nakata, M., Ito, Y., Wada, K., Kitano, Y., Yuzaki, K., Toga, K., Mase, M., Kadota, K., Rushe, J., Lavine, L. C., Emlen, D. J. and Niimi, T. (2018) Rhinoceros beetle horn development reveals deep parallels with dung beetles. PLOS Genet., 14, e1007651. DOI: 10.1371/journal.pgen.1007651
Ando, T., Matsuda, T., Goto, K., Hara, K., Ito, A., Hirata, J., Yatomi, J., Kajitani, R., Okuno, M., Yamaguchi, K., Kobayashi, M., Takano, T., Minakuchi, Y., Seki, M., Suzuki, Y., Yano, K., Itoh, T., Shigenobu, S., Toyoda, A. and Niimi, T. (2018) Repeated inversions within a pannier intron drive diversification of intraspecific colour patterns of ladybird beetles. Nat. Commun., 9, 3843.DOI: 10.1038/s41467-018-06116-1
(Recommended by F1000)
Ohde, T., Takehana, Y., Shiotsuki, T. and Niimi, T. (2018) CRISPR/Cas9-based heritable targeted mutagenesis in Thermobia domestica: A genetic tool in an apterygote development model of wing evolution. Arthropod Struct. Dev., 47, 362-369. DOI: 10.1016/j.asd.2018.06.003
Kawaguchi, H. and Niimi, T. (2018) A method for cryopreservation of ovaries of the ladybird beetle, Harmonia axyridis. J. Insect Biotechnol. Sericol., 87, 35-44. DOI: https://doi.org/10.11416/jibs.87.2_035
Adachi, H., Matsuda, K., Niimi, T., Inoue, Y., Kondo, S. and Gotoh, H. (2018) Anisotropy of cell division and epithelial sheet bending via apical constriction shape the complex folding pattern of beetle horn primordia. Mech. Dev., 152, 32-37. DOI: 10.1016/j.mod.2018.06.003
Tsuji, T., Gotoh, H., Morita, S. Hirata, J., Minakuchi, Y., Yaginuma, T., Toyoda, A. and Niimi, T. (2018) Molecular characterization of eye pigmentation-related ABC transporter genes in the ladybird beetle Harmonia axyridis reveals striking gene duplication of the white gene. Zool. Sci.,35, 260-267. DOI: 10.2108/zs170166
Hust, J., Lavine, M. D., Worthington, A. M., Zinna, R., Gotoh, H., Niimi, T. and Lavine, L. (2018) The Fat-Dachsous signaling pathway regulates growth of horns in Trypoxylus dichotomus, but does not affect horn allometry. J. Insect Physiol., 105, 85-94. DOI: 10.1016/j.jinsphys.2018.01.006
Zinna, R., Gotoh, H., Kojima, T. and Niimi, T. (2018) Recent advances in understanding the mechanisms of sexually dimorphic plasticity: Insights from beetle weapons and future directions. Curr. Opi. Insect Sci., 25, 35–41. DOI: 10.1016/j.cois.2017.11.009
Matsuda, K., Gotoh, H., Tajika, Y., Sushida, T., Aonuma, H., Niimi, T., Akiyama, M., Inoue, Y. and Kondo, S. (2017) Complex furrows in a 2D epithelial sheet code the 3D structure of a beetle horn. Sci. Rep., 7, 13939. DOI: 10.1038/s41598-017-14170-w
Ozawa, T., Mizuhara, T., Arata, M., Shimada, M., Niimi, T., Okada, K., Okada, Y. and Ohta, K. (2016) Histone deacetylases control module-specific phenotypic plasticity in beetle weapons. Proc. Natl. Acad. Sci. USA., 113, 15042-15047.DOI: 10.1073/pnas.1615688114
Zinna, R., Gotoh, H., Brent, C. S., Dolezal, A., Kraus, A., Niimi, T., Emlen, D. and Lavine, L. C. (2016) Endocrine control of exaggerated trait growth in rhinoceros beetles. Integr. Comp. Biol., 56, 247-259.doi: DOI: 10.1093/icb/icw042
Gotoh, H., Ishiguro, M., Nishikawa, H., Morita, S., Okada, K., Miyatake, T., Yaginuma T. and Niimi, T. (2016) Molecular cloning and functional characterization of the sex-determination gene doublesex in the sexually dimorphic broad-horned beetle Gnatocerus cornutus (Coleoptera, Tenebrionidae). Sci. Rep., 6, 29337.DOI: 10.1038/srep29337
Gotoh, H., Zinna, R. A., Warren, I., DeNieu, M., Niimi, T., Dworkin, I., Emlen, D. J., Miura, T. and Lavine, L. C. (2016) Identification and functional analyses of sex determination genes in the sexually dimorphic stag beetle Cyclommatus metallifer. BMC Genomics, 17, 250. DOI: 10.1186/s12864-016-2522-8
Hatakeyama, M., Yatomi, J., Sumitani, M., Takasu, Y., Sekiné, K., Niimi, T. and Sezutsu, H. (2015) Knockout of a transgene by transcription activator-like effector nucleases (TALENs) in the sawfly, Athalia rosae (Hymenoptera) and the ladybird beetle, Harmonia axyridis (Coleoptera). Insect Mol. Biol.,25,24-31.
Gotoh, H., Hust, J. A., Miura, T., Niimi, T., Emlen, D. J., Lavine, L. C.(2015) The Fat/Hippo signaling pathway links within-disc morphogen patterning to whole-animal signals during phenotypically plastic growth in insects. Dev. Dyn.,244, 1039–1045.