The 8th NIBB International Practical Course
The 3rd NIBB-TLL-DBS/NUS Joint International Practical Course

Experimental Techniques using
Medaka and Xenopus
– The Merits of using both –

Top
Message
Program
Application
Teachers
Access
Accommodation
Organizers

Program

 

Program (Tentative)

 

Mon, September 22nd, 2014

14:00 - 16:00 Arrival and Registration
16:00 - 16:30 Opening Remarks & Orientation
16:30 - 17:30 Seminar by Dr. Yoshimura, Takashi (Nagoya Univ./NIBB)
18:00 - 20:00 Welcome Party

 

Tue, September 23rd, 2014

09:00 - 10:00 Outline for procedure of TALEN by Dr. Sakuma
10:00 - 10:30 Outline for Xenopus manipulation by Drs. Suzuki, Miyamoto and Teperek
10:30 - 12:00 Manipulation of Xenopus embryo
13:00 - 16:30 Manipulation of Xenopus embryo
16:30 - 17:30 Seminar by Dr. Winkler, Christoph (Univ. of Singapore, Singapore)
18:00 - 20:00 Social Hour

   

Wed, September 24th, 2014

09:00 - 09:30 Outline for microinjection in Xenopus by Drs. Suzuki, Miyamoto and Teperek
09:30 - 12:00 Microinjection of TALEN mRNA in Xenopus egg
13:00 - 16:30 in vitro fertilization in Xenopus tropicalis
16:30 - 17:30 Seminar by Dr. Miyamoto, Kei (Wellcome Trust/Cancer Research UK Gurdon Institute)
18:00 - 20:00 Social Hour

　 　

Thu, September 25th, 2014

09:00 - 09:30 Outline for procedure of CRISPR/Cas9 by Dr. Kinoshita
09:30 - 12:00 in vitro transcription of sgRNA
13:00 - 17:00 sgRNA purification
17:00 - 18:00 Seminar by Dr. Thumberger, Thomas (Heidelberg Univ., Germany)
 

Fri, September 26th, 2014

09:00 - 09:30 Outline for microinjection in medaka by Dr. Kinoshita
09:30 - 12:00 Microinjection of sgRNA and Cas9 mRNA into medaka egg
13:00 - 17:00 Observation of artificially inseminated Xenopus embryos
17:00 - 18:00 Seminar by Dr. Kamei, Yasuhiro (NIBB)
 

Sat, September 27th, 2014

09:00 - 09:30 Outline for procedure of Cryopreservation by Dr. Sasado
09:30 - 12:00 Dissection of Testis/Sperm Freezing
13:00 - 13:30 Outline for procedure of Insemination by Dr. Sasado
13:00 - 17:00 Insemination/Observation
17:00 - 18:00 Seminar by Dr. Takeuchi, Hideaki (Univ. of Tokyo)

　 　

Sun, September 28th, 2014

 

Mon, September 29th, 2014

09:00 - 10:30 Observation of TALEN or CRISPR/Cas9 induced mutants
10:30 - 11:00 Outline for procedure of IR-LEGO by Drs. Yokoyama and Kamei
11:00 - 12:00 in vivo Cell Manipulation using IR-LEGO
13:00 - 17:00 in vivo Cell Manipulation using IR-LEGO
17:00 - 18:00 Seminar by Dr. Yokoyama, Hitoshi (Tohoku Univ.)

 

Tue, September 30th, 2014

09:00 - 09:30 Outline for procedure of Live Imaging by Drs. Nonaka and Kamei
09:30 - 12:00 Live Imaging
13:00 - 14:00 Seminar by Dr. Ogino, Hajime (Nagahama Institute of Bio-Science and Technology)
14:00 - 17:00 Live Imaging
17:00 - 18:00 Seminar by Dr. Hayashi, Toshinori (Tottori Univ.)
 

Wed, October 1st, 2014

09:00 - 10:00 Outline for PCR genotyping of CRISPR/Cas9 induced mutants by Dr. Kinoshita
10:00 - 12:00 DNA extraction and PCR
13:00 - 17:30 HRM/Electrophoresis
18:00 - Farewell Party
 

Thu, October 2nd, 2014

Departure