The 8th NIBB International Practical Course
The 3rd NIBB-TLL-DBS/NUS Joint International Practical Course

Experimental Techniques using
Medaka and Xenopus
– The Merits of using both –

The 8th NIBB International Practical Course<br>The 3rd NIBB-TLL-DBS/NUS Joint International Practical Course
Contents:
“Genome Editing”
Gene knock-out using TALEN in Xenopus
by Tetsushi SAKUMA and Ken-ichi T Suzuki (Hiroshima Univ.), Kei MIYAMOTO and Marta TEPEREK (Wellcome Trust/Cancer Research UK Gurdon Institute)
Gene knock-out using CRISPR/CAS9 system in medaka
by Masato KINOSHITA (Kyoto Univ.)
Gene knock-in using CRISPR/CAS9 system in medaka
by Thomas THUMBERGER (Heidelberg Univ.)

“Manipulation of Xenopus tropicalis Eggs and Embryos”
in vitro fertilization of eggs and manipulation of embryos
by Akihiko KASHIWAGI and Keiko KASHIWAGI (Hiroshima Univ.)

“In Vivo Cell Manipulation”
Local gene induction with the infrared laser-evoked gene operator (IR-LEGO) method by Yasuhiro KAMEI (NIBB) and Hitoshi YOKOYAMA (Tohoku Univ.)

“Live Imaging”
2-photon microscopy and Digital Scaning Light-sheet Microscopy (DSLM)
by Shigenori NONAKA and Yasuhiro KAMEI (NIBB)

“Strain Preservation”
Cryopreservation of sperm and artificial insemination for Medaka
by Takao SASADO (NIBB)

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