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文部科学省科学研究費補助金「特定領域研究」細胞核ダイナミクス
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時計タンパク質の核移行と核におけるリズム発振機能
しきり線
研究分担者
   
小内 清(研究分担者)
<国立大学法人名古屋大学・遺伝子実験施設・研究員>
〒464-8602 愛知県名古屋市千種区不老町
TEL: 052-789-4527; FAX: 052-789-4526
E-mail: onai@gene.nagoya-u.ac.jp
URL: http://www.gene.nagoya-u.ac.jp/~ishiura-g/men-onai.html

・主な研究内容
・研究室メンバー
・最近の研究成果
・総説・解説等
 
主な研究内容
(1)生物発光リアルタイム測定・スクリーニングシステムの開発
(2)植物時計の解明
 
研究室メンバー
 
 
最近の研究成果

Matsuo T, Onai K, Okamoto K, Minagawa J, Ishiura M (2006): Real-time monitoring of the circadian gene expression in the chloroplast. Mol. Cell. Biol. 26:863-870.

Yoine M, Ohto M, Onai K, Mita S, Nakamura K. (2006): The lba1 mutation of UPF1 RNA helicase involved in nonsense-mediated mRNA decay causes pleiotropic phenotypic changes and altered sugar signaling in Arabidopsis. Plant J. 47:49-62.

Onai K., Ishiura M. (2005): PHYTOCLOCK 1 encoding a novel GARP protein essential for the Arabidopsis circadian clock. Genes Cells 10:963-972.

Okamoto K, Onai K (共著第一著者), Furusawa T, Ishiura M (2005): A portable integrated automatic apparatus for the real-time monitoring of bioluminescence in plants. Plant Cell Environ. 28:1305-1315.

Okamoto K, Onai K, Ezaki N, Ofuchi T, Ishiura M (2005): An automated apparatus for the real-time monitoring of bioluminescence in plants. Anal. Biochem. 340:187-192.

Okamoto K, Onai K, Ishiura M (2005): RAP, an integrated program for monitoring bioluminescence and analyzing circadian rhythms in real time. Anal. Biochem. 340:193-200.

Iwase R, Imada K, Hayashi F, Uzumaki T, Morishita M, Onai K, Furukawa Y, Namba K, Ishiura M (2005): Unique homo-tetrameric structure and functionally important sub-structures of the circadian clock protein KaiB. J. Biol. Chem. 280:43141-43149.

Onai K, Okamoto K, Nishimoto H, Morioka C, Hirano M, Kami-ike N, Ishiura M (2004): Large-scale screening of Arabidopsis circadian clock mutants by a high-throughput real-time bioluminescence monitoring system. Plant J. 40:1-11.

Onai K, Morishita M, Itoh S, Okamoto K, Ishiura M (2004): Circadian rhythms in the thermophilic cyanobacterium Thermosynechococcus elongatus: compensation of period length over a wide temperature range. J. Bacteriol. 186:4972-4977.

Onai K, Morishita M, Kaneko T, Tabata S, Ishiura M (2004): Natural transformation of the thermophilic cyanobacterium Thermosynechococcus elongatus BP-1: a simple and efficient method for gene transfer. Mol. Genet. Genomics 271:50-59.

Shimada H, Ohno R, Shibata M, Ikegami I, Onai K, Ohto M, Takamiya K (2004): Inactivation and deficiency of core proteins of photosystems I and II caused by genetical phylloquinone and plastoquinone deficiency but retained lamellar structure in a T-DNA mutant of Arabidopsis. Plant J. 41:627-637.

Hayashi F, Ito N, Uzumaki T, Iwase R, Tsuchiya Y, Yamakawa H, Morishita M, Onai K, Itoh S, Ishiura M (2004): Roles of two ATPase-motif-containing domains in cyanobacterial circadian clock protein KaiC. J. Biol. Chem. 279:52331-52337

総説・解説等

 

         
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