ホーム
  領域代表者挨拶
  研究概要
  班員紹介
  シンポジウム
  技術紹介
  海外の動き
  国内の動き
  関連書籍
  サーキュラー
  画像集
  リンク
文部科学省科学研究費補助金「特定領域研究」細胞核ダイナミクス
    戻る    
時計タンパク質の核移行と核におけるリズム発振機能
しきり線
研究代表者
   
石浦 正寛(研究代表者)
<国立大学法人名古屋大学・遺伝子実験施設・教授>
〒464-8602 愛知県名古屋市千種区不老町
TEL: 052-789-4527; FAX: 052-789-4526
E-mail: ishiura@gene.nagoya-u.ac.jp
URL: http://www.gene.nagoya-u.ac.jp/~ishiura-g/

・主な研究内容
・研究室メンバー
・最近の研究成果
・総説・解説等
 
主な研究内容
(1)藍色細菌の時計機構の原子レベルでの解明
(2)高等植物の時計機構の解明
(3)植物時計の進化様式の解明
(4)生物発光を利用したリアルタイム測定法の開発
 
研究室メンバー
 
 
最近の研究成果

Hayashi F, Iwase R, Uzumaki T, Ishiura M (2006): Hexamerization by the N-terminal domain and intersubunit phosphorylation by the C-terminal domain of cyanobacterial circadian clock protein KaiC. Biochem Biophys Res Commun 348:864-872.

Matsuo T, Onai K, Okamoto K, Minagawa J, Ishiura M (2006): Real-time monitoring of the circadian gene expression in the chloroplast. Mol Cell Biol 26:863-870.

Iwase R, Imada K, Hayashi F, Uzumaki T, Morishita M., Onai K, Furukawa Y., Namba K, Ishiura M (2005): Functionally important substructures of circadian clock protein KaiB in unique tetramer complex. J Biol Chem 280:43141-43149.

Onai K, Ishiura M (2005): PHYTOCLOCK 1 encoding a novel GARP protein esstial for the Arabidopsis circadain clock. Genes Cells 10:963-972.

Okamoto K, Onai K, Furusawa T, Ishiura M (2005): A portable integrated automatic apparatus for the real-time monitoring of bioluminescence in plants. Plant Cell Environ 28:1305-1315.

Kutsuna S, nakahira Y, Katayama M, Ishiura M, Kondo T (2005): Transcriptional regulation of the circadian clock operon kaiBC by upstream regions in cyanobacteria. Mol Microbiol 57:1474-1484.

Okamoto K, Onai K, Ishiura M (2005): RAP, an integrated program for monitoring bioluminescence and analyzing circadian rhythms in real time. Anal Biochem 340:193-200.

Okamoto K, Onai K, Ezaki N, Ofuchi T, Ishiura M (2005): An automated apparatus for the real-time monitoring of bioluminescence in plants. Anal Biochem 340:187-192.

Kucho K, Okamoto K, Tabata S, Fukuzawa H, Ishiura M (2005): Identification of novel clock-regulated genes by cDNA macroarray analysis in Chlamydomonas reinhardtii. Plant Mol. Biol 57:889-906.

Kucho K, Okamoto K, Tsuchiya Y, Nomura S, Nango M, Kanehisa M, Ishiura M (2005): Global analysis of circadian expression in the cyanobacterium Synechocystis sp. strain PCC 6803. J Bacteriol 187:2190-2199.

Kucho K, Aoki K, Itoh S, Ishiura M (2005): Improvement of the bioluminescence reporter system for real-time monitoring of circadian rhythms in the cyanobacterium Synechocystis sp. strain PCC 6803. Genes Genet Sys 80:19-23.

Hayashi F, Ito N, Uzumaki T, Iwase R, Tsuchiya Y, Yamakawa H, Morishita M, Onai K, Itoh S, Ishiura M (2004): Roles of two ATPase-motif-containing domains in cyanobacterial circadian clock protein KaiC. J Biol Chem 279:52331-52337.

Kucho K, Tsuchiya Y, Okumoto K, Harada M, Yamada M, Ishiura M (2004): Construction of unmodified oligonucleotide-based microarray in the thermophilic cyanobacterium Thermosynechococcus elongatus BP-1. Genes Genet Sys 79:319-329.

Onai K, Okamoto K, Nishimoto H, Morioka C, Hirano M, Kami-ike N, Ishiura M (2004): Large-scale screening of Arabidopsis circadian clock mutants by a high-throughput real-time bioluminescence monitoring system. Plant J 40:1-11.

Kucho K, Yoneda H, Harada M, Ishiura M (2004): Determinants of sensitivity and specificity in spotted DNA microarrays with unmodified oligonucleotides. Genes Genet Sys 79:189-197.

Onai K, Morishita M, Itoh S, Okamoto K, Ishiura M (2004): Circadian rhythms in the thermophilic cyanobacterium Thermosynechococcus elongatus: compensation of period length over a wide temperature range. J Bacteriol 186:4972-4977.

Uzumaki T, Fujita M, Nakatsu T, Hayashi F, Shibata H, Itoh N, Kato H, Ishiura M (2004): Crystal structure of the C-terminal clock-oscillator domain of the cyanobacterial KaiA protein. Nature Struct Mol Biol. 11:623-631.

Iwase R, Imada K, Hayashi F, Uzumaki T, Namba K, Ishiura M (2004): Crystallization and preliminary crystallographic analysis of the circadian clock protein KaiB from the thermophilic cyanobacterium Thermosynechococcus elongatus BP-1. Acta Crystal D 60:727-729.

Hayashi F, Ito H, Fujita M, Iwase R, Uzumaki T, Ishiura M (2004): Stoichiometric interactions between cyanobacterial clock proteins KaiA and KaiC. Biochem Biophys Res Commun 316:195-202.

Onai K, Morishita M, Kaneko T, Tabata S, Ishiura M (2004): Natural transformation of the thermophilic cyanobacterium Thermosynechococcus elongatus BP-1: a simple and efficient method for gene transfer. Mol Gen Genomics 271:50-59.

総説・解説等

 

         
    Copyright (c) 2004-2009, nuclear-dynamics. All Rights Reserved.