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文部科学省科学研究費補助金「特定領域研究」細胞核ダイナミクス
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核内脂質シグナル及び核移行蛋白質の可視化システムを用いた細胞周期制御機構の解明
しきり線
研究代表者
   
白井 康仁(研究代表者)
<神戸大学・バイオシグナル研究センター・助教授>
TEL: 078-802-5962 FAX: 078-802-5971

・主な研究内容
・研究室メンバー
・最近の研究成果
白井 康仁
主な研究内容
・可視化システムを用いたPKCとDGKの機能解析及び機能協関
・核内脂質動態と脂質関連酵素の核移行
・PKC及びDGKに認められる脂質シグナル結合領域の高次構造解析
 
研究室メンバー
・斎藤尚亮(教授)
・白井康仁(助教授)
・上山健彦(助手)
・大森志保(ポスドク)
・柏木香保里(ポスドク)
・高橋英之 (ポスドク)
・川ア拓実(D2)
・足立直子(D1)
・松原岳大(D1)
・辰の敏彦(M2)
・筧朋子(M2)
・三畝宏一(M2)
・倉増真帆(M1)
・濱崎健太郎(M1)
・鵜尾道秀(4年)
・盛岡祥子(4年)
・辻部智(4年)
・山口泰人(研究生)
・高岡幸二(研究生)
 
最近の研究成果

Shirai Y, Balsinde J. and Dennis E. (2005) Localization and Functional Interrelationships Among Cytosolic Group IV, Secreted Group V, and Ca2+-Independent Group VI Phospholipase A2s in P388D1 Macrophages Using GFP/RFP Constructs. Biochemica Biocphyca Acta, lipid 1735, 119-129.

Fukunaga-Takenaka, R., Shirai, Y., Yagi, K., Adachi, N., Sakai, N., Merino, E., Merida, I., and Saito, N. (2005) Importance of chroman ring and tyrosine phosphorylation in the subtype-specific translocation and activation of diacylglycerol kinase alpha by d-α-tocophenol. Genes to Cells. 10, 311-319.

Kuriyama M, Taniguchi T, Shirai Y, Sasaki A, Yoshimura A and Saito N (2004) Activation and translocation of PKC is necessary for VEGF-induced ERK activation through KDR in HEK293T cells. Biochem. Biophys. Res. Comm. 325, 843-851.

Seki T, Matsubayashi H, Amano T, Shirai Y, Saito N and Sakai N (2004) Phosphorylation of PKC activation loop plays an important role in receptor-mediated translocation of PKC Genes to cells 10, 945-957.

Sakai N, Tsubokawa H, Matsuzaki M, Kajimoto T, Takahashi E, Ren Y, Ohmori S, Shirai Y, Matsubayashi H, Chen J, Duman RS, Kasai H, Saito N. (2004) Propagation of gammaPKC translocation along the dendrites of Purkinje cell in gammaPKC-GFP transgenic mice. Genes to cells 9, 945-957.

Ueyama T, Lennartz MR, Noda Y, Kobayashi T, Shirai Y, Rikitake K, Yamasaki T, Hayashi S, Sakai N, Seguchi H, Sawada M, Sumimoto H, Saito N. (2004) Superoxide production at phagosomal cup/phagosome through betaI protein kinase C during FcgammaR-mediated phagocytosis in microglia. J Immunol. 173, :4582-4589.

Kajimoto T, Shirai Y, Sakai N, Yamamoto T, Matsuzaki H, Kikkawa U, and Saito N (2004) Ceramide-induced apoptosis by translocation, phosphorylation and activation of protein kinase Cδ in the Golgi complex. J. Biol. Chem. 279, 12668-12676.

Yagi K, Shirai Y, Hirai M, Sakai N and Saito N. (2004) Phospholipase A2 products retain a neuron specificγ isoform of PKC on the plasma membrane through the C1 domain- a molecular mechanism for sustained enzyme activity. Neurochem Inter. 45, 39-47

Uchino M, Saka N, Kashiwagi K, Shirai Y, Shinohara Y, Hirose K, Iino M, Yamamura T, and Saito N (2003) Isoform-specific phosphorylation of mGluR5 by PKC blocks Ca2+ oscillation and oscillatory translocation of Ca2+-dependent PKC. J. Biol. Chem., 279: 2254 - 2261.

Balboa M, Shirai Y, Gaietta G, Ellisman M, Balsinde J and Dennis E. (2003) Localization of Group V Phospholipase A2 in Caveolin-enriched Granules in Activated P388D1 Macrophage-like Cells. J. Biol. Chem., 278: 48059 - 48065.

Shindo M., Irie K., Masuda A., Ohigashi H., Shirai Y., Myasaka K., and Naoaki Saito (2003) Synthesis and Phrobol ester biniding of the cysteine-rich domains of diacylglycerol kinase (DGK) isozymes. J. Biol. Chem. 278, 18448-18454.

Sakai K., Hasegawa C., Okura M., Morikawa O., Ueyama T., Shirai Y., Sakai N. and Saito N. (2003) Novel variants of murine serotonin transporter mRNA and the promoter activity of its upstream site. Neurosci. Lett. 342, 175-178.

Irie N., Sakai N., Ueyama T., Kajimoto T., Shirai Y. and Saito N (2002) Subtype- and species-specific knocckdown of PKC using short interfering RNA (siRNA) Biochem. Biophys. Res. Comm. 298, 738-743.

Larsen C.E, Ueyama T, Brannock M.P., Shirai Y., Saito N, Larsson C., Daniel Loegering D., Weber B.P. and Lennartz R.M. (2002) A Role for PKC- in Fcγ R-mediated phagocytosis by RAW 264.7 cells. J. Cell Biol. 159, 939-944.

         
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