-文部科学省科学研究費補助金「特定領域研究」細胞核ダイナミクス　大山　隆-

細胞核ダイナミクスにおける遺伝子の機能的収納機構の解明

研究代表者

大山　隆（研究代表者）＜早稲田大学教育・総合科学学術院・理学科生物学専修・教授＞ TEL: 03-5286-1520 E-mail: ohyama@waseda.jp ・主な研究内容・研究室メンバー・最近の研究成果・最近の主な総説・最近の著書

主な研究内容

・機能的クロマチンの構築機構
・ゲノムDNAの高次構造に印された遺伝情報の解読
・ゲノムDNAの機械的特性が担う遺伝情報の解読
・クロマチン工学の開拓
・食虫植物の腺細胞の個性を決めるエピジェネティクな制御機構

研究室メンバー

・岡部隆宏（Ｄ３）
・福江善朗（Ｄ３）
・井上正太郎（Ｄ３）
・隅田周志（Ｄ３）
・棚瀬潤一（M２）
・二谷知佳（Ｍ２）
・古屋貴代（Ｍ２）
・井上慎也（Ｍ１）
・元山照章（Ｍ１）

最近の研究成果

Fukue, Y., Sumida, N., Tanase, J. and Ohyama, T.: A highly distinctive mechanical property found in the majority of human promoters and its transcriptional relevance. Nucl. Acids Res. 33, 3821-3827 (2005).

Fukue, Y., Sumida, N., Nishikawa, J. and Ohyama, T.: Core promoter elements of eukaryotic genes have a highly distinctive mechanical property. Nucl. Acids Res. 32, 5834-5840 (2004).

Nishikawa, J., Amano, M., Fukue, Y., Tanaka, S., Kishi, H., Hirota, Y., Yoda, K. and Ohyama, T.: Left-handedly curved DNA regulates accessibility to cis-DNA elements in chromatin. Nucl. Acids Res. 31, 6651-6662 (2003).

Asayama, M., Kato, H., Shibata, J.,Shirai, M. and Ohyama, T.: The curved DNA structure in the 5'-upstream region of the light-responsive genes: its universality, binding factors and function for cyanobacterial psbA transcription. Nucl. Acids Res. 30, 4658-4666 (2002).

Tagashira, H., Morita, M. and Ohyama, T.: Electrophoretic mobility shift of restriction fragments caused by base pairing between overhangs. Biochemistry 41, 12217-12223 (2002).

Kusakabe, T., Sugimoto, Y., Maeda, T., Miyano, M., Nishikawa, J., Tone, S., Kawaguchi, Y., Koga, K. and Ohyama, T.: Linearization and integration of DNA into cells preferentially occurs at intrinsically curved regions from human LINE-1 repetitive element. Gene 274, 271-281 (2001).

最近の主な総説

Asayama, M. and Ohyama, T.: Curved DNA and prokaryotic promoters: a mechanism for activation of transcription. In: Ohyama, T. (ed.), DNA Conformation and Transcription. Springer (New York) and Landes Bioscience (Texas), 37-51 (2005).

Ohyama, T.: Curved DNA and Transcription in eukaryotes. In: Ohyama, T. (ed.), DNA Conformation and Transcription. Springer (New York) and Landes Bioscience (Texas), 66-74 (2005).

Ohyama, T.: The role of unusual DNA structures in chromatin organization for transcription. In: Ohyama, T.(ed.), DNA Conformation and Transcription. Springer (New York) and Landes Bioscience (Texas), 177-188 (2005).

Ohyama, T.: Intrinsic DNA bends: an organizer of local chromatin structure for transcription. BioEssays 23, 708-715 (2001).

Ohyama, T.: Regulation of chromatin structure by curved DNA: how activator binding sites become accessible. In: Nagata, K. and Takeyasu, K. (eds.), Nuclear Dynamics: Approaches from Molecular, Biochemical and Visual Biology. Springer-Verlag (Tokyo), in press.

最近の著書

Ohyama, T. (ed.)「DNA Conformation and Transcription」(分担執筆) Springer (New York) and Landes Bioscience (Texas), (2005).

Nagata, K. and Takeyasu, K. (eds.)「Nuclear Dynamics: Approaches from Molecular, Biochemical and Visual Biology」（分担執筆）Springer-Verlag (Tokyo), in press.

＊執筆部分の内容は総説欄に記載。