Plasmids are available upon request to Mitsuyasu Hasebe (mhasebe@nibb.ac.jp). Most plasmids are distributed before publication as a volunteer for convenience of Physco friends without any funds.
We found that 7133 promoter we has been used has a sequence of 7113 promoter. The original plasmid we obtained from Drs. Mitsuhara and Ohashi has the 7113 plasmid with a label of 7133 on the tube. Therefore all plasmids we used in my laboratory as 7133 should be 7113.
No |
Name |
marker |
comment |
1 |
nptII |
G418 resistant
cassette |
|
2 |
nptII |
Modified nptII
cassette |
|
3 |
nptII |
Modified nptII
cassette |
|
4 |
nptII |
Floxed modified nptII
cassette |
|
5 |
nptII |
Floxed modified nptII
cassette, MCS variant |
|
6 |
aph4 |
Floxed modified aph4
cassette |
|
7 |
aph4 |
Floxed modified aph4
cassette, MCS varinat |
|
8 |
ble |
Zeocin
resistant cassette |
|
9 |
zeocin |
Floxed
modified zeocin resistant cassette |
|
10 |
Blastcidin
S |
Floxed modified
blastcidin S resistant cassette |
No |
Name |
reporter |
marker |
comment |
1 |
uidA |
nptII |
Modified nptII
cassette: to fuse GUS to a C terminal |
|
2 |
uidA |
nptII |
Floxed modified nptII
cassette: to fuse GUS to a C terminal |
|
3 |
uidA |
nptII |
GUS (M1L), Floxed
modified nptII cassette: to fuse GUS to a C terminal |
|
4 |
gfp |
nptII |
GFP(M1L): to fuse GFP
to a C terminal |
|
5 |
gfp |
aph4 |
GFP(M1L): to fuse GFP
to a C terminal |
|
6 |
gfp |
aph4 |
GFP(M1L), linker
sequence just before GFP;@ to fuse GFP
to a C terminal |
|
7 |
gfp |
Ble |
GFP(M1L): to fuse GFP
to a C terminal |
|
8 |
gfp |
ble |
to
fuse GFP to a N terminal |
|
9 |
citrine |
nptII |
To
fuse citrine to either terminal |
3.
Plasmids for overexpression analysis
No |
Name |
Promoter |
marker |
Target
site |
Comment
& references |
1 |
E7113 |
nptII |
PpMAD2 |
@ |
|
2 |
E7113 |
nptII |
PpMAD2 |
|
|
3 |
E7113 |
nptII |
Pphb7 |
|
|
4 |
E7113 |
nptII |
Pphb7 |
|
|
5 |
Actin |
nptII |
PpMAD2 |
|
|
6 |
Actin |
nptII |
PpMAD2 |
|
|
7 |
Actin |
nptII |
Pphb7 |
|
|
8 |
Actin |
nptII |
Pphb7 |
|
|
9 |
Actin |
ble |
213 locus |
GFP-fusion |
|
10 |
7113 |
ble |
213 locus |
|
|
11 |
7113 |
ble |
213 locus |
|
|
12 |
CaMV35S |
ble |
213 locus |
mRFP-fusion |
|
13 |
CaMV35S |
ble |
213 locus |
mRFP-fusion |
|
14 |
Actin |
nptII |
|
GFP-fusion |
|
15 |
Actin |
nptII |
|
GFP-fusion |
|
16 |
Actin |
nptII GFP |
|
|
|
17 |
Actin |
None |
|
Transient expression |