pGFPmutNPT

Designed and constructed by Rumiko Kofuji

Primers

sGFP- (308): 5’- TCGCCGGACACGCTGAACTTGTG-3’

Sequence

>5’MCS:

TTG GGT ACC GGG CCC CCC CTC GAG GTC GAC GGT ATC GAT AAG CTT TTG GTG AG

>3’MCS:

AAAGATAAAAGATCTTTCAAGATCAAAACTAGTTCCCTCACACCGGTGACGGGGATCGCATGCGATATCTCGAGGGGGATCCACTAGTTCTAGAGCGGCCGCCACCGCGGTGGAGCTCCAGCTTTTGTTCCCTTTAG