pTN85

Designed and constructed by Tomoaki Nishiyama

Primers

GUS-R2 (308): 5’- ATTTCACGGGTTGGGGTTTCTACAGGACGT-3’

GUS-R3 (308): 5’- TCTTGTAACGCGCTTTCCCACCAACGCTGA-3’

M13-21: 5’-TGT AAA ACG ACG GCC AGT-3’

Reverse: 5’-AGC GGA TAA CAA TTT CAC ACA GG-3’

Sequence:

>5’ MCS

CGCGCAATTAACCCTCACTAAAGGGAACAAAAGCTGGGTACCGGGCCCCCCCTCGAGGTCGACGGTATCGATAAGCTTTTGTTACGTCCTGTAGAAACCCCAACCCGTGAAATCAAAAAA

>3’ MCS

CCGGTGACGGGGATCGGGGGGATCCACTAGTTCTAGAGCGGCCGCCACCGCGGTGGAGCTCCAAT