Program
| April 20 (Monday) |
| 09:00-09:20 |
Registration |
| 09:20-09:30 |
Opening Remarks Kiyotaka Okada (Director-General, NIBB) |
| Structural Biology and Functional Imaging
|
| 09:30-10:00 |
Stephen Cusack (EMBL Grenoble)
"Imaging Influenza Virus Polymerase in vitro and in vivo" |
| 10:00-10:30 |
Michael Sattler (Helmholtz Zentrum München & TU München)
"Molecular Recognition and Dynamics of Protein Complexes in Splicing Regulation" |
| 10:30-10:50 |
Coffee break |
| 10:50-11:20 |
Masahide Kikkawa (Kyoto Univ.)
"Structure and Mechanism of Microtubule-Based Motors" |
| 11:20-11:50 |
Kazuhiro Maeshima (NIG)
"How Is Genome DNA Compacted into a Mitotic Chromosome?" |
| 11:50-12:50 |
Lunch |
| Electron Microscopy and Functional Imaging
|
| 12:50-13:20 |
Kuniaki Nagayama (OIB)
"Nano-Bioimaging by Phase Contrast Electron Cro-tomography" |
| 13:20-13:50 |
Achilleas Frangakis (Univ. Frankfurt)
"The Native Organization of Desmosomes" |
| 13:50-14:20 |
Thomas Müller-Reichert (Max Planck Institute)
"Correlative Light Microscopy and Electron Tomography of the Early C. elegans Embryo" |
| 14:20-14:40 |
Coffee break |
| 14:40-15:10 |
Tokuko Haraguchi (KARC)
"Live CLEM: A New Approach for Observing Molecular Dynamics in High Resolution" |
| 15:10-15:25 |
Xavier Heiligenstein (EMBL Heidelberg) *Short talk from posters
“High Resolution Map of the Spindle Pole Body from S. Cerevisiae by Electron Tomography of Frozen Hydrated Sections” |
| 15:25-15:35 |
Short break |
| Functional Imaging in Live Cells (I)
|
| 15:35-16:05 |
Junichi Nabekura (NIPS)
"Microglia Surveillance of Synapses: in vivo Observation" |
| 16:05-16:35 |
Akihiko Nakano (Univ. Tokyo / RIKEN ASI)
"High-Resolution Confocal Imaging to Visualize Dynamic Membrane Trafficking in Living Cells" |
| 16:35-16:50 |
Amanda Cobos Correa (EMBL Heidelberg) *Short talk from posters
“New Fluorescent Tools to Image MMP-12 Activity in Lung Inflammation” |
| 16:50-17:05 |
Nobuyuki Shiina (OIB) *Short talk from posters
“Visualization of Na+ in Neurons Deficient in RNG105, a Factor Involved in Dendritic Local Translation” |
| 17:05-17:20 |
Naoshi Hattori (Univ. Tokyo) *Short talk from posters
“Functional Analysis of Arf GAP Family Proteins by Reconstitution of Split Fluorescent Proteins” |
| 17:30-18:30 |
Exhibition (Hamamatsu Photonics, Nikon, Olympus,Yokogawa Electric) |
| 18:30-20:00 |
Mixer |
| April 21 (Tuesday) |
| Functional Imaging in Live Cells (II)
|
| 09:00-09:30 |
Atsushi Miyawaki (RIKEN BSI / ERATO, JST)
"New Fluorescent Probes and New Perspectives in Bioscience" |
| 09:30-10:00 |
Carsten Schultz (EMBL Heidelberg)
"Novel Probes to Analyze Cellular Events with Spatial Resolution " |
| 10:00-10:20 |
Coffee break |
| Functional Imaging in Whole Animals
|
| 10:20-10:50 |
Ernst H. K. Stelzer (EMBL Heidelberg)
"Light Sheet Based Fluorescence Microscopy in Modern Biology" |
| 10:50-11:20 |
Norihiro Sadato (NIPS)
"The Neural Basis of Social Reward and Decision-Making" |
| 11:20-11:50 |
Toshihiko Fujimori (NIBB)
"Analysis of Cell Behaviors in Early Mouse Development" |
| 11:50-12:05 |
Naoki Hida (Univ. Tokyo) *Short talk from posters
“Real-Time Imaging of Dual Interaction of Smad Proteins in Xenopus laevis Embryo Using Multicolor Luciferases” |
| 12:05-13:10 |
Lunch |
| 13:10-13:40 |
Shigenori Nonaka (NIBB)
"Live Imaging of the Whole Mouse Embryo" |
| 13:40-13:55 |
Yu Imuta (RIKEN CDB) *Short talk from posters
“Establishment of Novel Knock-in Mice for Both Live Imaging and Genetic Lineage Tracing” |
| High-Throughput Imaging
|
| 13:55-14:25 |
Jan Ellenberg (EMBL Heidelberg)
"Phenotypic Profiling of the Human Genome by Time-Lapse Microscopy Reveals Genes with Functions in Cell Division, Survival or Migration" |
| 14:25-14:55 |
Gohta Goshima (Nagoya Univ.)
"Genome-Wide RNAi Screen Identifies Genes Required for Mitotic Spindle Formation in Animal Cells" |
| 14:55-15:15 |
Coffee break |
| 15:15-15:45 |
Philipp J. Keller (EMBL Heidelberg)
"The Virtual Embryo: Automated Reconstruction of Vertebrate Development" |
| 15:45-16:00 |
Peter Blattmann (EMBL Heidelberg) *Short talk from posters
“High Content Screening Microscopy Identifies Novel Proteins with a Putative Role in Cholesterol Metabolism and Homeostasis” |
| 16:00-16:15 |
Annelie Wuensche (EMBL Heidelberg) *Short talk from posters
“High-Throughput Confocal Time-Lapse Imaging in Living Cells” |
| 16:15-16:25 |
Short break |
| Quantitative Image Processing
|
| 16:25-16:55 |
Shuichi Onami (RIKEN ASI)
"Quantitative Cell Division Dynamics Analysis and Prediction of the Mechanism of Development in C. elegans" |
| 16:55-17:25 |
Takeharu Nagai (Hokkaido Univ.)
"Measurement of Diffusion Coefficient of Biomolecules by Fluorescence Decay After Photostimulation of Photoswichable Fluorescent Proteins in Living Cells" |
| 17:25-17:55 |
Matthias Weiss (DKFZ)
"Protein Motion in Living Cells - Anomalous Is Normal" |
| 17:55-18:10 |
Akatsuki Kimura (NIG) *Short talk from posters
“A Model for Cell-Size-Dependent Spindle Elongation in C. elegans Embryo” |
| 18:10-18:25 |
Yuki Tsukada (Nagoya Univ.) *Short talk from posters
“Edge Evolution Tracking: Morphodynamic Analysis for Time-Lapse Images” |
| 18:30-20:00 |
Poster Session |
| 20:00-21:00 |
NIBB Lab Tour |
| April 22 (Wednesday) |
| New Techniques in Advanced Microscopy
|
| 09:00-09:30 |
George Patterson (NIH)
"High Resolution Imaging of Protein Localization and Dynamics" |
| 09:30-10:00 |
Hiro-o Hamaguchi (Univ. Tokyo)
"Can Raman Spectroscopy Image Life?" |
| 10:00-10:30 |
John W. Sedat (UCSF)
"OMX, a New Microscope Platform for Increased Time and Spatial Resolution" |
| 10:30-10:50 |
Coffee break |
| 10:50-11:05 |
Satoshi Shimozono (Riken BSI) *Short talk from posters
“Diffusion of Large Molecules into Assembling Nuclei Revealed Using an Optical Highlighting Technique” |
| 11:05-11:20 |
Kenta Saito (Hokkaido Univ.) *Short talk from posters
“A Mercury Arc Lamp-Based Multi-Color Confocal Real Time Imaging System for Cellular Structure and Function” |
| 11:20-12:20 |
Poidum Discussion: Next Challenges in Functional Imaging Across Biological Scales (All speakers) |
| 12:20-12:30 |
Closing Remarks Jan Ellenberg (EMBL Heidelberg) |
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