Ding DQ, Okamasa K, Katou Y, Oya E, Nakayama JI, Chikashige Y, Shirahige K, Haraguchi T, Hiraoka Y.
Chromosome-associated RNA-protein complexes promote pairing of homologous chromosomes during meiosis in Schizosaccharomyces pombe.
Nature Commun. 10(1):5598. (2019)

Okamoto K, Tanaka Y, Ogasawara S, Obuse C, Nakayama JI, Yano H, Tsuneoka M.
KDM2A-dependent reduction of rRNA transcription on glucose starvation requires HP1 in cells, including triple-negative breast cancer cells.
Oncotarget 10(46):4743-4760. (2019)

Oya E, Durand-Dubief M, Cohen A, Maksimov V, Schurra C, Nakayama JI, Weisman R, Arcangioli B, Ekwall K.
Leo1 is essential for the dynamic regulation of heterochromatin and gene expression during cellular quiescence.
Epigenetics & Chromatin 12(1):45. (2019)

Hiragami-Hamada K, Nakayama JI.
Do the charges matter? - Balancing the charges of the chromodomain proteins on the nucleosome.
J Biochem 165(6):455-458. (2019)

Nishibuchi G, Machida S, Nakagawa R, Yoshimura Y, Hiragami-Hamada K, Abe Y, Kurumizaka H, Tagami H, Nakayama JI.
Mitotic phosphorylation of HP1α regulates its cell cycle-dependent chromatin binding.
J Biochem 165(5):433-446. (2018)

Hountondji C, Crechet JB, Tanaka M, Suzuki M, Nakayama JI, Aguida B, Bulygin K, Karpova G, Baouz S.
Ribosomal protein eL42 contributes to the catalytic activity of the yeast ribosome at the elongation step of translation.
Biochimie 158:20-33. (2018)

Bommi JR, Prasada Rao HD, Challa K, Higashide M, Shinmyozu K, Nakayama JI, Shinohara M, Shinohara A.
Meiosis-specific cohesin component, Rec8, promotes the localization of Mps3 SUN domain protein on the nuclear envelope.
Genes Cells 24(1):94-106. (2018)

Maksimov V, Oya E, Tanaka M, Kawaguchi T, Hachisuka A, Ekwall K, Bjerling P, Nakayama J.
The binding of Chp2’s chromodomain to methylated H3K9 is essential for Chp2’s role in heterochromatin assembly in fission yeast.
PLOS ONE 13(8):e0201101 (2018)

Nakayama N, Sakashita G, Nariai Y, Kato H, Sinmyozu K, Nakayama JI, Kyo S, Urano T, Nakayama K.
Cancer-related transcription regulator protein NAC1 forms a protein complex with CARM1 for ovarian cancer progression.
Oncotarget 9(47):28408-28420 (2018)

Okazaki K, Kato H, Iida T, Shinmyozu K, Nakayama JI, Murakami Y, Urano T.
RNAi-dependent heterochromatin assembly in fission yeast Schizosaccharomyces pombe requires heat-shock molecular chaperones Hsp90 and Mas5.
Epigenetics Chromatin 11(1):26 (2018)

Machida S, Takizawa Y, Ishimaru M, Sugita Y, Sekine S, Nakayama JI, Wolf M, Kurumizaka H.
Structural basis of heterochromatin formation by human HP1.
Mol Cell 69(3):385-397 (2018)

Zafar F, Okita AK, Onaka AT, Su J, Katahira Y, Nakayama J, Takahashi TS, Nasukata H, Nakagawa T.
Regulation of mitotic recombination between DNA repeats in centromeres.
Nucleic Acid Res.(2017) in press.

Shirai A, Kawaguchi T, Shimojo H, Muramatsu D, Ishida-Yonetani M, Nishimura Y, Kimura H, Nakayama J, Shinkai Y.
Impact of nucleic acid and methylated H3K9 binding activities of Suv39h1 on its heterochromatin assembly.
Elife6. pii: e25317. (2017)

Kawaguchi T, Machida S, Kurumizaka H, Tagami H, Nakayama J.
Phosphorylation of CBX2 controls its nucleosome-binding specificity.
J Biochem. (2017) in press.

Eustache S, Créchet J-B, Bouceba T, Nakayama J, Tanaka M, Suzuki M, Woisard A, Tuffery P, Baouz S, Hountondji C.
A Functional Role for the Monomethylated Gln-51 and Lys-53 Residues of the 49GGQTK53 Motif of eL42 from Human 80S Ribosomes.
Open Biochem J. 11:8-26. (2017)

Mutazono M, Morita M, Tsukahara C, Chinen M, Nishioka S, Yumikake T, Dohke K, Sakamoto M, Ideue T, Nakayama J, Ishii K, Tani T.
The intron in centromeric noncoding RNA facilitates RNAi-mediated formation of heterochromatin.
PLoS Genet. 13(2):e1006606. (2017)

Kamata K, Shinmyozu K, Nakayama J, Hatashita M, Uchida H, Oki M.
Four domains of Ada1 form a heterochromatin boundary through different mechanisms.
Genes Cells. 21(10):1125-1136. (2016)

Mitsumori R, Shinmyozu K, Nakayama JI, Uchida H, Oki M.
Gic1 is a novel heterochromatin boundary protein in vivo.
Genes Genet Syst. 91(3):151-159. (2016)

Shimojo H, Kawaguchi A, Oda T, Hashiguchi N, Omori S, Moritsugu K, Kidera A, Hiragami-Hamada K, Nakayama JI, Sato M & Nishimura Y
Extended string-like binding of the phosphorylated HP1α N-terminal tail to the lysine 9-methylated histone H3 tail.
Sci Rep. 6:22527. (2016)

Nishibuchi G, Machida S, Osakabe A, Murakoshi H, Hiragami-Hamada K, Nakagawa R, Fischle W, Nishimura Y, Kurumizaka H, Tagami H, Nakayama JI.
N-terminal phosphorylation of HP1α increases its nucleosome-binding specificity.
Nucleic Acids Res. 42(20):12498-12511. (2014)

Nishibuchi G, Shibata Y, Hayakawa T, Hayakawa N, Ohtani Y, Shinmyozu K, Tagami H, Nakayama JI.
Physical and Functional Interactions between the Histone H3K4 Demethylase KDM5A and the Nucleosome Remodeling and Deacetylase (NuRD) Complex.
J Biol Chem. 289(42):28956-70. (2014)

Hountondji C, Bulygin K, Créchet JB, Woisard A, Tuffery P, Nakayama J, Frolova L, Nierhaus KH, Karpova G, Baouz S.
The CCA-end of P-tRNA Contacts Both the Human RPL36AL and the A-site Bound Translation Termination Factor eRF1 at the Peptidyl Transferase Center of the Human 80S Ribosome.
Open Biochem J. 8:52-67. (2014)

Fawcett JA, Iida T, Takuno S, Sugino RP, Kado T, Kugou K, Mura S, Kobayashi T, Ohta K, Nakayama J, Innan H.
Population Genomics of the Fission Yeast Schizosaccharomyces pombe.
PLoS One. 9(8):e104241 (2014)

Nishibuchi G and Nakayama JI.
Biochemical and structural properties of Heterochromatin Protein 1: understanding its role in chromatin assembly
J. Biochem 156(1):11-20 (2014)

Kato H, Okazaki K, Iida T, Nakayama J, Murakami Y, Urano T.
Spt6 prevents transcription-coupled loss of posttranslationally modified histone H3.
Sci Rep. 3:2186 (2013)

Yasunobu Mano, Tetsuya J. Kobayashi, Jun-ichi Nakayama, Hiroyuki Uchida, Masaya Oki
Correlation of Epigenetic Switching between Multiple Single Cell Visualization of Yeast Gene Expression Shows Heterochromatic Regions through Multiple Generations
PLOS Biology. (7):e1001601 (2013)

Kamata K, Hatanaka A, Goswami G, Shinmyozu K, Nakayama JI, Urano T, Hatashita M, Uchida H, Oki M.
C-terminus of the Sgf73 subunit of SAGA and SLIK is important for retention in the larger complex and for heterochromatin boundary function.
Genes cells. (9):823-37 (2013)

Kawakami, K., Hayashi, A., Nakayama, J., and Murakami, Y.
A novel RNAi protein, Dsh1, assembles RNAi machinery on chromatin to amplify heterochromatic siRNA.
Genes Dev. 26, 1811-1824 (2012)

Ishida, M., Shimojo, H., Hayashi, A., Kawaguchi, R., Ohtani, Y., Uegaki, K., Nishimura, Y. and Nakayama, J.
Intrinsic Nucleic Acid-Binding Activity of Chp1 Chromodomain Is Required for Heterochromatic Gene Silencing.
Mol. Cell. 47, 228-41 (2012)

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Shiomi, Y., Ishii, T., Hayashi, A., Shinmyozu, K., Nakayama, J., Sugasawa, K. and Nishitani, H.
Two different RFC proteins, Ctf18 and RFC1 separately control PCNA-CRL4Cdt2-mediated Cdt1 proteolysis during S phase and following UV-irradiation.
Mol Cell Biol. 32, 2279-88 (2012)

Hayashi, A., Ishida, M., Kawaguchi, R., Urano, T., Murakami, Y., and Nakayama, J.
Heterochromatin protein 1 homologue Swi6 acts in concert with Ers1 to regulate RNAi-directed heterochromatin assembly.
Proc Natl Acad Sci U S A. 109, 6159-64 (2012)

Hatanaka, A., Chen, B., Sun, J.Q., Mano, Y., Funakoshi, M., Kobayashi, H., Ju, Y., Mizutani, T., Shinmyozu, K., Nakayama, J., Miyamoto, K., Uchida, H. and Oki, M.
Fub1p, a novel protein isolated by boundary screening, binds the proteasome complex.
Genes & Genetic Systems. 86, 305-314 (2011)

Goto, D.B. and Nakayama, J.
RNA and epigenetic silencing: Insight from fission yeast.
Dev Growth Differ. 54, 129-141 (2011)

Kitano, E., Hayashi, A., Kanai, D., Shinmyozu, K. and Nakayama, J.
Roles of fission yeast Grc3 in ribosomal RNA processing and heterochromatic gene silencing.
J. Biol. Chem. 286, 15391-402 (2011)

Hiragami-Hamada, K., Shinmyozu, K., Hamada, D., Tatsu, Y., Uegaki, S., Fujiwara, S. and Nakayama, J.
N-terminal phosphorylation of HP1α promotes its chromatin binding.
Mol. Cell. Biol. 31, 1186-200 (2011)

Hayakawa, T. and Nakayama, J.
Physiological roles of Class I HDAC complex and Histone demethylase.
J. Biomed. Biotechnol. (2011)

Shirai, A., Sadaie, M., Shinmyozu, K., and Nakayama, J.
Methylation of ribosomal protein L42 regulates ribosomal function and stress-adapted cell growth.
J. Biol. Chem. 285, 22448-60. (2010)

Hayakawa, T., Zhang, F., Hayakawa, N., Ohtani, Y., Shinmyozu, K., Nakayama, J.*, and Andressen, PR.*
MRG15 directly binds to PALB2 and stimulates homology-directed repair of chromosomal breaks.
J. Cell Sci. 123, 1124-30. (*corresponding authors) (2010)

Shimada, Y., Fukuda, W., Akada, Y., Ishida, M., Nakayama, J., Imanaka, T., and Fujiwara S.
Property of cold inducible DEAD-box RNA helicase in hyperthermophilic archaea.
Biochem. Biophys. Res. Commun. 389, 622-7. (2009)

Yamada, Y., Fukuda, W., Hirooka, K., Hiromoto, T., Nakayama, J., Imanaka, T., Fukusaki, E., and Fujiwara, S.
Efficient in vitro synthesis of cis-polyisoprenes using a thermostable cis-prenyltransferase from a hyperthermophilic archaeon Thermococcus kodakaraensis.
J. Biotechnol. 143, 151-6. (2009)

Okada, S., Nagabuchi, M., Takamura, Y., Nakagawa, T., Shinmyozu, K., Nakayama, J., and Tanaka, K.
Reconstitution of Arabidopsis thaliana SUMO pathways in E. coli: functional evaluation of SUMO machinery proteins and mapping of SUMOylation sites by mass spectrometry.
Plant Cell Physiol. 50, 1049-61. (2009)

Shimada, A., Dohke, K., Sadaie, M., Shinmyozu, K., Nakayama, J., Urano, T., and Murakami, Y.
Phosphorylation of Swi6/HP1 regulates transcriptional gene silencing at heterochromatin.
Genes Dev. 23, 18-23 (2009)

Hayashi, M.T., Takahashi, T.S., Nakagawa, T., Nakayama, J., and Masukata, H.
The heterochromatin protein Swi6/HP1 activates replication origins at pericentromeres and silent mating-type locus.
Nat. Cell Biol. 11, 357-62 (2009)

Sadaie, M., Kawaguchi, R., Ohtani, Y., Arisaka, F., Tanaka, K., Shirahige, K., and Nakayama, JI.
Balance between distinct HP1 family proteins controls heterochromatin assembly in fission yeast.
Mol. Cell. Biol. 28, 6973-88 (2008)

Sawada, T., Nishiyama, C., Kishi, T., Sasazuki, T., Komazawa-Sakon, S., Xue, X., Piao, JH., Ogata, H., Nakayama, J., Taki, T., Hayashi, Y., Watanabe, M., Yagita, H., Okumura, K., and Nakano, H.
Fusion of One twenty-two to BSAC (Basic, SAP, and coiled-coil domain) results in aberrant upregulation of transcriptional activity.
J. Biol. Chem. 283, 26820-26828 (2008)

Iida, T., Nakayama, J.,and Moazed, D.
siRNA-Mediated Heterochromatin Establishment Requires HP1 and Is Associated with Antisense Transcription.
Mol. Cell. 31, 178-189 (2008)

Sadaie, M., Shinmyozu, K.,and Nakayama, J.
A conserved SET-domain methyltransferase, Set11, modifies ribosomal protein Rpl12 in fission yeast.
J. Biol. Chem. 283, 7185-7195 (2008)

Chikashige, Y., Tsutsumi, C., Okamasa, K., Yamane, M., Nakayama, J., Niwa, O., Haraguchi, T., and Hiraoka, Y.
Gene expression and distribution of Swi6 in partial aneuploids of the fission yeast Schizosaccharomyces pombe.
Cell Struct. Funct. 32, 149-161 (2008)

Fujiwara, S., Yamanaka, A., Yamada, Y., Hirooka, K., Higashibata, H., Fukuda, W., Nakayama, JI., Imanaka, T., and Fukusaki, EI.
Efficient synthesis of trans-polyisoprene compounds using two thermostable enzymes in an organic-aqueous dual-liquid phase system.
Biochem. Biophys. Res. Commun. 365, 118-123 (2008)

Hayakawa, T., Ohtani,Y., Hayakawa, N., Shinmyozu, K., Saito, M., Ishikawa, F., and Nakayama, J.
RBP2 is an MRG15 complex component and downregulates intragenic histone H3 lysine 4 methylation.
Genes Cells. 12, 811-826 (2007)

Takasaki, N., Kurokawa, D., Nakayama, R., Nakayama, JI., and Aizawa,S.
Acetylated YY1 regulates Otx2 expression in anterior neuroectoderm at two cis-sites 90 kb apart.
EMBO J. 26, 1649-1659 (2007)

Buker, S.M., Iida, T., Buhler, M., Villen, J., Gygi, S.P., Nakayama, J., and Moazed, D.
Two different Argonaute complexes are required for siRNA generation and heterochromatin assembly in fission yeast.
Nat. Struct. Mol. Biol. 14, 200-207 (2007)

Takasaki, T., Liu, Z., Habara, Y., Nishiwaki, K., Nakayama, J., Inoue, K., Sakamoto, H., and Strome, S.
MRG-1, an autosome-associated protein, silences X-linked genes and protects germline immortarity in C. elegans.
Development. 134,757-67 (2007)

Iida, T., Kawaguchi, R., and Nakayama, J.
Conserved ribonuclease, Eri1, negatively regulates heterochromatin assembly in fission yeast.
Curr. Biol. 16, 1459-1464 (2006)

Tsumura, A., Hayakawa, T., Kumaki, Y., Takebayashi, S., Sakaue, M., Matsuoka, C., Shimotohno, K., Ishikawa, F., Li, E., Ueda, H. R., Nakayama, J., and Okano, M.
Maintenance of self-renewal ability of mouse embryonic stem cells in the absence of DNA methyltransferases Dnmt1, Dnmt3a and Dnmt3b.
Genes Cells. 11, 805-814. (2006)

Nishijima, H., Nakayama,J., Yoshioka, T., Kusano, A., Nishitani, H., Shibahara, K., and Nishimoto, T.
Nuclear RanGAP is required for the heterochromatin assembly and reciprocally regulated by histones and Clr4 histone methyltransferase in S. pombe.
Mol. Biol. Cell. 17, 2524-2536. (2006)

Sadaie, M., Iida, T., Urano, T., and Nakayama, J.
A chromodomain protein, Chp1, is required for the establishment of heterochromatin in fission yeast.
EMBO J. 23, 3825-3835. (2004)

Nakayama, J., Xiao, G., Noma, K., Malikzay, A., Bjerling, P., Ekwall, K., Kobayashi, R., and Grewal, S.I.S.
Alp13, an MRG family protein, is a component of fission yeast Clr6 histone deacetylase required for genome integrity.
EMBO J. 22, 2776-2787. (2003)

Tamaru, H., Zhang, X., McMillen, D., Singh, P., Nakayama, J., Grewal, S., Allis, D., Cheng, X., and Selker, E.U.
Trimethylation of histone H3 lysine-9 associated with methylated DNA in Neurospora crassa.
Nature Genet. 34, 75-79. (2003)

Nakagawa, H., Lee, J.-K., Hurwitz, J., Allshire, R.C., Nakayama, J., Grewal, S.I.S., Tanaka, K., and Murakami, Y.
Fission yeast CENP-B homologs nucleate centromeric heterochromatin by prompting heterochromatin-specific histone tail modifications.
Genes Dev. 16, 1766-1778. (2002)

Nakayama, J., and Ishikawa, F.
Stretch PCR assay.
Methods Mol. Biol. 191, 125-136. (2002)

Nakayama, J., Rice, J.C., Strahl, B.D., Allis, C.D., and Grewal, S.I.S.
Role of histone H3 lysine 9 methylation in epigenetic control of heterochromatin assembly.
Science. 292, 110-113. (2001)

Nakayama, J., Allshire, R.C., Klar, A.J.S., and Grewal, S.I.S
A role for DNA polymerase alpha in epigenetic control of transcriptional silencing in fission yeast.
EMBO J. 20, 2857-2866. (2001)

Nakayama, J., Klar, A.J.S., and Grewal, S.I.S.
A chromodomain protein, Swi6, performs imprinting functions in fission yeast during mitosis and miosis.
Cell. 101, 307-317. (2000)

Kawakami, Y., Kitamoto, M., Nakanishi, T., Yasui, W., Tahara, E., Nakayama, J., Ishikawa, F., Tahara, H., Ide, T., and Kajiyama, G.
Immuno-histochemical detection of human telomerase reverse transcriptase in human liver tissues.
Oncogene. 19, 3888-3893. (2000)

Poremba, C., Scheel, C., Hero, B., Christiansen, H., Schaefer, K.-L., Nakayama, J., Berthold, F., Juergens, H., Boecker, W., and Dockhorn-Dworniczak, B.
Telomerase activity and telomerase subunits gene expression patterns in neuroblastoma: A molecular and immunohistochemical study establishing prognostic tools for fresh-frozen and paraffin-embedded tissues.
J. Clin. Oncol. 18, 2582-2592. (2000)

Yuan, X., Ishibashi, S., Hatakeyama, S., Saito, M., Nakayama, J., Nikaido, R., Haruyama, T., Watanabe, Y., Iwata, H., Iida, M., Sugimura, H., Yamada, N., and Ishikawa, F.
Presence of telomeric G-strand tails in the telomerase catalytic subunit TERT knockout mice.
Genes Cells. 4, 563-572. (1999)

Yasui, W., Tahara, E., Tahara, H., Fujimoto, J., Naka, K., Nakayama, J., Ishikawa, F., Ide, T., and Tahara, E
Immunohistochemical detection of human telomerase reverse transcriptase in normal mucosa and precancerous lesions of the stomach.
Jpn. J. Cancer Res. 90, 589-595. (1999)

Tahara, H., Yasui, W., Tahara, E., Fujimoto, J., Ito, K., Tamai, K., Nakayama, J., Ishikawa, F., Tahara, E., and Ide, T.
Immuno-histochemical detection of human telomerase catalytic component, hTERT, in human colorectal tumor and non-tumor tissue sections.
Oncogene. 18, 1561-1567. (1999)

Yasui, W., Tahara, H., Tahara, E., Fujimoto, J., Nakayama, J., Ishikawa, F., Ide, T., and Tahara, E.
Expression of telomerase catalytic component, telomerase reverse transcriptase, in human gastric carcinomas.
Jpn. J. Cancer Res. 89, 1099-1103. (1998)

Nakayama, J., Tahara, H., Tahara, E., Saito, M., Ito, K., Nakamura, H., Nakanishi, T., Tahara, E., Ide, T., and Ishikawa, F.
Telomerase activation by hTRT in human normal fibroblast and hepatocellular carcinomas.
Nature Genet. 18, 65-68. (1998)

Saito, T., Matsuda, Y., Suzuki, T., Hayashi, A., Yuan, X., Saito, M., Nakayama, J., Hori, T., and Ishikawa, F.
Comparative gene mapping of the human and mouse TEP1 genes, which encode one protein component of telomerases.
Genomics. 46, 46-50. (1997)

Nakayama, J., Saito, M., Nakamura, H., Matsuura, A., and Ishikawa, F.
TLP1: A gene encoding a protein component of mammalian telomerase is a novel member of WD repeats family.
Cell. 88, 875-884. (1997)

Tatematsu, K., Nakayama, J., Danbara, M., Sato, H., Omine, M., and Ishikawa, F.
A novel quantitative 'stretch PCR assay', that detects a drastic increase in telomerase activity during the progression of myeloid leukemias.
Oncogene. 13, 2265-2274. (1996)

Kajita, Y., Nakayama, J., Aizawa, M., and Ishikawa, F.
The UUAG-specific RNA Binding Protein, hnRNP D0: Common Modular Structure and Binding Properties of 2xRBD-Gly Family.
J. Biol. Chem. 270, 22167-22175. (1995)