  NATIONAL INSITUTE FOR BASIC BIOLOGY

National Institute for Basic Biology

CENTER FOR ANALYTICAL INSTRUMENTS

(Managed by NIBB)

Head of Facility:
Tetsuo Yamamori

Technical Staffs:
Akio Murakami
Sonoko Ohsawa
Yumiko Makino
Takeshi Mizutani
Hatsumi Moribe (Apr. 21, 1997 -)

The Center, equipped with various types of instruments, serves for general use and supports biochemical structural analyses of biomolecules of proteins, nucleic acids and lipids. Amino acid sequence analysis, amino acid analysis, and chemical syntheses of peptides and DNA/RNA are carried out to support researchers in NIBB and NIPS. In April 1997, the Center started new services; site-specific modification of synthetic peptides (biotinylation and fluorophore labeling) and mass spectrometric analysis of peptides and proteins. Newly installed instruments in 1997 are Biomolecular Interaction Analysis System, Flow Cytometer, FT-IR Spectrophotometer and Microplate Luminometer. Some of instruments of the Center are opened to researchers outside the Institute. Representative instruments are listed below.

Protein Sequencers (ABI 492, ABI 473A) Amino Acid Analyzer (Hitachi L8500A) Peptide Synthesizers (ABI 433A, ABI 432A) Plasmid Isolation System (Kurabo PI-100·) DNA Sequencers (ABI 373S, ABI 310) DNA/RNA Synthesizers (ABI 394, ABI 392) Thermal Cyclers (Perkin Elmer PJ-1000, Takara TP-300) Integrated Thermal Cycler (ABI CATALYST Turbo 800) Gas Chromatograph (Shimadzu GC-14APF-SC) Glycoprotein Analysis System (Takara Glyco-Tag) High Performance Liquid Chromatographs (Shimadzu LC-10AD, Shimadzu LC-6AD) Integrated Micropurification System (Pharmacia SMART) Flow Cytometer (Coulter EPICS XL) NMR Spectrometer (Bruker AMX-360wb) EPR Spectrometer (Bruker ER-200D) GC/Mass Spectrometer (JEOL DX-300) Inductively Coupled Plasma Atomic Emission Spectrometer (Seiko SPS1200A) Spectrofluorometers (Hitachi 850, Simadzu RF-5000) Spectrophotometers (Hitachi 330, Hitachi 557, Varian Cary 5G, Perkin Elmer Lambda-Bio) Microplate Luminometer (Berthold MicroLumat LB 96P) Time-resolved Fluorescence Microplate Reader (Pharmacia DELFIA Research) Microplate Readers (Corona MTP-120, MTP-100F) Spectropolarimeter (JASCO J-40S) FT-IR Spectrophotometer (Horiba FT-730) Laser Raman Spectrophotometer (JASCO R-800) Biomolecular Interaction Analysis Systems (Pharmacia BIAcore 2000, Affinity Sensors IAsys) Bio Imaging Analyzer (Fujifilm BAS2000) Electrophoresis Imaging Systems (PDI Discovery Series, BIOIMAGE) Microscopes (Carl Zeiss Axiophot, Axiovert) Microscope Photometer (Carl Zeiss MPM 03-FL)

Figure 1.
Amino acid sequence analysis of proteins by an automated protein sequencer.

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Last Modified: 12:00, May 28, 1998