-文部科学省科学研究費補助金「特定領域研究」細胞核ダイナミクス　大山　隆-

細胞核ダイナミクスにおけるゲノムの機能的収納機構の解明

研究代表者

大山　隆（研究代表者）＜早稲田大学教育・総合科学学術院・生物学専修・分子遺伝学研究室・教授＞〒169-8050 東京都新宿区西早稲田1-6-1 TEL: 03-5286-1520 FAX: 03-3207-9694 E-mail: ohyama@waseda.jp ・主な研究内容・研究室メンバー・最近の研究成果・総説・解説等・最近の著書

主な研究内容

・ゲノムDNAの高次構造に印された遺伝情報の解読
・ゲノムDNAの機械的特性に印された遺伝情報の解読
・機能的クロマチンの構築機構
・クロマチン工学の開拓
・DNA・ヌクレオソームの自己集合に関する研究
・植物の環境応答とエピジェネティックな遺伝子発現制御機構に関する研究

研究室メンバー

・棚瀬潤一（D2）
・宇田川紘司（M1）
・木村元（M1）
・小平玲菜（M1）
・三浦真梨子（M1）
・浅野士郎（B4）
・伊藤美月（B4）
・瓜生寿子（B4）
・景山大（B4）
・西村恵美（B4）
・古屋美香（B4）
・山本拓弥（B4）

最近の研究成果

Inoue, S., Sugiyama, S., Travers, A.A. and Ohyama, T. Self-assembly of double-stranded DNA molecules at nanomolar concentrations. Biochemistry, 46, 164-171 (2007).

Kamiya, H., Fukunaga, S., Ohyama, T. and Harashima, H. The location of the left-handedly curved DNA sequence affects exogenous DNA expression in vivo. Arch. Biochem. Biophys., 461, 7-12 (2007).

Sumida, N., Nishikawa, J., Kishi, H., Amano, M., Furuya, T., Sonobe, H. and Ohyama, T. A designed curved DNA segment that is a remarkable activator of eukaryotic transcription. FEBS J. 273, 5691-5702 (2006).

Matsugami, A., Tani, K., Ouhashi, K., Uesugi, S., Morita, M., Ohyama, T. and Katahira, M. Structural property of DNA that migrates faster in gel electrophoresis, as deduced by CD spectroscopy. Nucleosides, Nucleotides and Nucleic Acids 25, 417-425 (2006).

Okabe, T., Iwakiri, Y., Mori, H., Ogawa, T. and Ohyama, T. An S-like ribonuclease gene is used to generate a trap-leaf enzyme in the carnivorous plant Drosera adelae. FEBS Lett. 579, 5729-5733 (2005).

Fukue, Y., Sumida, N., Tanase, J. and Ohyama, T. A highly distinctive mechanical property found in the majority of human promoters and its transcriptional relevance. Nucl. Acids Res. 33, 3821-3827 (2005).

Fukue, Y., Sumida, N., Nishikawa, J. and Ohyama, T. Core promoter elements of eukaryotic genes have a highly distinctive mechanical property. Nucl. Acids Res. 32, 5834-5840 (2004).

Nishikawa, J., Amano, M., Fukue, Y., Tanaka, S., Kishi, H., Hirota, Y., Yoda, K. and Ohyama, T. Left-handedly curved DNA regulates accessibility to cis-DNA elements in chromatin. Nucl. Acids Res. 31, 6651-6662 (2003).

Asayama, M., Kato, H., Shibata, J.,Shirai, M. and Ohyama, T. The curved DNA structure in the 5'-upstream region of the light-responsive genes: its universality, binding factors and function for cyanobacterial psbA transcription. Nucl. Acids Res. 30, 4658-4666 (2002).

Tagashira, H., Morita, M. and Ohyama, T. Electrophoretic mobility shift of restriction fragments caused by base pairing between overhangs. Biochemistry 41, 12217-12223 (2002).

Kusakabe, T., Sugimoto, Y., Maeda, T., Miyano, M., Nishikawa, J., Tone, S., Kawaguchi, Y., Koga, K. and Ohyama, T. Linearization and integration of DNA into cells preferentially occurs at intrinsically curved regions from human LINE-1 repetitive element. Gene 274, 271-281 (2001).

総説・解説等

Ohyama T.
Regulation of chromatin structure by curved DNA: how activator binding sites become accessible. In Nagata, K. and Takeyasu, K. (eds.), Nuclear Dynamics-Molecular Biology and Visualization of the Nucleus. Springer-Verlag (Tokyo), 227-238 (2007).

Ohyama, T., Sumida, N., Tanase, J., Inoue, S. and Okabe T.
Genetic information carried in DNA conformation and properties, In Kiyama, R. and Shimizu, M. (eds), DNA Structure, Chromatin and Gene Expression. Transworld Research Network (Kerala), 71-84 (2006).

Asayama, M. and Ohyama, T.
Curved DNA and prokaryotic promoters: a mechanism for activation of transcription. In: Ohyama, T. (ed.), DNA Conformation and Transcription. Springer (New York) and Landes Bioscience (Texas), 37-51 (2005).

Ohyama, T.
Curved DNA and Transcription in eukaryotes. In: Ohyama, T. (ed.), DNA Conformation and Transcription. Springer (New York) and Landes Bioscience (Texas), 66-74 (2005).

Ohyama, T.
The role of unusual DNA structures in chromatin organization for transcription. In: Ohyama, T. (ed.), DNA Conformation and Transcription. Springer (New York) and Landes Bioscience (Texas), 177-188 (2005).

Ohyama, T.
Intrinsic DNA bends: an organizer of local chromatin structure for transcription. BioEssays 23, 708-715 (2001).

最近の著書

Nagata, K. and Takeyasu, K. (eds.)「Nuclear Dynamics-Molecular Biology and Visualization of the Nucleus」（分担執筆）Springer-Verlag (Tokyo), (2007).

東中川徹、大山隆、清水光弘（共編）「ベーシックマスター分子生物学」（分担執筆）オーム社、（2006）.

Kiyama, R. and Shimizu, M. (eds), 「DNA Structure, Chromatin and Gene Expression」（分担執筆）Transworld Research Network (Kerala), (2006).

Ohyama, T. (ed.)「DNA Conformation and Transcription」(分担執筆) Springer (New York) and Landes Bioscience (Texas), (2005).

＊執筆部分の内容は総説欄に記載。