RNAi装置に関与するDicer遺伝子の条件的ノックアウト細胞を樹立し、Dicerが高等動物細胞においてもヘテロクロマチンの形成に重要な働きを担っていることを示した。また、キネトコア構築に関わる蛋白質同定の解析も行い、複数の構成的セントロメア蛋白質群によるキネトコア構築に関わる新知見を得た。

発表論文リスト：

1. Hori, T., Amano, M., Suzuki, A., Backer, C., Welburn, J.P., Dong, Y., McEwen, B.F., Shang, W.H., Suzuki, E., Okawa, K., Cheeseman, I.M., and Fukagawa, T. CCAN makes multiple contacts with centromeric DNA and provides distinct pathways to the outer kinetochore Cell 135: 1039-1052 (2008)
2. Hori, T., Okada, M., Maenaka, K. and Fukagawa, T. CENP-O-class proteins form a stable complex and are required for proper kinetochore function. Mol. Biol. Cell, 19: 843-854, (2008)
3. Cheeseman, I.M., Hori, T., Fukagawa, T. and Desai, A. KNL1 and CENP-H/I/K complex coordinately direct kinetochore assembly in vertebrates. Mol. Biol. Cell, 19: 587-594, (2008)
4. Fukagawa, T. The kinetochore and spindle checkpoint in vertebrate cells. Frontiers in Biosci., 13: 2705-2713, (2008)
5. Kwon, M.-S., Hori, T., Okada, M. and Fukagawa, T. CENP-C is involved in chromosome segregation, mitotic checkpoint function and kinetochore assembly. Mol. Biol. Cell, 18: 2155-2168, (2007)
6. Zuccolo, M., Alves, A., Galy, V., Bolhy, S., Formstecher, E., Racine, V., Sibarita, J.-B., Fukagawa, T., Shiekhattar, R., Yen, T. and Doye, V. The human Nup107-160 nuclear pore subcomplex contributes to proper kinetochore functions. EMBO J., 26: 1853-1864, (2007)
7. Okada, M. and Fukagawa, T. Purification of a protein complex that associates with chromatin. Nature Protocols, DOI: 10.1038/nprot.2006.417, (2006)
8. Kimura, H., Takizawa, N., Allemand, E., Hori, T., Iborra, F. J., Nozaki, N., Muraki, M., Hagiwara, M., Krainer, A.R., Fukagawa, T. and Okawa, K. A novel histone-exchange factor, protein phosphatase 2Cγ, mediates the exchange and dephosphorylation of H2A/H2B. J. Cell Biol., 175: 389-400, (2006)
9. Okada, M., Cheeseman, I.M., Hori, T., Okawa, K., McLeod, I.X., Yates III, J.R. Desai, A. and Fukagawa.T. The CENP-H-I complex is required for the efficient incorporation of newly synthesized CENP-A into centromeres. Nature Cell Biol., 8: 446-457 (2006)
10. Kline, S., Cheeseman, I.M., Hori, T., Fukagawa, T. and Desai. A. The Human Mis12 Complex is Required for Kinetochore Assembly and Proper Chromosome Segregation. J. Cell Biol., 173: 9-17 (2006)
11. Sanematsu, F., Takami, Y., Barman, H.K., Fukagawa, T., Ono, T., Shibahara, K. and Nakayama, T. Asf1 is required for viability and chromatin assembly during DNA replication in vertebrate cells. J. Biol. Chem., 281: 13817-13827 (2006)
12. Yamauchi, A., Ichimiya, T., Inoue, K., Taguchi, Y., Matsunaga, N., Koyanagi, S., Fukagawa, T., Aramaki, H., Higuchi, S. and Ohdo, S. Cell cycle dependent pharmacology of methotrexate in HL-60. J. Pharm. Sci., 99: 335-341 (2005)
13. Minoshima, Y., Hori, T., Okada, M., Kimura, H., Haraguchi, T., Hiraoka, Y., Bao, Y.-C., Kawashima, T., Kitamura, T. and Fukagawa, T. The Constitutive Centromere Component CENP-50 is Required for Recovery from Spindle damage. Mol. Cell. Biol., 25: 10315-10328 (2005)
14. Regnier, V., Vagnarelli, P., Fukagawa, T., Zejal, T., Burns, E., Trouche, D., Earnshaw, W. and Brown, W. CENP-A depletion leads to impaired mitotic checkpoint maintenace in vertebrate cells. Mol. Cell. Biol., 25: 3967-3981 (2005)
15. Mikami, Y., Hori, T., Kimura, H. and Fukagawa, T. Functional region of CENP-H interacts with Nuf2 complex, which functions as a connector between the inner and outer kinetochores. Mol. Cell. Biol., 25: 1958-1970 (2005)
16. Fukagawa, T., Nogami, M., Yoshikawa, M., Ikeno, M., Okazaki, T., Takami, Y., Nakayama, T. and Oshimura, M. Dicer is essential for formetion of the heterochromatin structure in vertebrate cells. Nature Cell Biol., 6: 784-791 & Cover (2004)
17. Fukagawa, T., Centromere DNA, proteins and kinetochore assembly in vertebrate cells. Chromosome Res., 12: 557-567 (2004)
18. Fukagawa, T., Assembly of kinetochores in vertebrate cells. Exp. Cell Res., 296: 21-27 (2004)