3.5 Gravitropic response on caulonemata
Tomomichi Fujita
(1) Materials
a. BCDAT agar plates supplemented with sucrose
(2%) or glucose (0.5%).
b. Boxes to keep the plates in the dark
(2) Protocol
1. Inoculate
protonemata on the agar plate and culture for 1-2 weeks under the light
conditions.
2. After the culture
in step 1, stick the plate on the bottom of a box. It is a good idea to wrap
the plate with aluminum foil to completely prevent light. Stand the box
vertically and culture for 1-3 weeks.
(3) Results
You can easily observe the negative gravitropic
response of protonemata, Similar gravitropic responses are also observed in Funaria
hygrometrica and Ceratodon purpureus in the same conditions.
Figure.
Protonemata were precultured at 25℃ for 10 days under light conditions, then placed
vertically at 25℃ for 21 days under dark
conditions.