2.4  Sporangium collection and storage

Yuji Hiwatashi



Spore preservation is the most stable method. A mature sporangium is harvested, dried and stored at 4˚C. A sporangium contains thousands of spores. We have confirmed that spores could be stored for at least 4 years under this condition, and should be stable longer.



1. Cultivate gametophores at 15˚C under the short-day condition (8L16D) and induce sporophyte .

2. After development of a brown sporophyte at the apex of a gametophore, cut seta of a sporophyte with a scalpel and a forceps, and separate sporangium from a gametophore. Do not cut green sporangium that has not yet been matured. 

Cut here



3. Add sporangia into a micro tube and air-dry sporangia for a couple of days.

4. Store at 4˚C. 



Gametophores with sporophytes





[Key points]

Collect mature brown sporangia. Dot not collect immature green sporangia.