Introduction
Spore
preservation is the most stable method. A mature sporangium is harvested, dried
and stored at 4˚C. A sporangium contains thousands of spores. We have
confirmed that spores could be stored for at least 4 years under this
condition, and should be stable longer.
1. Cultivate
gametophores at 15˚C under the short-day condition (8L16D) and induce
sporophyte .
2. After
development of a brown sporophyte at the apex of a gametophore, cut seta of a
sporophyte with a scalpel and a forceps, and separate sporangium from a
gametophore. Do not cut green sporangium that has not yet been matured.
Cut here
3. Add sporangia
into a micro tube and air-dry sporangia for a couple of days.
4. Store at
4˚C.
[Materials]
・ Gametophores with
sporophytes
・ Forceps
・ Scalpel
・ Microtube
[Key
points]
・ Collect mature brown
sporangia. Dot not collect immature green sporangia.