THE CENTER FOR ANALYTICAL INSTRUMENTS (managed by NIBB)

The Center serves for amino acid sequence analysis, and chemical syntheses of peptides and nucleotids to support researchers in NIBB and NIPS. Newly installed instrument in 2001 is MALDI/TOF-MS. Instruments of the Center can be used by researchers outside the Institute upon proposal.

Figure 1. MALDI/TOF-MS.		Figure 2. Biomek 2000 Laboratory Automation System.

Representative instruments are listed below.

• Protein Sequencers (ABI Procise 494, 473A)
• Amino Acid Analyzer (Hitachi L8500A)
• Peptide Synthesizers (ABI 433A, 432A)
• Plasmid Isolation Systems (Kurabo PI-100S, PI-50)
• Automatic Nucleic Acid Isolation System (Kurabo NA-2000)
• DNA Sequencers (ABI 377, 373S, 310)
• DNA/RNA Synthesizers (ABI 394, 392)
• Thermal Cyclers (Perkin Elmer PJ-9600, Takara TP-300)
• Integrated Thermal Cyclers (ABI CATALYST Turbo 800)
• Particle Delivery System (Bio-Rad BiolisticPDS-1000/He)
• Gas Chromatograph (Shimadzu GC-14APF-SC)
• Glycoprotein Analysis System (Takara Glyco-Tag)
• High Performance Liquid Chromatographs (Shimadzu LC-10AD, LC-6AD, Waters 600E)
• Integrated Micropurification System (Pharmacia SMART)
• Flow Cytometer (Coulter EPICS XL)
• Biomolecular Interaction Analysis Systems (Pharmacia BIACORE 2000, Affinity Sensors IAsys)
• Laboratory Automation System (Beckman Coulter Biomek 2000)
• NMR Spectrometer (Bruker AMX-360wb)
• EPR Spectrometer (Bruker ER-200D)
• GC/Mass Spectrometer (JEOL DX-300)
• MALDI/TOF-MS (Bruker Daltonics REFLEX III)
• Inductively Coupled Plasma Atomic Emission Spectrometer (Seiko SPS1200A)
• Spectrofluorometers (Hitachi 850, Simadzu RF-5000)
• Spectrophotometers (Hitachi 330, 557, Varian Cary 5G, Perkin Elmer Lambda-Bio)
• Microplate Luminometer (Berthold MicroLumat LB 96P)
• Time-resolved Fluorescence Microplate Reader (Pharmacia DELFIA Research)
• Microplate Readers (Corona MTP-120, MTP-100F)
• Spectropolarimeter (JASCO J-40S)
• FT-IR Spectrophotometer (Horiba FT-730)
• Laser Raman Spectrophotometer (JASCO R-800)
• Bio Imaging Analyzers (Fujifilm BAS2000)
• Fluorescence Bio Imaging Analyzer (Takara FMBIO)
• Electrophoresis Imaging Systems (BIOIMAGE)
• Microscopes (Carl Zeiss Axiophot, Axiovert)