文部科学省科学研究費特定領域研究「性分化機構の解明」 *

研究成果

長濱 嘉孝 NAGAHAMA, Yoshitaka
自然科学研究機構 基礎生物学研究所 
研究項目A01 「性分化の分子基盤の解析」
研究課題 魚類における性決定と生殖腺の性分化の分子メカニズム

2006年発表論文

Suppression of steroidogenic enzyme expression during androgen-induced sex reversal in Nile tilapia (Oreochromis niloticus). R.K. Bhandari, M. Nakamura, T. Kobayashi, Y. Nagahama Gen. Comp. Endocrinol. 145, 20-24, 2006

Forebrain gonadotropin-releasing hormone neuronal development: insights from transgenic medaka and the relevance to X-linked Kallmann syndrome. K. Okubo, F. Sakai, E.L. Lau, G. Yoshizaki, Y. Takeuchi, K. Naruse, K. Aida, Y. Nagahama Endocrinology 147, 1076-1084, 2006

Promoter activity and chromosomal location of the Rana rugosa P450 aromatase (CYP19) gene. Y. Oshima, T. Kato, D.S. Wang, T. Murakami, M. Matsuda, Y. Nagahama, M. Nakamura Zool. Sci. 23, 79-85, 2006

Cloning and identification of a membrane progestin receptor in goldfish ovaries and evidence it is an intermediary in oocyte meiotic maturation. M. Tokumoto, Y. Nagahama, P. Thomas, T. Tokumoto Gen. Comp. Endocrinol. 145, 101-108, 2006

Molecular cloning, gene expression and characterization of the third estrogen receptor of the Nile tilapia, Oreochromis niloticus. D.S. Wang, B. Senthilkumaran, C.C. Sudhakumari, F. Sakai, M. Matsuda, T. Kobayashi, M. Yoshikuni, Y. Nagahama Fish Physiol.Biochem. (in press)

Molecular mechanisms of sex determination and gonadal sex differentiation in fish. Y. Nagahama Fish Physiol. Biochem. (in press)

Liver receptor homologue-1 activates brain aromatase promoter of medaka, Oryzias latipes. Y. Ohmuro-Matsuyama, K. Okubo, M. Matsuda, S. Ijiri, D.S. Wang, G.J. Guan, T. Suzuki, M. Matsuyama, K. Morohashi, Y. Nagahama Mol. Reprod. Develop. (in press)

Ontogenic expression patterns of several nuclear receptors and cytochrome P450 aromatases in brain and gonads of the Nile tilapia Oreochromis niloticus suggests their involvement in sex differentiation. C.C. Sudhakumari, B. Senthilkumaran, T. Kobayashi, H. Kajiura-Kobayashi, D.S. Wang, M. Yoshikuni, Y. Nagahama Fish Physiol. Biochem. (in press)

Roles of three transcription factors of the NR5A subfamily on estradiol 17β and gonadotropin-induced changes in Cyp19a1 expression in the ovarian follicles of the medaka, Oryzias latipes Y. Ohmuro-Matsuyama, Y. Shibata, R. Chaube, M. Yoshikuni, K. Okubo, Y. Nagahama, S. Ijiri Mol. Reprod. Develop. (in press)

2005年発表論文

Zhou, L.Y., Wang, D.S., Senthilkumaran, S., Yoshikuni, M., Shibata, Y., Kobayashi, T., Sudhakumari, C.C. and Nagahama, Y. (2005). Cloning, expression and characterization of three types of 17b-hydroxysteroid dehydrogenases from the Nile tilapia, Oreochromis niloticus. J. Mol. Endocrinol.35, 103-116.doi:10.1677/jme.1.01801

Kobayashi, Y., Sunobe, T., Kobayashi, T., Nagahama, Y. and Nakamura, M. (2005). Gonadal structure of the serial-sex changing fish Trimma Okinawa. Dev. Growth Differ. 47, 7-13.doi:10.1111/j.1440-169x.2004.00774.x

Nakamoto, M., Suzuki, A., Matsuda, M., Nagahama, Y. and Shibata, N. (2005). Testicular type Sox9 is not involved in sex determination, but might be in the development of testicular structures in the medaka, Oryzias latipes. Biochem. Biophys. Res. Commun.333, 729-736. doi:10.1016/j.bbrc.2005.05.158.

2004年発表論文

Kobayashi, T., Kobayashi, H. and Nagahama, Y. (2004). Two DM domain genes, DMY and DMRT1, involved in testicular differentiation and development in the medaka, Oryzias latipes. Dev. Dyn. 231, 518-526.

Kobayashi, Y., Kobayashi, T., Nakamura, M., Sunobe, T., Morrey, C.E., Suzuki, N. and Nagahama, Y. (2004). Characterization of two types of cytochrome P450 aromatase in the serial-sex changing gobiid fish, Trimma okinawae. Zool. Sci. 21, 417-425.

Senthilkumaran, B. and Nagahama, Y. (2004). A shift in steroidogenesis occurring in ovarian follicles prior to oocyte maturation. Mol. Cell. Endocrinol. 215, 11-18.

Suzuki, A., Tanama, M., Nagahama, Y. and Shibata, N. (2004). Expression of aromatase mRNA and effects of aromatase inhibitor during ovarian development in the medaka, Oryzias latipes. J. Exp. Zool. 301A, 266-273.

Wang, D.S., Zhou, L.Y., Kobayashi, T. and Nagahama, Y. (2004). Molecular cloning and gene expression of Foxl2 in the Nile tilapia, Oreochromis niloticus. Biochem. Biophys. Res. Commun. 320, 83-89.

長濱嘉孝、小林 亨、松田 学(2004)。魚類の性決定と生殖腺の性分化/性転換。動物の性はどのように決まるか?:性決定機構の共通性と多様性。蛋白質 核酸 酵素。49、116-123.

長濱嘉孝(2004)。魚類生殖系に及ぼす影響のメカニズム。内分泌撹乱物質‐どこまでわかったか(8)。現代化学。2004年8月、40-45.