文部科学省科学研究費特定領域研究「性分化機構の解明」 *

研究成果

松田 洋一 MATSUDA, Youichi
北海道大学創成科学共同研究機構  ゲノムダイナミクス研究部門
研究項目A01 「性分化の分子基盤の解析」
研究課題 性決定機構が未解明な動物種における性染色体の構造と性決定関連遺伝子群の解析

Evidence for different origin of sex chromosomes in snakes, birds, and mammals and step-wise differentiation of snake sex chromosomes. Matsubara K, Tarui H, Toriba M, Yamada K, Nishida-Umehara C, Agata K, Matsuda Y.PNAS 103(48):18190-18195

研究内容概説ーヒト、ニワトリ、ヘビの遺伝的類縁関係を調べる目的で、109の機能遺伝子からな るシマヘビの染色体地図を作製し、Z染色体に連鎖する11の機能遺伝子を見つけ た。これらの遺伝子が全てニワトリとヒトの常染色体に存在することから、ヘビ、鳥 類、哺乳類の性染色体が共通祖先のそれぞれ異なる常染色体に由来することを明らか にした。さらにこれらの遺伝子を旧蛇類のビルマニシキヘビと新蛇類のハブの ZW 染 色体上にマッピングした結果、ヘビ類がもつ性染色体は、ヘビ類の共通の祖先におい て性決定機能をもった性染色体が誕生した後、原始的なボア・ニシキヘビの仲間から 毒ヘビの仲間へ進化する過程で性特異的な染色体に構造変化と欠失が生じ矮小化とヘ テロクロマチン化が進んでいったことが推定された。


2006年発表論文

New families of site-specific repetitive DNA sequences that comprise constitutive heterochromatin of the Syrian hamster (Mesocricetus auratus, Cricetinae, Rodentia). K. Yamada, E. Kamimura, M. Kondo, K. Tsuchiya, C. Nishida-Umehara, Y. Matsuda. Chromosoma 115, 36-49, 2006

X accumulation of LINE-1 retrotransposons in Tokudaia osimensis, a spiny rat with the karyotype XO. L.A. Scott, A. Kuroiwa, Y. Matsuda, H.A. Wichman. Cytogenet. Genome Res. 112, 261-269, 2006

Promoter activity and chromosomal location of the Rana rugosa P450 aromatase (P450arom) gene. Y. Oshima, T. Kato, D. Wang, T. Murakami, Y. Matsuda,Y. Nagahama, M. Nakamura. Zool. Sci. 23, 79-85, 2006.

cDNA-based gene mapping and GC3 profiling in soft-shelled turtle suggests chromosome size-dependent GC-bias shared by sauropsids. S. Kuraku, J. Ishijima, C. Nishida-Umehara, K. Agata, S. Kuratani, Y. Matsuda. Chromosome Res. 14, 187-202, 2006

A novel family of repetitive DNA sequences amplified site-specifically on the W chromosomes in Neognathous birds. K. Yamada, C. Nishida-Umehara, J. Ishijima, T. Murakami, M. Shibusawa, K. Tsuchiya, M. Tsudzuki, Y. Matsuda. Chromosome Res. (in press)

2005年発表論文

Molecular and cytogenetic characterization of site-specific repetitive DNA sequences in the Chinese soft-shelled turtle (Pelodiscus sinensis, Trionychidae). K. Yamada, C. Nishida-Umehara, Y. Matsuda Chromosome Res. 13, 33-46, 2005

Highly conserved linkage homology between birds and turtles: birds and turtle chromosomes are precise counterparts of each other. Y. Matsuda, C. Nishida-Umehara, H. Tarui, A. Kuroiwa, K. Yamada, T. Isobe, J. Ando, A. Fujiwara, Y. Hirao, O. Nishimura, J. Ishijima, A. Hayashi, T. Saito, T. Murakami, Y. Murakami, S. Kuratani, K. Agata. Chromosome Res. 13, 601-615, 2005

Isolation and characterization of novel testis-specific genes from mouse pachytene spermatocyte-enriched cDNA library. T. Ijiri, T. Nagase, Y. Matsuda Reprod. Med. Biol. 4, 231-237, 2005

2004年発表論文

Kuramochi-Miyagawa, S., Kimura, T., Ijiri, T., Asada, N., Fujita, Y., Ikawa, M., Isobe, T., Iwai, N., Okabe, M., Deng, W., Lin, H., Matsuda, Y. and Nakano, T.: mili, a mammalian member of piwi family gene, is essential for spermatogenesis. Development 131:839-849 (2004).

Masabanda, J. S., Burt, D. W., O'Brien, P. C., Vignal, A., Fillon, V., Walsh, P. S., Cox, H., Tempest, H. G., Smith, J., Habermann, F., Schmid, M., Matsuda, Y., Ferguson-Smith, M.A., Crooijmans, R. P., Groenen, M. A. and Griffin, D. K.: Molecular cytogenetic definition of the chicken genome: the first complete avian karyotype. Genetics 166:1367-73 (2004).

Shibusawa, M., Nishibori, M., Nishida-Umehara, C., Tsudzuki, M., Masabanda, J., Griffin, D. K. and Matsuda, Y.: Karyotypic evolution in the Galliformes: An examination of the process of karyotypic evolution by comparison of the molecular cytogenetic findings with the molecular phylogeny. Cytogenetic and Genome Research 106:111-119 (2004).

Matsubara, K., Nishida-Umehara, C., Tsuchiya, K., Nukaya, D. and Matsuda, Y.: Karyotypic evolution inferred from comparative FISH analyses in Apodemus (Muridae, Rodentia). Chromosome Research 12:383-395 (2004).