Genetics and Imaging of Medaka and Zebrafish

Application has been closed.

Participation: Open application with selection (maximum 16 participants)

Contents:
“Genome Editing”
Gene knock-in using the CRISPR/Cas9 system in zebrafish
by Shin-ichi HIGASHIJIMA (NIBB)
Gene knock-out using the CRISPR/Cas9 system in medaka
by Satoshi ANSAI (NIG) and Yusuke TAKEHANA (NIBB)

“PhiC31 Transgenesis”
Highly efficient transgenesis using the PhiC31 system in medaka
by Satoshi ANSAI (NIG)

“Behavioral Assay”
Optomotor response in medaka mutant
by Shoji FUKAMACHI (Japan Women’s Univ.) and Saori YOKOI (NIBB)

“Imaging”
Local gene induction with infrared laser-evoked gene operator (IR-LEGO) in medaka
by Yasuhiro KAMEI (NIBB)
in vivo imaging
by Shigenori NONAKA (NIBB), Sachihiro SUZUKI (OIST) and Shinya KOMOTO (EMBL)

“Strain Preservation”
Cryopreservation of sperm and artificial insemination in medaka
by Yusuke TAKEHANA and Kiyoshi NARUSE (NIBB)