{"id":84,"date":"2018-03-26T11:44:37","date_gmt":"2018-03-26T02:44:37","guid":{"rendered":"http:\/\/www.nibb.ac.jp\/imori\/main\/?page_id=84"},"modified":"2020-06-24T17:27:58","modified_gmt":"2020-06-24T08:27:58","slug":"protocols-japanese-or-english","status":"publish","type":"page","link":"http:\/\/www.nibb.ac.jp\/imori\/main\/?page_id=84","title":{"rendered":"Protocols (Japanese or English)"},"content":{"rendered":"<h3>Husbandry (Tottori method)<\/h3>\n<pre>\u3000\u3053\u306e\u30d7\u30ed\u30c8\u30b3\u30fc\u30eb\u306f\u5e83\u5cf6\u5927\u5b66\u4e21\u751f\u985e\u7814\u7a76\u30bb\u30f3\u30bf\u30fc\u5668\u5b98\u518d\u751f\u30e1\u30ab\u30cb\u30ba\u30e0\u7814\u7a76\u30b0\u30eb\u30fc\u30d7\u306e\u30e1\u30f3\u30d0\u30fc\u306b\u3088\u3063\u3066\u4f5c\u6210\u3055\u308c\u305f\u98fc\u80b2\u30de\u30cb\u30e5\u30a2\u30eb\u3067\u3059\u3002\u7121\u65ad\u5f15\u7528\u3001\u8ee2\u8f09\u3092\u7981\u3058\u307e\u3059\u3002\r\n\u3000\u306a\u304a\u3001\u672c\u30de\u30cb\u30e5\u30a2\u30eb\u306f\u5e83\u5cf6\u5927\u5b66\u304c\u904b\u7528\u3059\u308b\u516c\u5f0f\u30de\u30cb\u30e5\u30a2\u30eb\u3067\u306f\u3042\u308a\u307e\u305b\u3093\u3002<\/pre>\n<p><span style=\"color: #0000ff;\"><a href=\"http:\/\/www.nibb.ac.jp\/imori\/main\/wp-content\/uploads\/2018\/03\/0_\u8868\u7d1920180326.pdf\"><span style=\"color: #0000ff;\">0_\u8868\u7d19<\/span> ver.20180326<\/a><\/span><\/p>\n<p><span style=\"color: #0000ff;\"><a href=\"http:\/\/www.nibb.ac.jp\/imori\/main\/wp-content\/uploads\/2018\/03\/1_\u500b\u4f53\u306e\u5165\u624b\u65b9\u6cd520171016.pdf\"><span style=\"color: #0000ff;\">1_\u500b\u4f53\u306e\u5165\u624b\u65b9\u6cd5<\/span> ver.20171016<\/a><\/span><\/p>\n<p><span style=\"color: #0000ff;\"><a href=\"http:\/\/www.nibb.ac.jp\/imori\/main\/wp-content\/uploads\/2018\/03\/2_\u98fc\u80b2\u5ba4\u3068\u98fc\u80b2\u6c342017116.pdf\"><span style=\"color: #0000ff;\">2_\u98fc\u80b2\u5ba4\u3068\u98fc\u80b2\u6c34 <span style=\"color: #000000;\">ver.2017116<\/span><\/span><\/a><\/span><\/p>\n<p><span style=\"color: #0000ff;\">3_\u98fc\u80b2\u88c5\u7f6e\u3068\u6c34\u69fd\u3001\u305d\u306e\u4ed6\u5668\u5177<\/span><\/p>\n<p><span style=\"color: #0000ff;\"><a href=\"http:\/\/www.nibb.ac.jp\/imori\/main\/wp-content\/uploads\/2018\/03\/4_\u98fc\u6599\u3068\u305d\u306e\u4e0e\u3048\u304b\u305f20171018.pdf\"><span style=\"color: #0000ff;\">4_\u98fc\u6599\u3068\u305d\u306e\u4e0e\u3048\u304b\u305f ver.<\/span>20171018<\/a><\/span><\/p>\n<p><span style=\"color: #0000ff;\"><a href=\"http:\/\/www.nibb.ac.jp\/imori\/main\/wp-content\/uploads\/2018\/03\/5_\u65e5\u5e38\u306e\u4e16\u8a71\u306e\u65b9\u6cd520171020.pdf\"><span style=\"color: #0000ff;\">5_\u65e5\u5e38\u306e\u4e16\u8a71\u306e\u65b9\u6cd5<\/span>20171020<\/a><\/span><\/p>\n<p><span style=\"color: #0000ff;\"><a href=\"http:\/\/www.nibb.ac.jp\/imori\/main\/wp-content\/uploads\/2018\/03\/6_\u75c5\u6c17\u3068\u305d\u306e\u5bfe\u51e6\u6cd520171020.pdf\"><span style=\"color: #0000ff;\">6_\u75c5\u6c17\u3068\u305d\u306e\u5bfe\u51e6\u6cd5 ver.<\/span>20171020<\/a><\/span><\/p>\n<p><a href=\"http:\/\/www.nibb.ac.jp\/imori\/main\/wp-content\/uploads\/2020\/06\/7_\u8a66\u85ac\u3068\u305d\u306e\u8abf\u6574\u6cd5_ver20200624.pdf\"><span style=\"color: #0000ff;\"><span style=\"color: #0000ff;\">7_\u8a66\u85ac\u3068\u305d\u306e\u8abf\u6574\u6cd5 ver.<\/span>20200624<\/span><\/a><\/p>\n<p><span style=\"color: #0000ff;\"><a href=\"http:\/\/www.nibb.ac.jp\/imori\/main\/wp-content\/uploads\/2018\/03\/\uff18_\u5f79\u306b\u7acb\u3064\u60c5\u583120170910.pdf\"><span style=\"color: #0000ff;\">\uff18_\u5f79\u306b\u7acb\u3064\u60c5\u5831 ver.<\/span>20170910<\/a><\/span><\/p>\n<hr \/>\n<h3>Microinjection<\/h3>\n<p>\u9ce5\u53d6\u5927\u5b66\u30a4\u30f3\u30b8\u30a7\u30af\u30b7\u30e7\u30f3\u30d7\u30ed\u30c8\u30b3\u30fc\u30eb\uff08\u8fd1\u65e5up\u4e88\u5b9a\uff09<\/p>\n<pre> \u3053\u306e\u30d7\u30ed\u30c8\u30b3\u30fc\u30eb\u306f\u9ce5\u53d6\u5927\u5b66\u306e\u6797\u3000\u5229\u61b2\u306b\u3088\u3063\u3066\u4f5c\u6210\u3055\u308c\u305f\u98fc\u80b2\u30de\u30cb\u30e5\u30a2\u30eb\u3067\u3059\u3002\u7121\u65ad\u5f15\u7528\u3001\u8ee2\u8f09\u3092\u7981\u3058\u307e\u3059\u3002<\/pre>\n<p><span style=\"color: #0000ff;\"><a href=\"https:\/\/www.ncbi.nlm.nih.gov\/pubmed\/25740495\"><span style=\"color: #0000ff;\">Injection Protocol \u00a0in MIMB<\/span><\/a> <\/span>(PubMed link)<\/p>\n<p><span style=\"color: #0000ff;\"><a href=\"http:\/\/www.nibb.ac.jp\/imori\/main\/?attachment_id=336\">\u30b8\u30a7\u30ce\u30bf\u30a4\u30d4\u30f3\u30b0\u7528\u30e9\u30a4\u30bb\u30fc\u30c8\u4f5c\u88fd\u65b9\u6cd5<\/a><\/span><\/p>\n<hr \/>\n<h3><a href=\"http:\/\/www.nibb.ac.jp\/imori\/main\/?page_id=79\">Protocols for Genome Editing\u00a0\u21d0 <span style=\"color: #0000ff;\">Click Here<\/span><\/a><\/h3>\n","protected":false},"excerpt":{"rendered":"<p>Husbandry (Tottori method) \u3000\u3053\u306e\u30d7\u30ed\u30c8\u30b3\u30fc\u30eb\u306f\u5e83\u5cf6\u5927\u5b66\u4e21\u751f\u985e\u7814\u7a76\u30bb\u30f3\u30bf\u30fc\u5668\u5b98\u518d\u751f\u30e1\u30ab\u30cb\u30ba\u30e0\u7814\u7a76\u30b0\u30eb\u30fc\u30d7\u306e\u30e1\u30f3\u30d0\u30fc\u306b\u3088\u3063\u3066\u4f5c\u6210\u3055\u308c\u305f\u98fc\u80b2\u30de\u30cb\u30e5\u30a2\u30eb\u3067\u3059\u3002\u7121\u65ad\u5f15\u7528\u3001\u8ee2\u8f09\u3092\u7981\u3058\u307e\u3059\u3002 \u3000\u306a\u304a\u3001\u672c\u30de\u30cb &hellip; <\/p>\n<p class=\"link-more\"><a href=\"http:\/\/www.nibb.ac.jp\/imori\/main\/?page_id=84\" class=\"more-link\"><span class=\"screen-reader-text\">&#8220;Protocols (Japanese or English)&#8221; \u306e<\/span>\u7d9a\u304d\u3092\u8aad\u3080<\/a><\/p>\n","protected":false},"author":3,"featured_media":0,"parent":64,"menu_order":3,"comment_status":"closed","ping_status":"closed","template":"","meta":[],"_links":{"self":[{"href":"http:\/\/www.nibb.ac.jp\/imori\/main\/index.php?rest_route=\/wp\/v2\/pages\/84"}],"collection":[{"href":"http:\/\/www.nibb.ac.jp\/imori\/main\/index.php?rest_route=\/wp\/v2\/pages"}],"about":[{"href":"http:\/\/www.nibb.ac.jp\/imori\/main\/index.php?rest_route=\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"http:\/\/www.nibb.ac.jp\/imori\/main\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/www.nibb.ac.jp\/imori\/main\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=84"}],"version-history":[{"count":26,"href":"http:\/\/www.nibb.ac.jp\/imori\/main\/index.php?rest_route=\/wp\/v2\/pages\/84\/revisions"}],"predecessor-version":[{"id":135,"href":"http:\/\/www.nibb.ac.jp\/imori\/main\/index.php?rest_route=\/wp\/v2\/pages\/84\/revisions\/135"}],"up":[{"embeddable":true,"href":"http:\/\/www.nibb.ac.jp\/imori\/main\/index.php?rest_route=\/wp\/v2\/pages\/64"}],"wp:attachment":[{"href":"http:\/\/www.nibb.ac.jp\/imori\/main\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=84"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}