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Yoshiaki Nakamura, Ph. D.

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TEL: 0564-59-5866�@FAX: 0564-59-5868
E-mail: ynsu@nibb.ac.jp

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  1. ���������A�͊ۏ@�O�A�������A�����b�A���������A�c��M��: �������A�����������n�����B�זE�̈ڐA�ɂ�����{�̋@�\�I�Ȕz��q�̐��Y�]���a�������C���t���G���U�������̃��X�N���U��z�肵�ā]. ���{�Ƌ׊w�, 53, J7-J14 (2016).
  2. Rikimaru, K., Nakamura, Y., Takahashi, D., Komatsu, M., Ito, N., Matsubara, K. and Tagami, T.: Production of pure Hinai-dori with normal reproductive capability from transferred primordial germ cells. J. Poult. Sci. 51, 297-306 (2014). ���ӔC����
  3. Miyahara, D., Mori, T., Makino, R., Nakamura, Y., Oishi, I., Ono, T., Nirasawa, K., Tagami, T. and Kagami, H.: Culture conditions for maintain propagation, long-term survival and germline transmission of chicken primordial germ cell-like cells. J. Poult. Sci. 51, 87-95 (2014).
  4. Nakamura, Y., Tasai, M., Takeda, K., Nirasawa, K. and Tagami, T.: Production of functional gametes from cryopreserved primordial germ cells of the Japanese quail. J. Reprod. Dev. 59, 580-587 (2013).
  5. Nakamura, Y., Usui, F., Miyahara, D., Mori, T., Ono, T., Kagami, H., Takeda, K., Nirasawa, K. and Tagami, T.: X-irradiation removes endogenous primordial germ cells (PGCs) and increases germline transmission of donor PGCs in chimeric chickens. J. Reprod. Dev. 58, 432-437 (2012). �@���ӔC����
  6. Takeda, K., Tasai, M., Iwamoto, M., Oe, M., Chikuni, K., Nakamura, Y., Tagami, T., Nirasawa, K., Hanada, H., Pinkert Carl, A. and Onishi, A.: Comparative proteomic analysis of liver mitochondrial proteins derived from cloned adult pigs reconstructed with Meishan pig fibroblast cell and European pig enucleated oocyte. J. Reprod. Dev. 58, 248-253 (2012).
  7. Rikimaru, K., Ito, N., Nakamura, Y., Takahashi, D., Ono, M., Komatsu, M. and Matsubara, K.: Identification of germline chimeric chickens produced by transfer of primordial germ cells using a Hinai-dori-specific microsatellite marker. J. Poult. Sci. 48, 281-291 (2011).
  8. Takeda, K., Tasai, M., Akagi, S., Watanabe, S., Oe, M., Chikuni, K., Ohnishi-Kameyama, M., Hanada, H., Nakamura, Y., Tagami, T. and Nirasawa, K.: Comparison of liver mitochondrial proteins derived from newborn cloned calves and from cloned adult cattle by two-dimensional differential gel electrophoresis. Mol. Reprod. Dev. 78, 263-273 (2011).
  9. Nakamura, Y., Usui, F., Miyahara, D., Mori, T., Watanabe, H., Ono, T., Takeda, K., Nirasawa, K., Kagami, H. and Tagami, T.: Viability and functionality of primordial germ cells after freeze-thaw in chickens. J. Poult. Sci. 48, 57-63 (2011).
  10. Nakamura, Y., Usui, F., Miyahara, D., Mori, T., Ono, T., Takeda, K., Nirasawa, K., Kagami, H. and Tagami, T.: Efficient system for preservation and regeneration of genetic resources in chicken: concurrent storage of primordial germ cells and live animals from early embryos of a rare indigenous fowl (Gifujidori). Reprod. Fertil. Dev. 22, 1237-1246 (2010).

    Reproduction, Fertility and Development�� 22��8���̕\��������܂���
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  11. Nakamura, Y., Usui, F., Ono, T., Takeda, K., Nirasawa, K., Kagami, H. and Tagami, T.: Germline replacement by transfer of primordial germ cells into partially sterilized embryos in the chicken. Biol. Reprod. 83, 130-137 (2010).

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  12. Usui, F., Nakamura, Y., Yamamoto, Y., Tatsumi, K., Tominari, K., Ono, T. and Kagami, H.: A novel concentrating system of chicken stem cells by bone marrow side population cells. J. Poult. Sci. 47, 53-56 (2010).
  13. Usui, F., Nakamura, Y., Yamamoto, Y., Bitoh, A., Ono, T. and Kagami, H.: Analysis of developmental changes in avian DNA methylation using a novel method for quantifying genome-wide DNA methylation. J. Poult. Sci. 46, 286-290 (2009).
  14. Atsumi, Y., Yazawa, S., Usui, F., Nakamura, Y., Yamamoto, Y., Tagami, T., Hiramatsu, K., Kagami, H. and Ono, T.: Depletion of primordial germ cells (PGCs) by X-irradiation to extraembryonic region of chicken embryos and expression of xenotransplanted quail PGCs. J. Poult. Sci. 46, 136-143 (2009).
  15. Nakamura, Y., Usui, F., Atsumi, Y., Otomo, A., Teshima, A., Ono, T., Takeda, K., Nirasawa, K., Kagami, H. and Tagami, T.: Effects of busulfan sustained-release emulsion on depletion and repopulation of primordial germ cells in early chicken embryos. J. Poult. Sci. 46, 127-135 (2009). ���@The Journal of Poultry Science Outstanding Paper Award ��ܘ_��
  16. Usui, F., Yamamoto, Y., Nakamura, Y., Ono, T. and Kagami, H.: Novel system for degeneration of blood vessels by UV irradiation and subsequent regeneration using chick bone marrow cells. Cells Tissues Organs 189, 348-355 (2009).
  17. Nakamura, Y., Yamamoto, Y., Usui, F., Atsumi, Y., Ito, Y., Ono, T., Takeda, K., Nirasawa, K., Kagami, H. and Tagami, T.: Increased proportion of donor primordial germ cells in chimeric gonads by sterilisation of recipient embryos using busulfan sustained-release emulsion in chickens. Reprod. Fertil. Dev. 20, 900-907 (2008).
  18. Nakamura, Y., Yamamoto, Y., Usui, F., Mushika, T., Ono, T., Setioko, A.R., Takeda, K., Nirasawa, K., Kagami, H. and Tagami, T.: Migration and proliferation of primordial germ cells in the early chicken embryo. Poult. Sci. 86, 2182-2193 (2007).

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  19. Yamamoto, Y., Usui, F., Nakamura, Y., Ito, Y., Tagami, T., Nirasawa, K., Matsubara, Y., Ono, T. and Kagami, H.: A novel method to isolate primordial germ cells and its use for the generation of germline chimeras in chicken. Biol. Reprod. 77, 115-119 (2007).
  20. Setioko, A.R., Tagami, T., Tase, H., Nakamura, Y., Takeda, K. and Nirasawa, K.: Cryopreservation of primordial germ cells (PGCs) from White Leghorn embryos using commercial cryoprotectants. J. Poult. Sci. 44, 73-77 (2007).

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  1. Nakamura, Y.: Poultry genetic resource conservation using primordial germ cells. J Reprod Dev. 62, 431-437 (2016). ���ӔC����
  2. Nakamura, Y., Kagami, H. and Tagami, T.: Development, differentiation and manipulation of chicken germ cells. Dev. Growth Diff. 55, 20-40 (2013). ���ӔC����
  3. Tagami, T. and Nakamura, Y.: Improving rates of germline transmission in chimeric chickens using busulfan solubilised sustained-release emulsion. Avian Biol. Res. 4, 74-77 (2011).
  4. ���������A�����T�A�c��M��: ���B�זE�ɂ�铮����`�����̕ۑ��ƌ‘̂ւ̍Đ�. ������`��팤�� 37, 41-58 (2009). ���ӔC����

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  1. Nakamura, Y.: Avian Biotechnology. In: Avian Reproduction: From Behavior to Molecules (Sasanami, T. eds.). Springer SBM Singapore Pte Ltd., Singapore (in press) ���ӔC����
  2. Tagami, T, Miyahara, D, Nakamura, Y.: Avian Primordial Germ Cells. In: Avian Reproduction: From Behavior to Molecules (Sasanami, T. eds.). Springer SBM Singapore Pte Ltd., Singapore (in press)

���ҍu���E�Z�~�i

  1. ��������: ���B�זE�ɂ�铮����`�����̕ۑ���ڎw����: ���ނƂٓ��ނ̐��B�זE�ڐA. ��38��V�w�p�̈�u�z��q�Y������v�Z�~�i (���l�s����w��w��), ���l. (2017. 4)
  2. ��������: ���q���זE�̈ڐA��̐U����������������. ���{�{�Y�w������T�}�[�L�����v�u�A�j�}���T�C�G���X��茤���҂̑ٓ��v, ����. (2016. 9)
  3. Nakamura, Y., Yoshida, S. Post-transplantation dynamics of mouse spermatogenic stem cells: For improvement of its transplantation efficiency. The 12th Annual Conference of the Asian Reproductive Biotechnology Society, Hanoi, Vietnam. (2015. 11)
  4. Nakamura, Y.: Live hands-on primordial germ cells isolation, vitrification and transplantation. Training Workshop in the 12th Annual Conference of the Asian Reproductive Biotechnology Society, Hanoi, Vietnam. (2015. 11)
  5. ��������: ���q���זE�ɂ���`�����̕ۑ� -�P��זE���x���̉�͂���ڐA�̌�������}��-, ���{�{�Y�w���119���� �����V���|�W�E��, �F�s�{. �i2015. 3�j
  6. Nakamura, Y.: Germ cell transplantation in chicken and mouse. Seminar �iRoslin Institute�j, Edinburgh, Scotland �i2015. 3�j
  7. Nakamura, Y.: �n�����B�זE�ɂ��Ƌ׈�`�����̕ۑ�. ��36����{���q�����w��N�� ���[�N�V���b�v�u�j���g�����������y�ѐ��B�H�w�̍őO���v, �_��. �i2013. 12�j
  8. Nakamura, Y.: Poultry New Technology. JICA Group Training Course 2013 �ePoultry production, management and disease preservation technology�f, Shirakawa. �i2013. 11�j
  9. Nakamura, Y.: Poultry New Technology. JICA Group Training Course 2012 �ePoultry production, management and disease preservation technology�f, Shirakawa. �i2012. 12�j
  10. Nakamura, Y.: Germ-cell transplantation in chicken and mice. The 2nd Memorial Lectures for Establishment of International Academic Cooperation Relationship between Fac Agriculture, Shinshu Univ, Japan, and Zhejiang Academy of Agricultural Science �iZAAS�j, China, Minamiminowa, Japan. �i2012. 11�j
  11. Nakamura, Y.: Chicken germplasm preservation using primordial germ cells. Chick7: Avian Model Systems �i7th International Chick meeting�j, S-6-4, Nagoya, Japan. �i2012. 11�j
  12. Nakamura, Y.: Dynamics of spermatogenic stem cells after transplantation in mice. Seminar �iZhejiang Academy of Agricultural Science�j, Hangzhou, China. �i2012. 9�j
  13. ��������: ���B�H�w�𗘗p�����n�����B�זE�ɂ��j���g����`�����ۑ��Ɋւ��錤��. ���{�Ƌ׊w��2012�N�x�t�G���, ���{�Ƌ׊w���܎�܎ҍu��, ���É�. �i2012. 3�j
  14. ��������: ���ގn�����B�זE�̈ڐA�Ɛ��B�H�w�ւ̉��p. ���{�{�Y�w���115����, �V���|�W�E���u���ރo�C�I�T�C�G���X�����̐V���ȓW�J�v, ���É�. �i2012. 3�j
  15. Nakamura, Y.: Genetic conservation at the cellular level in chicken. 2012 International Symposium on Animal Biotechnology. Minamiminowa, Japan. �i2012. 1�j
  16. Nakamura, Y.: Poultry New Technology. JICA Group Training Course 2011 �ePoultry production, management and disease preservation technology�f, Shirakawa. �i2011. 12�j
  17. Nakamura, Y., Kagami, H. and Tagami, T.: Primordial germ cell-mediated genetic resource preservation system in chicken. Korea-Japan Joint Symposium on Avian Biology 2011, Seoul, Republic of Korea. �i2011. 7�j
  18. Nakamura, Y.: Poultry New Technology. JICA Group Training Course 2010 �ePoultry production, management and disease preservation technology�f, Shirakawa. �i2010. 12�j
  19. ��������: �n�����B�זE�ɂ��Ƌ׈�`�����̕ۑ�. ���{�Ƌ׊w��2010�N�x�H�G���, ���J�V���|�W�E�� �e�[�}�i2�j�u�Ƌ׊w�ɂ������茤���҂̑ٓ��v, �얥��. �i2010. 9�j

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